FastQCFastQC Report
Thu 2 Feb 2017
SRR4062107_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062107_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences595007
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT8800.1478974196942221No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA7370.12386408899391099No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA7370.12386408899391099No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT7220.12134311024912313No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG7170.1205027840008605No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT7170.1205027840008605No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6830.11478856551267465No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC6680.11226758676788676No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC6200.10420045478456556No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA6140.10319206328665041No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC5990.10067108454186253No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATAAT300.005734501429.341911
GTGATAC300.005743931529.3320453
TACGAGT300.005743931529.33204516
ATTTCGT609.726389E-625.67201242
TGTAGGA1400.025.1438662
GTATCAA10850.024.7445581
CGTACCG450.001397053524.4454252
TAGGTTG450.001397737724.443375
GGTATCA5000.022.446561
GATTTCG703.206657E-522.0045841
CGCGGGA2050.020.3944944
GGAATAT2050.020.38934912
AACGAAT654.929576E-420.306831
GCGAGAA1201.4284524E-820.16578120
TTGATTA2200.019.99912336
GTAAACG2100.019.90388927
GATTAAT2000.019.80245838
GTAGGAC1800.019.5546973
TTACACC901.0009382E-519.5546974
AACGCTT2150.019.44100630
Produced by FastQC (version 0.11.5)