FastQCFastQC Report
Thu 2 Feb 2017
SRR4062108_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062108_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499
Sequences flagged as poor quality0
Sequence length25
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAATATGTTGCCCCCGTTGTCC681.046314817664256No Hit
GTGTAACCCTGTCTGAGCGAGTCCT610.9386059393752885No Hit
TCCCAGCACTGGCCCTTTCCCAATA410.6308662871210955No Hit
GTCTTGATGAGGTGACCACAGCCCT310.47699646099399906No Hit
CTTCTTAATCAAGTGGTGGGCAATG290.44622249576857975No Hit
GTACATGGGAGGTGAAAGTGTGGAA270.4154485305431605No Hit
GCTTTAGGCAGTGGAACTTCTCAGA260.40006154793045084No Hit
GGGAAGAACAGCTCCGCGTCTCCAC260.40006154793045084No Hit
CTGTAGAACAGCGCACAGCATGCCC250.3846745653177412No Hit
ATCCAGTTCACACCAACATTTTTGG250.3846745653177412No Hit
TTTCCCAATAGCTCACTCATGATCG250.3846745653177412No Hit
GTACATGGGGGATGTGAGGGCGATC240.36928758270503154No Hit
TCTCCAGAGTGCCCTGCAGCAATGC230.3539006000923219No Hit
CCTCTGGAGGCTGCACCACAATTCG220.3385136174796122No Hit
GTACAGGTACAAGCTGCTCTATCCA220.3385136174796122No Hit
GAACTAGAGGCAGCCGCCTGGTTCA210.3231266348669026No Hit
ATTAAGAAGTGGGTGGAGACGCGGA210.3231266348669026No Hit
GCCCAGGAGGGATCCAGACGGCTCC200.307739652254193No Hit
ATACAGTACTCTGCGTCCCAGCACT190.2923526696414833No Hit
GCTCTATCCAGTTCACACCAACATT190.2923526696414833No Hit
CTCTGGAGATGACCTGCGAAGGGAG180.27696568702877367No Hit
GGGTTACACTGTGCACCTGGGACAT180.27696568702877367No Hit
GATCCAGACGGCTCCATTTGAGCTA180.27696568702877367No Hit
CTCCTGGGCATTGCTGCAGGGCACT170.261578704416064No Hit
GCCACATGCAGCGCCTCATTTGGAT170.261578704416064No Hit
GGTACAAGCTGCTCTATCCAGTTCA170.261578704416064No Hit
CTCATGATCGCTTGTCACGCAACCG170.261578704416064No Hit
GTCTTACCCCGGGTCTGTGGCTTGC160.24619172180335436No Hit
GCAATAGAGGATACATGCCTGGGGC150.2308047391906447No Hit
GCCTAAAGCCTTCTAATGCTCCACC140.21541775657793505No Hit
GTATCAACGCAGAGTACATGGGGGA140.21541775657793505No Hit
TCTCCACCCACTTCTTAATCAAGTG140.21541775657793505No Hit
GTTCTTCCCTGGCTGCTTAGCTCAA140.21541775657793505No Hit
GCATTAGAAGGCTTTAGGCAGTGGA140.21541775657793505No Hit
CTCTATTGCTGTGCTATGTGACAAT140.21541775657793505No Hit
CCTCAGGGCTGTGGTCACCTCATCA130.20003077396522542No Hit
TATCAACGCAGAGTACATGGGGGAT120.18464379135251577No Hit
ATCTCAGGTTTGGTGCTTTCTCGAA120.18464379135251577No Hit
GTCCTTAAAAGTGAGGAGACACTCC120.18464379135251577No Hit
CTCCAGAGGAGCCCAAGCCACCACG120.18464379135251577No Hit
GGCTTGCGCCGTGGTGGCTTGGGCT120.18464379135251577No Hit
TATCAGGAGTCCATGGAAAGTCTTA120.18464379135251577No Hit
ACCTGAGATACCTCGAATTGTGGTG110.1692568087398061No Hit
ACCCTACGTTCTCCTACAATGGACC110.1692568087398061No Hit
GGGTAAGACTTTCCATGGACTCCTG110.1692568087398061No Hit
GCCTGATACAGTCCTTAAAAGTGAG110.1692568087398061No Hit
CCTCACGGTGGTCCTTGCTGGAAAC110.1692568087398061No Hit
CAATAGGGGGTCCCCTGGCCCTTAT110.1692568087398061No Hit
GACAAGTACAGGTACAAGCTGCTCT110.1692568087398061No Hit
GTCCAGGAAAGAGGCAGAGCCTCGT110.1692568087398061No Hit
TCTCAGAGAGCCTCTGCCCGGGAAG100.1538698261270965No Hit
GCCTGGCACTGGGCACTGTAGAACA100.1538698261270965No Hit
GTTCAGTCTTGATGAGGTGACCACA100.1538698261270965No Hit
CTAATATGTTGCCCCCGTTGTCCTG100.1538698261270965No Hit
AGCCACCACGGCGCAAGCCACAGAC100.1538698261270965No Hit
TATCAACGCAGAGTACATGGGAGGT100.1538698261270965No Hit
GTGTATGGTGCACTTAGAATCGGGT100.1538698261270965No Hit
GCCTGGAAGTGGAAAACATACAGTA100.1538698261270965No Hit
ATCCAGACGGCTCCATTTGAGCTAA100.1538698261270965No Hit
ACTAGAGGCAGCCGCCTGGTTCAGT100.1538698261270965No Hit
GGCCAGCCCCTCTGCCACAGGCCCG100.1538698261270965No Hit
CCCAATAGCTCACTCATGATCGCTT100.1538698261270965No Hit
CCCTGGGCCAGCCCCTCTGCCACAG100.1538698261270965No Hit
GCCCTGAGGAGAAAGGGCTCCTTGG100.1538698261270965No Hit
TTAGAAGGCTTTAGGCAGTGGAACT100.1538698261270965No Hit
GTGGGGAAGCCTCACTCGGCTGCAG100.1538698261270965No Hit
GTACTGTTCCGCGTGGGGCTGACAC90.13848284351438683No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG90.13848284351438683No Hit
CAGGTGCACAGTGTAACCCTGTCTG90.13848284351438683No Hit
GTATCAACGCAGAGTACATGGGAGG90.13848284351438683No Hit
CCCCCTATTGAGGGCCCCTCCAGGT90.13848284351438683No Hit
GTATCAACGCAGAGTACTTTTTTTT90.13848284351438683No Hit
CCTTAAAAGTGAGGAGACACTCCAC90.13848284351438683No Hit
GTACTCTGCGTCCCAGCACTGGCCC90.13848284351438683No Hit
CTTCCCCACTGTTGCTGCCTCCCAA90.13848284351438683No Hit
ACCATATCTCAGCCAGAGGCTTTAT90.13848284351438683No Hit
CATCAAGACTGAACCAGGCGGCTGC90.13848284351438683No Hit
CTCCATGTGCGTCCCTCCCGAAGCT90.13848284351438683No Hit
GAGGAGAAAGGGCTCCTTGGCTCTG90.13848284351438683No Hit
CCCCTATTGAGGGCCCCTCCAGGTC90.13848284351438683No Hit
CTCCTGATAAGGGCCAGGGGACCCC80.12309586090167718No Hit
ACATACAGTACTCTGCGTCCCAGCA80.12309586090167718No Hit
GTGGTGGGCAATGGCTAACTTGGGA80.12309586090167718No Hit
TCCCCACTGTTGCTGCCTCCCAAGT80.12309586090167718No Hit
CCTCTGCCTCCGCCTCCATGGTAGC80.12309586090167718No Hit
GTCGAAGAGGACGACCTTCCCCGAA80.12309586090167718No Hit
GTCTGGATCCCTCCTGGGCATTGCT80.12309586090167718No Hit
CTGTTGCTGCCTCCCAAGTTAGCCA80.12309586090167718No Hit
GCCCTGCAGCAATGCCCAGGAGGGA80.12309586090167718No Hit
GGTGACCACAGCCCTGAGGAGAAAG80.12309586090167718No Hit
GTTCACACCAACATTTTTGGTGGAG80.12309586090167718No Hit
GGCTTTAGGCAGTGGAACTTCTCAG80.12309586090167718No Hit
ATTGAGGGCCCCTCCAGGTCCCAAC80.12309586090167718No Hit
CCACAATTCGAGGTATCTCAGGTTT70.10770887828896752No Hit
GTGCCCTGCAGCAATGCCCAGGAGG70.10770887828896752No Hit
GGCCAGGGGACCCCCTATTGAGGGC70.10770887828896752No Hit
CCGTTGTCCTGGGCAAAAGTGACGC70.10770887828896752No Hit
CTTCTTTATCTTTGTGCCTGATACA70.10770887828896752No Hit
ACCGTGAAACCAGGACACACAGAGA70.10770887828896752No Hit
GCCTCCAGAGGAGCCCAAGCCACCA70.10770887828896752No Hit
CTCCTGGAGACATTGGGGGCCCTAC70.10770887828896752No Hit
CCACTTGATTAAGAAGTGGGTGGAG70.10770887828896752No Hit
AGACTGAACCAGGCGGCTGCCTCTA70.10770887828896752No Hit
GTCCTGGGCAAAAGTGACGCTCTCG70.10770887828896752No Hit
GCCCAAGCCACCACGGCGCAAGCCA70.10770887828896752No Hit
TCCCGAAGCTGCGCGCTCGGTCGAA70.10770887828896752No Hit
ATCAAGTGGTGGGCAATGGCTAACT70.10770887828896752No Hit
CTGTCACCCCATTGATCGCCAGGGT70.10770887828896752No Hit
GGAGATGACCTGCGAAGGGAGGTGA70.10770887828896752No Hit
CATGATCGCTTGTCACGCAACCGTG70.10770887828896752No Hit
GTGGTGGCTTGGGCTCCTCTGGAGG70.10770887828896752No Hit
CCTTGGCTCTGCAGCCGAGTGAGGC70.10770887828896752No Hit
CGTATACCCTTGACCGAAGACCGGT70.10770887828896752No Hit
GTGTCTAATATGTTGCCCCCGTTGT70.10770887828896752No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers

Produced by FastQC (version 0.11.5)