FastQCFastQC Report
Thu 2 Feb 2017
SRR4062108_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062108_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6499
Sequences flagged as poor quality0
Sequence length50
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAACCCTGTCTGAGCGAGTCCTGCAACAGGAAATCAGGATCCAGCTA771.1847976611786428No Hit
GTCTAATATGTTGCCCCCGTTGTCCTGGGCAAAAGTGACGCTCTCGCCTC640.9847668872134174No Hit
TCCCAGCACTGGCCCTTTCCCAATAGCTCACTCATGATCGCTTGTCACGC380.5847053392829666No Hit
GTACAGGTACAAGCTGCTCTATCCAGTTCACACCAACATTTTTGGTGGAG260.40006154793045084No Hit
TCTCCAGAGTGCCCTGCAGCAATGCCCAGGAGGGATCCAGACGGCTCCAT260.40006154793045084No Hit
GTACATGGGAGGTGAAAGTGTGGAAGAAACTGTCCATCGAGAAGTTGCGG220.3385136174796122No Hit
ATCCAGTTCACACCAACATTTTTGGTGGAGCATTAGAAGGCTTTAGGCAG220.3385136174796122No Hit
ATTAAGAAGTGGGTGGAGACGCGGAGCTGTTCTTCCCTGGCTGCTTAGCT220.3385136174796122No Hit
GCTTTAGGCAGTGGAACTTCTCAGAGAGCCTCTGCCCGGGAAGGCTTTGT210.3231266348669026No Hit
GCCCTGAGGAGAAAGGGCTCCTTGGCTCTGCAGCCGAGTGAGGCTTCCCC210.3231266348669026No Hit
GGTACAAGCTGCTCTATCCAGTTCACACCAACATTTTTGGTGGAGCATTA200.307739652254193No Hit
GTCTGAGCGAGTCCTGCAACAGGAAATCAGGATCCAGCTAGCCCAGCTTT200.307739652254193No Hit
CTCTGGAGATGACCTGCGAAGGGAGGTGACAAAGCCTTCCCGGGCAGAGG200.307739652254193No Hit
GCCTGATACAGTCCTTAAAAGTGAGGAGACACTCCACTCCTCACGGTGGT190.2923526696414833No Hit
GTCTTGATGAGGTGACCACAGCCCTGAGGAGAAAGGGCTCCTTGGCTCTG190.2923526696414833No Hit
CCTCTGGAGGCTGCACCACAATTCGAGGTATCTCAGGTTTGGTGCTTTCT190.2923526696414833No Hit
CAATAGGGGGTCCCCTGGCCCTTATCAGGAGTCCATGGAAAGTCTTACCC190.2923526696414833No Hit
GGTGACCACAGCCCTGAGGAGAAAGGGCTCCTTGGCTCTGCAGCCGAGTG190.2923526696414833No Hit
GGGAAGAACAGCTCCGCGTCTCCACCCACTTCTTAATCAAGTGGTGGGCA190.2923526696414833No Hit
GCCACATGCAGCGCCTCATTTGGATGTGTCTGGAGTCTTGGAAGCTTGAC180.27696568702877367No Hit
GTACATGGGGGATGTGAGGGCGATCTGGCTGTGACATCTGTCACCCCATT180.27696568702877367No Hit
GCTCTATCCAGTTCACACCAACATTTTTGGTGGAGCATTAGAAGGCTTTA170.261578704416064No Hit
GCAATAGAGGATACATGCCTGGGGCTGGCATCACTGCTCAGATGATTGAG170.261578704416064No Hit
ATACAGTACTCTGCGTCCCAGCACTGGCCCTTTCCCAATAGCTCACTCAT170.261578704416064No Hit
GCCCAGGAGGGATCCAGACGGCTCCATTTGAGCTAAGCAGCCAGGGAAGA160.24619172180335436No Hit
GAACTAGAGGCAGCCGCCTGGTTCAGTCTTGATGAGGTGACCACAGCCCT160.24619172180335436No Hit
CCCTGGGCCAGCCCCTCTGCCACAGGCCCGCCTTCCTGGGGCCCAAGCCA160.24619172180335436No Hit
GTCTTACCCCGGGTCTGTGGCTTGCGCCGTGGTGGCTTGGGCTCCTCTGG160.24619172180335436No Hit
ACAGTACTCTGCGTCCCAGCACTGGCCCTTTCCCAATAGCTCACTCATGA160.24619172180335436No Hit
CTGTAGAACAGCGCACAGCATGCCCAGGGCTCCTGGAGACATTGGGGGCC150.2308047391906447No Hit
CTTCTTAATCAAGTGGTGGGCAATGGCTAACTTGGGAGGCAGCAACAGTG150.2308047391906447No Hit
GGGTTACACTGTGCACCTGGGACATGAAGACCTCTTCCTGCAGCTGGGTA150.2308047391906447No Hit
GTACTCTGCGTCCCAGCACTGGCCCTTTCCCAATAGCTCACTCATGATCG150.2308047391906447No Hit
CTAATATGTTGCCCCCGTTGTCCTGGGCAAAAGTGACGCTCTCGCCTCTG140.21541775657793505No Hit
GTACTGTTCCGCGTGGGGCTGACACTGATGCGCCTGGCACTGGGCACTGT140.21541775657793505No Hit
GCCTAAAGCCTTCTAATGCTCCACCAAAAATGTTGGTGTGAACTGGATAG140.21541775657793505No Hit
CCTTAAAAGTGAGGAGACACTCCACTCCTCACGGTGGTCCTTGCTGGAAA130.20003077396522542No Hit
GATCCAGACGGCTCCATTTGAGCTAAGCAGCCAGGGAAGAACAGCTCCGC130.20003077396522542No Hit
CTCCTGGGCATTGCTGCAGGGCACTCTGGAGATGACCTGCGAAGGGAGGT130.20003077396522542No Hit
GTCTGGATCCCTCCTGGGCATTGCTGCAGGGCACTCTGGAGATGACCTGC130.20003077396522542No Hit
CTCTATTGCTGTGCTATGTGACAATGCTGTTTCCAGCAAGGACCACCGTG130.20003077396522542No Hit
CCTCAGGGCTGTGGTCACCTCATCAAGACTGAACCAGGCGGCTGCCTCTA130.20003077396522542No Hit
CTGTCACCCCATTGATCGCCAGGGTTGATTCGGCTGATCTGGCTGGCTAG120.18464379135251577No Hit
TCTCCACCCACTTCTTAATCAAGTGGTGGGCAATGGCTAACTTGGGAGGC120.18464379135251577No Hit
CCCCTGGCCCTTATCAGGAGTCCATGGAAAGTCTTACCCCGGGTCTGTGG120.18464379135251577No Hit
GCACCAAACAGCTCCCCACAAGCTCCACTCAGCATCCTTCTTTATCTTTG120.18464379135251577No Hit
GTATCAACGCAGAGTACATGGGAGGTGAAAGTGTGGAAGAAACTGTCCAT120.18464379135251577No Hit
GTCCAGGAAAGAGGCAGAGCCTCGTTGGGACCTGGAGGGGCCCTCAATAG120.18464379135251577No Hit
TATCAACGCAGAGTACATGGGAGGTGAAAGTGTGGAAGAAACTGTCCATC120.18464379135251577No Hit
GTGGTGGCTTGGGCTCCTCTGGAGGCTGCACCACAATTCGAGGTATCTCA110.1692568087398061No Hit
GTTCAGTCTTGATGAGGTGACCACAGCCCTGAGGAGAAAGGGCTCCTTGG110.1692568087398061No Hit
GCCCTGCAGCAATGCCCAGGAGGGATCCAGACGGCTCCATTTGAGCTAAG110.1692568087398061No Hit
CATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCT110.1692568087398061No Hit
GTTCACACCAACATTTTTGGTGGAGCATTAGAAGGCTTTAGGCAGTGGAA110.1692568087398061No Hit
GACCACAGCCCTGAGGAGAAAGGGCTCCTTGGCTCTGCAGCCGAGTGAGG110.1692568087398061No Hit
ACATACAGTACTCTGCGTCCCAGCACTGGCCCTTTCCCAATAGCTCACTC100.1538698261270965No Hit
GTACCATATCTCAGCCAGAGGCTTTATTCCCACAATCCTTCAGTGCTGGC100.1538698261270965No Hit
TTTCCCAATAGCTCACTCATGATCGCTTGTCACGCAACCGTGAAACCAGG100.1538698261270965No Hit
ATTGAGGGCCCCTCCAGGTCCCAACGAGGCTCTGCCTCTTTCCTGGACAC100.1538698261270965No Hit
GAACAGCTCCGCGTCTCCACCCACTTCTTAATCAAGTGGTGGGCAATGGC100.1538698261270965No Hit
CTCCTGATAAGGGCCAGGGGACCCCCTATTGAGGGCCCCTCCAGGTCCCA100.1538698261270965No Hit
TCTGGAGGCTGCACCACAATTCGAGGTATCTCAGGTTTGGTGCTTTCTCG100.1538698261270965No Hit
GTTCTTCCCTGGCTGCTTAGCTCAAATGGAGCCGTCTGGATCCCTCCTGG100.1538698261270965No Hit
CTCCAGAGGAGCCCAAGCCACCACGGCGCAAGCCACAGACCCGGGGTAAG100.1538698261270965No Hit
ACCGTGAAACCAGGACACACAGAGATTCAGGTGAACTTGAAGGAACTAGA90.13848284351438683No Hit
CTCCACTCCTCACGGTGGTCCTTGCTGGAAACAGCATTGTCACATAGCAC90.13848284351438683No Hit
GGTATCAACGCAGAGTACATGGGAGGTGAAAGTGTGGAAGAAACTGTCCA90.13848284351438683No Hit
TCTCAGAGAGCCTCTGCCCGGGAAGGCTTTGTCACCTCCCTTCGCAGGTC90.13848284351438683No Hit
GGCTTTATTTGAGGATTCCCTTGCAAGAAGCCCATGGGGTACCATATCTC90.13848284351438683No Hit
CTGTTGCTGCCTCCCAAGTTAGCCATTGCCCACCACTTGATTAAGAAGTG90.13848284351438683No Hit
GCCTCTGCCCGGGAAGGCTTTGTCACCTCCCTTCGCAGGTCATCTCCAGA90.13848284351438683No Hit
CTCTTCGACCGAGCGCGCAGCTTCGGGAGGGACGCACATGGAGCGGTGAG90.13848284351438683No Hit
ATTTGGATGTGTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTA90.13848284351438683No Hit
CCTGATAAGGGCCAGGGGACCCCCTATTGAGGGCCCCTCCAGGTCCCAAC90.13848284351438683No Hit
GCTCAAATGGAGCCGTCTGGATCCCTCCTGGGCATTGCTGCAGGGCACTC90.13848284351438683No Hit
ACGCAGAGTACATGGGAGGTGAAAGTGTGGAAGAAACTGTCCATCGAGAA90.13848284351438683No Hit
GCCTCCGCCTCCATGGTAGCTGGGACAAGTACAGGTACAAGCTGCTCTAT90.13848284351438683No Hit
CACAGAGATTCAGGTGAACTTGAAGGAACTAGAGGCAGCCGCCTGGTTCA90.13848284351438683No Hit
CTCCTACAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAGCAGGGGAGCG90.13848284351438683No Hit
AACCAGGACACACAGAGATTCAGGTGAACTTGAAGGAACTAGAGGCAGCC90.13848284351438683No Hit
CCGTTGTCCTGGGCAAAAGTGACGCTCTCGCCTCTGCCTCCGCCTCCATG90.13848284351438683No Hit
CCCAATAGCTCACTCATGATCGCTTGTCACGCAACCGTGAAACCAGGACA90.13848284351438683No Hit
TTAGAAGGCTTTAGGCAGTGGAACTTCTCAGAGAGCCTCTGCCCGGGAAG90.13848284351438683No Hit
GGAAAGAGGCAGAGCCTCGTTGGGACCTGGAGGGGCCCTCAATAGGGGGT90.13848284351438683No Hit
CCAGAGTGCCCTGCAGCAATGCCCAGGAGGGATCCAGACGGCTCCATTTG80.12309586090167718No Hit
GGGTAAGACTTTCCATGGACTCCTGATAAGGGCCAGGGGACCCCCTATTG80.12309586090167718No Hit
GTCTGGAGTCTTGGAAGCTTGACTACCCTACGTTCTCCTACAATGGACCT80.12309586090167718No Hit
GCCTGGCACTGGGCACTGTAGAACAGCGCACAGCATGCCCAGGGCTCCTG80.12309586090167718No Hit
CTTCTCAGTCAGGCTTTATTTGAGGATTCCCTTGCAAGAAGCCCATGGGG80.12309586090167718No Hit
CCCCTATTGAGGGCCCCTCCAGGTCCCAACGAGGCTCTGCCTCTTTCCTG80.12309586090167718No Hit
CTCATGATCGCTTGTCACGCAACCGTGAAACCAGGACACACAGAGATTCA80.12309586090167718No Hit
GGCCAGCCCCTCTGCCACAGGCCCGCCTTCCTGGGGCCCAAGCCATCTTC80.12309586090167718No Hit
ACTCTGCGTCCCAGCACTGGCCCTTTCCCAATAGCTCACTCATGATCGCT80.12309586090167718No Hit
ACCCTACGTTCTCCTACAATGGACCTTGAGAGCTTGTTTGGAGGTTCTAG80.12309586090167718No Hit
GCACTGGCCCTTTCCCAATAGCTCACTCATGATCGCTTGTCACGCAACCG80.12309586090167718No Hit
GTCCTTAAAAGTGAGGAGACACTCCACTCCTCACGGTGGTCCTTGCTGGA80.12309586090167718No Hit
GTGTGGAAGAAACTGTCCATCGAGAAGTTGCGGAAGAGGTGGGCCTGGAA80.12309586090167718No Hit
GACCTGGAGGGGCCCTCAATAGGGGGTCCCCTGGCCCTTATCAGGAGTCC80.12309586090167718No Hit
ACCATATCTCAGCCAGAGGCTTTATTCCCACAATCCTTCAGTGCTGGCTT80.12309586090167718No Hit
GTCACATAGCACAGCAATAGAGGATACATGCCTGGGGCTGGCATCACTGC80.12309586090167718No Hit
GTGTATGGTGCACTTAGAATTGGGTGTCCAGGAAAGAGGCAGAGCCTCGT80.12309586090167718No Hit
CCTGGGGCCCAAGCCATCTTCGAGTCCCAGCAGCTGGCAGGGGTTCGAGA70.10770887828896752No Hit
CATCAAGACTGAACCAGGCGGCTGCCTCTAGTTCCTTCAAGTTCACCTGA70.10770887828896752No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70.10770887828896752No Hit
CAGCATGCCCAGGGCTCCTGGAGACATTGGGGGCCCTACGAGCCATTCCC70.10770887828896752No Hit
CTCCTTGGCTCTGCAGCCGAGTGAGGCTTCCCCACTGTTGCTGCCTCCCA70.10770887828896752No Hit
GTGCTATGTGACAATGCTGTTTCCAGCAAGGACCACCGTGAGGAGTGGAG70.10770887828896752No Hit
GAACTGGATAGAGCAGCTTGTACCTGTACTTGTCCCAGCTACCATGGAGG70.10770887828896752No Hit
TTCCTGGGGCCCAAGCCATCTTCGAGTCCCAGCAGCTGGCAGGGGTTCGA70.10770887828896752No Hit
CCACTTGATTAAGAAGTGGGTGGAGACGCGGAGCTGTTCTTCCCTGGCTG70.10770887828896752No Hit
GTATCAACGCAGAGTACATGGGGGGCTAAGGTACTGTTCCGCGTGGGGCT70.10770887828896752No Hit
AAGTTAGCCATTGCCCACCACTTGATTAAGAAGTGGGTGGAGACGCGGAG70.10770887828896752No Hit
CTGCAGCAATGCCCAGGAGGGATCCAGACGGCTCCATTTGAGCTAAGCAG70.10770887828896752No Hit
GTCCTGCAACAGGAAATCAGGATCCAGCTAGCCCAGCTTTCGAAGTCACT70.10770887828896752No Hit
TCGCAGGTCATCTCCAGAGTGCCCTGCAGCAATGCCCAGGAGGGATCCAG70.10770887828896752No Hit
ATCAAGTGGTGGGCAATGGCTAACTTGGGAGGCAGCAACAGTGGGGAAGC70.10770887828896752No Hit
GTGCTGGCTTTGATGCTGGCAATGGCAAGCACCAAACAGCTCCCCACAAG70.10770887828896752No Hit
TCCCCACTGTTGCTGCCTCCCAAGTTAGCCATTGCCCACCACTTGATTAA70.10770887828896752No Hit
CATGATCGCTTGTCACGCAACCGTGAAACCAGGACACACAGAGATTCAGG70.10770887828896752No Hit
CCACAGCCCTGAGGAGAAAGGGCTCCTTGGCTCTGCAGCCGAGTGAGGCT70.10770887828896752No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers