##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062106_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1132481
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.223226703141158	32.0	32.0	32.0	32.0	32.0
2	30.752269574500588	32.0	32.0	32.0	32.0	32.0
3	30.770077378781632	32.0	32.0	32.0	32.0	32.0
4	30.78504451730316	32.0	32.0	32.0	32.0	32.0
5	30.708936397166926	32.0	32.0	32.0	32.0	32.0
6	34.34330377286683	36.0	36.0	36.0	32.0	36.0
7	34.280662545331886	36.0	36.0	36.0	32.0	36.0
8	34.24217624843154	36.0	36.0	36.0	32.0	36.0
9	34.37515331383043	36.0	36.0	36.0	32.0	36.0
10	34.05679477183282	36.0	36.0	36.0	32.0	36.0
11	34.3661606684792	36.0	36.0	36.0	32.0	36.0
12	34.1738227837818	36.0	36.0	36.0	32.0	36.0
13	34.26314260459999	36.0	36.0	36.0	32.0	36.0
14	34.155554044615315	36.0	36.0	36.0	32.0	36.0
15	34.118785215822605	36.0	36.0	36.0	32.0	36.0
16	34.11453790394717	36.0	36.0	36.0	32.0	36.0
17	34.056678213585926	36.0	36.0	36.0	32.0	36.0
18	34.073477612427936	36.0	36.0	36.0	32.0	36.0
19	34.06348715784194	36.0	36.0	36.0	32.0	36.0
20	34.05256247124676	36.0	36.0	36.0	32.0	36.0
21	34.036657568647954	36.0	36.0	36.0	32.0	36.0
22	34.00051921400889	36.0	36.0	36.0	32.0	36.0
23	33.976145295152854	36.0	36.0	36.0	32.0	36.0
24	33.94800442568131	36.0	36.0	36.0	32.0	36.0
25	33.58312148283282	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	14.0
5	106.0
6	226.0
7	48.0
8	192.0
9	156.0
10	71.0
11	25.0
12	56.0
13	45.0
14	151.0
15	218.0
16	333.0
17	447.0
18	636.0
19	988.0
20	1429.0
21	2066.0
22	3283.0
23	4877.0
24	6956.0
25	9756.0
26	13518.0
27	17768.0
28	23506.0
29	31717.0
30	40549.0
31	55390.0
32	78059.0
33	110125.0
34	236630.0
35	493140.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.01545734945972	17.306844943490827	11.704670583176082	25.973027123873372
2	16.738323624544034	19.508088008695967	37.1269577625722	26.626630604187806
3	19.03469322339066	22.890781116304286	28.24351041838673	29.831015241918323
4	12.792586096812053	15.61006712535512	35.440085697203806	36.15726108062902
5	15.159422235727257	36.1452466592703	33.2499770335875	15.445354071414943
6	34.69180745034283	35.08261468862656	16.55289107231215	13.672686788718455
7	30.846499392743688	30.2251886737416	20.165152503540117	18.763159429974596
8	28.191114151909076	32.86298199741532	19.216340399652783	19.729563451022823
9	27.24317605581507	14.328956720687716	18.62267540338978	39.805191820107424
10	16.30562120420399	26.60569520813495	31.031077230219424	26.05760635744164
11	37.13246379348915	21.263140582681885	22.123130873969778	19.48126474985919
12	24.45115233694605	23.67900789208899	28.516736427569793	23.353103343395173
13	29.23190092055891	19.611034682755523	25.01326198972284	26.143802406962724
14	23.565521417615884	19.633396540826293	25.006121844597967	31.794960196959853
15	25.157467365971485	27.248839109199775	21.926247626053467	25.66744589877527
16	25.81409431868914	25.837875111058068	23.49772137574978	24.850309194503012
17	23.959590387201324	26.100659790278108	25.089977429390593	24.849772393129975
18	24.81142524766873	25.26234277334168	25.88669691641716	24.03953506257243
19	25.60005303164221	25.16174650875022	25.2221141948029	24.016086264804667
20	25.718348772238077	24.51935526921673	24.752684389663166	25.00961156888203
21	26.513884715323567	24.41571067470348	24.490304562247008	24.580100047725946
22	25.96224473831451	24.38163980911115	24.913045515093177	24.743069937481163
23	24.479946834787512	24.534472991877635	25.360672412680824	25.624907760654033
24	24.633587374706458	25.109439892243802	25.252089588251465	25.004883144798278
25	24.87096682963733	24.632785737769247	25.189307569121127	25.3069398634723
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	491.0
1	491.0
2	421.0
3	351.0
4	351.0
5	351.0
6	802.5
7	1254.0
8	1254.0
9	1254.0
10	1471.5
11	1689.0
12	1689.0
13	1689.0
14	2404.5
15	3120.0
16	3120.0
17	3120.0
18	5361.5
19	7603.0
20	7603.0
21	7603.0
22	11856.0
23	16109.0
24	16109.0
25	16109.0
26	23594.5
27	31080.0
28	31080.0
29	31080.0
30	39036.5
31	46993.0
32	46993.0
33	46993.0
34	58384.0
35	69775.0
36	69775.0
37	69775.0
38	80910.5
39	92046.0
40	92046.0
41	92046.0
42	105148.5
43	118251.0
44	118251.0
45	118251.0
46	131387.0
47	144523.0
48	144523.0
49	144523.0
50	149229.0
51	153935.0
52	153935.0
53	153935.0
54	143284.5
55	132634.0
56	132634.0
57	132634.0
58	122795.5
59	112957.0
60	112957.0
61	112957.0
62	99726.0
63	86495.0
64	86495.0
65	86495.0
66	70733.5
67	54972.0
68	54972.0
69	54972.0
70	41697.5
71	28423.0
72	28423.0
73	28423.0
74	21453.0
75	14483.0
76	14483.0
77	14483.0
78	11691.5
79	8900.0
80	8900.0
81	8900.0
82	6217.0
83	3534.0
84	3534.0
85	3534.0
86	2618.5
87	1703.0
88	1703.0
89	1703.0
90	1140.0
91	577.0
92	577.0
93	577.0
94	356.0
95	135.0
96	135.0
97	135.0
98	291.5
99	448.0
100	448.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.830170219191315E-4
2	1.7660340438382631E-4
3	0.0010596204263029578
4	0.010772807667413405
5	0.03470256896142187
6	0.0636655272803694
7	0.102694879649195
8	0.10587374092810387
9	0.11629334178674962
10	0.1289204852001932
11	0.13580801797116243
12	0.1302450107330719
13	0.1261831324322439
14	0.11355598901880032
15	0.1283023732848498
16	0.11620504008455772
17	0.12044352178976954
18	0.108787697100437
19	0.09545414006945811
20	0.09112735666205438
21	0.09015603793794333
22	0.10084054390316483
23	0.08070775580340862
24	0.09165716687520585
25	0.08733038346780211
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1132481.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.59689075731607
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.93802843538484	44.67646971955384
2	12.889154389299826	14.589721258507666
3	3.720155334435302	6.316476751898444
4	1.4734288286280584	3.3356596181016953
5	0.7763518781894587	2.196955121956297
6	0.4515443000639242	1.5333602053684
7	0.3071508463441379	1.2168648027589444
8	0.2198356965220265	0.9953613520492498
9	0.17158855679758644	0.8740240923850671
>10	0.889249525042912	9.196534569257489
>50	0.08216672770451292	3.2725633653785975
>100	0.07303852753206067	8.232111622818799
>500	0.006739604233664048	2.511476169485861
>1k	0.0015673498217823364	1.0524213504796642
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1903	0.16803813927121072	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1309	0.11558692816921433	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1162	0.10260657794700309	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1147	0.10128205241412438	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	8.830170219191316E-5
2	0.0	0.0	0.0	0.0	8.830170219191316E-5
3	0.0	0.0	0.0	0.0	8.830170219191316E-5
4	0.0	0.0	0.0	0.0	8.830170219191316E-5
5	0.0	8.830170219191316E-5	0.0	0.0	8.830170219191316E-5
6	0.0	8.830170219191316E-5	0.0	0.0	8.830170219191316E-5
7	0.0	8.830170219191316E-5	0.0	0.0	8.830170219191316E-5
8	0.0	8.830170219191316E-5	0.0	0.0	8.830170219191316E-5
9	0.0	8.830170219191316E-5	0.0	8.830170219191316E-5	8.830170219191316E-5
10	0.0	8.830170219191316E-5	0.0	1.7660340438382631E-4	8.830170219191316E-5
11	0.0	8.830170219191316E-5	0.0	2.6490510657573946E-4	8.830170219191316E-5
12	0.0	8.830170219191316E-5	0.0	2.6490510657573946E-4	1.7660340438382631E-4
13	0.0	8.830170219191316E-5	0.0	2.6490510657573946E-4	1.7660340438382631E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGTAA	95	7.365634E-8	13.001905	10
GAGCGGT	110	2.737579E-9	12.955873	7
GTATCAA	1175	0.0	12.932349	1
CGACCAT	210	0.0	12.668524	10
CGGTCCA	150	1.8189894E-12	12.668524	10
AACCGCG	75	1.4761863E-5	12.667963	7
GTAGCAC	75	1.4775291E-5	12.666843	3
GGTATCA	495	0.0	12.662925	1
GACGTGA	70	1.089352E-4	12.215537	7
CGAACGA	70	1.0911519E-4	12.213377	16
AACCTAT	55	0.0030652059	12.092148	6
GTTGAAC	55	0.0030672366	12.091079	3
TCGGCGT	110	3.8109647E-8	12.090543	13
ATCGCCA	150	1.4551915E-11	12.032437	16
CGCATCG	160	3.6379788E-12	11.874641	13
GTTATAG	160	3.6379788E-12	11.871491	1
CCGACCA	225	0.0	11.823433	9
TAGAGTG	145	9.640644E-11	11.794311	5
TCGCGTA	105	2.713532E-7	11.763109	9
GCGTAAC	105	2.713532E-7	11.763109	11
>>END_MODULE