FastQCFastQC Report
Thu 2 Feb 2017
SRR4062106_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062106_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1132481
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT12930.11417410093414371No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT12320.108787697100437No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA12310.10869939539824508No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA13950.026.9803031
CGAATGC3150.020.9519643
CTAGCGG3400.020.7063829
AATACGA3300.020.6662539
TAGGACG1401.8553692E-1020.4281624
GGTATCA6550.020.1620581
TAGCGGC3500.020.11476930
CGCAATA3500.020.11388236
CAATACG3450.019.7677238
GCGCAAT3600.019.55516435
TCTAGCG3500.019.48618328
CGGATCG808.986273E-519.25046326
TCTAGAT2900.018.9668122
AACGCAG20200.018.7322986
TACGAAT3650.018.68455541
ATACGAA3650.018.68455540
TTAGGAC2250.018.5774043
TCAACGC20350.018.4861154
CAACGCA20900.018.2101615
TATACTG1355.525908E-817.9255685
Produced by FastQC (version 0.11.5)