FastQCFastQC Report
Thu 2 Feb 2017
SRR4062106_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062106_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132481
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT19030.16803813927121072No Hit
GCGCAAGACGGACCAGAGCGAAAGC13090.11558692816921433No Hit
GATTAAGAGGGACGGCCGGGGGCAT11620.10260657794700309No Hit
TATCAACGCAGAGTACTTTTTTTTT11470.10128205241412438No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGTAA957.365634E-813.00190510
GAGCGGT1102.737579E-912.9558737
GTATCAA11750.012.9323491
CGACCAT2100.012.66852410
CGGTCCA1501.8189894E-1212.66852410
AACCGCG751.4761863E-512.6679637
GTAGCAC751.4775291E-512.6668433
GGTATCA4950.012.6629251
GACGTGA701.089352E-412.2155377
CGAACGA701.0911519E-412.21337716
AACCTAT550.003065205912.0921486
GTTGAAC550.003067236612.0910793
TCGGCGT1103.8109647E-812.09054313
ATCGCCA1501.4551915E-1112.03243716
CGCATCG1603.6379788E-1211.87464113
GTTATAG1603.6379788E-1211.8714911
CCGACCA2250.011.8234339
TAGAGTG1459.640644E-1111.7943115
TCGCGTA1052.713532E-711.7631099
GCGTAAC1052.713532E-711.76310911
Produced by FastQC (version 0.11.5)