##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062107_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	595007
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.283589940958677	32.0	32.0	32.0	32.0	32.0
2	30.85350928644537	32.0	32.0	32.0	32.0	32.0
3	30.894219731868702	32.0	32.0	32.0	32.0	32.0
4	30.892157571255463	32.0	32.0	32.0	32.0	32.0
5	30.826501200826208	32.0	32.0	32.0	32.0	32.0
6	34.481456520679586	36.0	36.0	36.0	32.0	36.0
7	34.410880880393	36.0	36.0	36.0	32.0	36.0
8	34.354595828284374	36.0	36.0	36.0	32.0	36.0
9	34.48950852679044	36.0	36.0	36.0	32.0	36.0
10	34.21062609347453	36.0	36.0	36.0	32.0	36.0
11	34.47979267470803	36.0	36.0	36.0	32.0	36.0
12	34.30218804148522	36.0	36.0	36.0	32.0	36.0
13	34.39297857000002	36.0	36.0	36.0	32.0	36.0
14	34.30709554677508	36.0	36.0	36.0	32.0	36.0
15	34.26438848618588	36.0	36.0	36.0	32.0	36.0
16	34.25004579778053	36.0	36.0	36.0	32.0	36.0
17	34.19369856152953	36.0	36.0	36.0	32.0	36.0
18	34.213972272595115	36.0	36.0	36.0	32.0	36.0
19	34.18826837331326	36.0	36.0	36.0	32.0	36.0
20	34.189592727480516	36.0	36.0	36.0	32.0	36.0
21	34.15479481753996	36.0	36.0	36.0	32.0	36.0
22	34.130942997309276	36.0	36.0	36.0	32.0	36.0
23	34.10056016147709	36.0	36.0	36.0	32.0	36.0
24	34.07949318243315	36.0	36.0	36.0	32.0	36.0
25	33.73599470258333	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	7.0
5	51.0
6	135.0
7	21.0
8	95.0
9	85.0
10	37.0
11	3.0
12	22.0
13	28.0
14	127.0
15	143.0
16	231.0
17	286.0
18	373.0
19	512.0
20	800.0
21	1082.0
22	1626.0
23	2296.0
24	3285.0
25	4632.0
26	6362.0
27	8145.0
28	11033.0
29	14713.0
30	19516.0
31	26541.0
32	38348.0
33	55031.0
34	122727.0
35	276713.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.179864337046475	17.699208744815163	11.661770341039725	25.45915657709864
2	16.689047355745394	19.381452655178848	37.59384343377473	26.33565655530103
3	19.249586137931615	22.820816631793008	28.183796774817015	29.745800455458358
4	12.85280631454389	15.452210628227196	34.778735477624785	36.91624757960413
5	15.035877364644486	36.44780465497878	32.84090221316602	15.675415767210712
6	34.980003834420316	34.86883928060893	16.244992549788602	13.906164335182153
7	30.95314605664063	30.088041468490644	19.669268261496452	19.289544213372274
8	28.379219608054267	32.93178636804135	18.84085280499623	19.848141218908154
9	26.877322043937106	14.330039844927848	18.74899041567952	40.043647695455526
10	17.02493247279979	26.259455911680313	29.941686791595494	26.773924823924407
11	37.414313387896755	21.25314093080948	21.61280170186697	19.7197439794268
12	24.71837503996769	23.62452249129125	28.029045992292545	23.628056476448513
13	29.380653086637892	19.282462915751655	25.197936912604856	26.138947085005594
14	23.771785475116015	19.666407546128504	24.61611460531231	31.945692373443173
15	25.407678663386164	27.247407517106513	21.84383509082525	25.501078728682064
16	25.806630736017418	25.791486690908354	23.555047762635603	24.846834810438622
17	23.8454224966976	25.9813382918395	25.01644889064643	25.15679032081647
18	24.77054649893076	24.99028345394189	26.059860653793155	24.179309393334197
19	25.781999131953203	24.747913154946353	24.897970856506483	24.572116856593958
20	25.888493946617185	24.219923594651288	24.655107736251832	25.23647472247969
21	26.904372195989307	24.12084224196373	24.284351706840315	24.69043385520665
22	26.03941823198378	24.087854036457237	25.00391154030569	24.86881619125329
23	24.48849836171458	24.33022047890413	25.29183010273765	25.889451056643637
24	24.706371181816042	24.838424245278894	25.36529073542622	25.089913837478843
25	24.923125576137643	24.389714639447178	25.27099496026753	25.416164824147653
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	737.0
1	737.0
2	503.5
3	270.0
4	270.0
5	270.0
6	523.0
7	776.0
8	776.0
9	776.0
10	886.0
11	996.0
12	996.0
13	996.0
14	1211.5
15	1427.0
16	1427.0
17	1427.0
18	2512.5
19	3598.0
20	3598.0
21	3598.0
22	5674.5
23	7751.0
24	7751.0
25	7751.0
26	11996.0
27	16241.0
28	16241.0
29	16241.0
30	20303.0
31	24365.0
32	24365.0
33	24365.0
34	29889.5
35	35414.0
36	35414.0
37	35414.0
38	40916.0
39	46418.0
40	46418.0
41	46418.0
42	53167.5
43	59917.0
44	59917.0
45	59917.0
46	67754.5
47	75592.0
48	75592.0
49	75592.0
50	78317.0
51	81042.0
52	81042.0
53	81042.0
54	75584.5
55	70127.0
56	70127.0
57	70127.0
58	65357.0
59	60587.0
60	60587.0
61	60587.0
62	53521.0
63	46455.0
64	46455.0
65	46455.0
66	38340.0
67	30225.0
68	30225.0
69	30225.0
70	23062.5
71	15900.0
72	15900.0
73	15900.0
74	12170.5
75	8441.0
76	8441.0
77	8441.0
78	6806.0
79	5171.0
80	5171.0
81	5171.0
82	3591.5
83	2012.0
84	2012.0
85	2012.0
86	1468.5
87	925.0
88	925.0
89	925.0
90	615.0
91	305.0
92	305.0
93	305.0
94	190.0
95	75.0
96	75.0
97	75.0
98	157.5
99	240.0
100	240.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	5.041957489575754E-4
2	0.0
3	3.361304993050502E-4
4	0.010588110728109081
5	0.034117245679462595
6	0.06604964311344236
7	0.10554497678178576
8	0.10739369452796353
9	0.11814987050572515
10	0.1347883302213251
11	0.14050254870951098
12	0.13058669898001202
13	0.12638506773869887
14	0.11579695701058978
15	0.13260348197584232
16	0.11999858825190292
17	0.12470441524217361
18	0.11092306477066656
19	0.09562912705228678
20	0.0917636263102787
21	0.0917636263102787
22	0.10285593278734535
23	0.08117551558216962
24	0.09226782205923628
25	0.08924264756549083
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	595007.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	52.40723929014867
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.1257327677942	39.37132254009554
2	14.508625355509446	15.207140015546038
3	4.545003408385786	7.145732435934455
4	1.9755235609270265	4.14126943923317
5	1.0309841298162445	2.701551599781281
6	0.6159040758334078	1.9366699369187563
7	0.41195736767734487	1.5112683841644456
8	0.29022750867641034	1.216801799663067
9	0.2033761924984001	0.9592546307564694
>10	1.0950723804500881	10.423080049167606
>50	0.09879663009769349	3.6053031471110173
>100	0.09203854719057375	9.339685906654552
>500	0.006436262041298768	2.2085157814951857
>1k	3.218131020649385E-4	0.23240433347845482
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1378	0.23159391402117957	No Hit
TATCAACGCAGAGTACTTTTTTTTT	984	0.1653762056580847	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	802	0.1347883302213251	No Hit
GAATAGGACCGCGGTTCTATTTTGT	758	0.12739345923661402	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	739	0.12420021949321605	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	709	0.1191582620036403	No Hit
ATCAGATACCGTCGTAGTTCCGACC	698	0.11730954425746253	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	697	0.11714147900781	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	650	0.10924241227414132	No Hit
TCGTAGTTCCGACCATAAACGATGC	648	0.10890628177483626	No Hit
GGTATCAACGCAGAGTACTTTTTTT	648	0.10890628177483626	No Hit
GAACTACGACGGTATCTGATCGTCT	641	0.10772982502726858	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	637	0.10705756402865849	No Hit
GTATCTGATCGTCTTCGAACCTCCG	621	0.10436852003421809	No Hit
GAATAACGCCGCCGCATCGCCAGTC	620	0.10420045478456556	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	607	0.10201560653908272	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.680652496525251E-4	0.0
10	0.0	0.0	0.0	1.680652496525251E-4	0.0
11	0.0	0.0	0.0	1.680652496525251E-4	0.0
12	0.0	0.0	0.0	1.680652496525251E-4	0.0
13	0.0	0.0	0.0	1.680652496525251E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATCGA	45	3.5301367E-5	16.890461	10
TAATACT	60	1.464241E-6	15.832142	4
GTATTAG	45	6.757494E-4	14.776666	1
TAGAGTG	105	8.731149E-11	14.476319	5
GGTTCCG	40	0.005270773	14.251326	8
TATTATA	40	0.0052766623	14.248928	2
TAGGGTC	50	0.0014984972	13.299	4
CTAGGGT	50	0.0014984972	13.299	3
CGGTTCT	145	0.0	13.103563	12
GGTTCTA	145	0.0	13.102462	13
AATACTG	60	4.0851143E-4	12.6667795	5
CGTTATT	60	4.088016E-4	12.665713	2
CGCCACT	90	5.3910844E-7	12.665713	17
CAGTTAA	75	1.4760901E-5	12.665712	16
CCTCTAC	75	1.4760901E-5	12.665712	1
AAGACGG	115	5.295078E-9	12.391414	5
CGGTCCA	170	0.0	12.295261	10
TAGACAG	85	3.93136E-6	12.294227	5
GGGTCTG	70	1.08777014E-4	12.215423	7
GCGGTCG	55	0.003062478	12.092033	9
>>END_MODULE