FastQCFastQC Report
Thu 2 Feb 2017
SRR4062107_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062107_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595007
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13780.23159391402117957No Hit
TATCAACGCAGAGTACTTTTTTTTT9840.1653762056580847No Hit
GCGCAAGACGGACCAGAGCGAAAGC8020.1347883302213251No Hit
GAATAGGACCGCGGTTCTATTTTGT7580.12739345923661402No Hit
GATTAAGAGGGACGGCCGGGGGCAT7390.12420021949321605No Hit
GGGTAGGCACACGCTGAGCCAGTCA7090.1191582620036403No Hit
ATCAGATACCGTCGTAGTTCCGACC6980.11730954425746253No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG6970.11714147900781No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA6500.10924241227414132No Hit
TCGTAGTTCCGACCATAAACGATGC6480.10890628177483626No Hit
GGTATCAACGCAGAGTACTTTTTTT6480.10890628177483626No Hit
GAACTACGACGGTATCTGATCGTCT6410.10772982502726858No Hit
GTCTTGCGCCGGTCCAAGAATTTCA6370.10705756402865849No Hit
GTATCTGATCGTCTTCGAACCTCCG6210.10436852003421809No Hit
GAATAACGCCGCCGCATCGCCAGTC6200.10420045478456556No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC6070.10201560653908272No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCGA453.5301367E-516.89046110
TAATACT601.464241E-615.8321424
GTATTAG456.757494E-414.7766661
TAGAGTG1058.731149E-1114.4763195
GGTTCCG400.00527077314.2513268
TATTATA400.005276662314.2489282
TAGGGTC500.001498497213.2994
CTAGGGT500.001498497213.2993
CGGTTCT1450.013.10356312
GGTTCTA1450.013.10246213
AATACTG604.0851143E-412.66677955
CGTTATT604.088016E-412.6657132
CGCCACT905.3910844E-712.66571317
CAGTTAA751.4760901E-512.66571216
CCTCTAC751.4760901E-512.6657121
AAGACGG1155.295078E-912.3914145
CGGTCCA1700.012.29526110
TAGACAG853.93136E-612.2942275
GGGTCTG701.08777014E-412.2154237
GCGGTCG550.00306247812.0920339
Produced by FastQC (version 0.11.5)