##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062106_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1132481
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.286638804536235	32.0	32.0	32.0	32.0	32.0
2	31.440317320996996	32.0	32.0	32.0	32.0	32.0
3	31.56842896260511	32.0	32.0	32.0	32.0	32.0
4	31.643268187280846	32.0	32.0	32.0	32.0	32.0
5	31.620764498477236	32.0	32.0	32.0	32.0	32.0
6	35.25488109734292	36.0	36.0	36.0	36.0	36.0
7	35.25055961203764	36.0	36.0	36.0	36.0	36.0
8	35.21016953043804	36.0	36.0	36.0	36.0	36.0
9	35.293309998136834	36.0	36.0	36.0	36.0	36.0
10	35.16748537061549	36.0	36.0	36.0	36.0	36.0
11	35.29287290471098	36.0	36.0	36.0	36.0	36.0
12	35.20172965374253	36.0	36.0	36.0	36.0	36.0
13	35.23823975854783	36.0	36.0	36.0	36.0	36.0
14	35.209940829029364	36.0	36.0	36.0	36.0	36.0
15	35.1844216370959	36.0	36.0	36.0	36.0	36.0
16	35.19724392727119	36.0	36.0	36.0	36.0	36.0
17	35.17266426544904	36.0	36.0	36.0	36.0	36.0
18	35.17130618526933	36.0	36.0	36.0	36.0	36.0
19	35.1691374954635	36.0	36.0	36.0	36.0	36.0
20	35.163330775527356	36.0	36.0	36.0	36.0	36.0
21	35.15923622559672	36.0	36.0	36.0	36.0	36.0
22	35.137498112551114	36.0	36.0	36.0	36.0	36.0
23	35.10257390631719	36.0	36.0	36.0	36.0	36.0
24	35.08928626617136	36.0	36.0	36.0	36.0	36.0
25	35.06910314610135	36.0	36.0	36.0	36.0	36.0
26	35.026621197176816	36.0	36.0	36.0	36.0	36.0
27	35.01740338248501	36.0	36.0	36.0	36.0	36.0
28	35.000470648072685	36.0	36.0	36.0	36.0	36.0
29	34.972969965942035	36.0	36.0	36.0	36.0	36.0
30	34.95388266999623	36.0	36.0	36.0	36.0	36.0
31	34.95575643211674	36.0	36.0	36.0	36.0	36.0
32	34.932375024393345	36.0	36.0	36.0	32.0	36.0
33	34.91082499397341	36.0	36.0	36.0	32.0	36.0
34	34.90002304674427	36.0	36.0	36.0	32.0	36.0
35	34.88279891671472	36.0	36.0	36.0	32.0	36.0
36	34.86356945502838	36.0	36.0	36.0	32.0	36.0
37	34.848516663855726	36.0	36.0	36.0	32.0	36.0
38	34.83039980361701	36.0	36.0	36.0	32.0	36.0
39	34.815055616827124	36.0	36.0	36.0	32.0	36.0
40	34.80445411446196	36.0	36.0	36.0	32.0	36.0
41	34.781374698560064	36.0	36.0	36.0	32.0	36.0
42	34.750220091992716	36.0	36.0	36.0	32.0	36.0
43	34.75666699926975	36.0	36.0	36.0	32.0	36.0
44	34.696540604213226	36.0	36.0	36.0	32.0	36.0
45	34.679021546498355	36.0	36.0	36.0	32.0	36.0
46	34.66024948762937	36.0	36.0	36.0	32.0	36.0
47	34.64147036462422	36.0	36.0	36.0	32.0	36.0
48	34.617552965568514	36.0	36.0	36.0	32.0	36.0
49	34.60301762237071	36.0	36.0	36.0	32.0	36.0
50	34.19088532169635	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	17.0
21	42.0
22	89.0
23	227.0
24	556.0
25	1261.0
26	2669.0
27	5165.0
28	8969.0
29	14512.0
30	21538.0
31	31928.0
32	48554.0
33	81964.0
34	186643.0
35	728345.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.67875080159489	17.867218788147525	12.113224946516175	26.34080546374141
2	16.109445575242727	20.04291637047601	36.891653825605886	26.955984228675376
3	18.23821898684214	23.423745605878786	28.647330325772046	29.69070508150703
4	12.52277080145274	16.008127288669744	35.654726216157265	35.814375693720244
5	14.776861401525853	36.440025430912684	33.19581802769144	15.587295139870019
6	34.78392554393896	35.23947442780446	16.451504662333992	13.525095365922576
7	30.767403603239256	30.221699083693238	20.266918385385715	18.74397892768179
8	28.263432234183178	32.997374790393835	19.125089074342085	19.614103901080902
9	27.35551413224593	14.122532740063631	18.663889283793726	39.858063843896716
10	16.250971319581804	26.629079542243574	31.1854513987002	25.934497739474427
11	37.44689756384434	21.113378502597392	22.07180517818842	19.367918755369846
12	24.50469367698001	23.5875921980148	28.569309330575965	23.33840479442922
13	29.272544086832365	19.580460952545785	25.080509076973478	26.066485883648376
14	23.65761544785299	19.571719084028782	24.978079102430858	31.79258636568737
15	25.2680618924291	27.18809410489006	21.96098654193757	25.58285746074327
16	25.83984119751765	25.943066343127803	23.487120256853125	24.729972202501422
17	24.155372143108803	26.073726623228115	24.99194246967499	24.778958763988093
18	25.00348793879595	25.0773969203708	25.790084363967736	24.129030776865516
19	25.761491804277508	24.96607007093276	25.243337415815365	24.029100708974365
20	25.87939742865216	24.388775077705567	24.805736083639445	24.926091410002826
21	26.654840125353097	24.332593659407973	24.47705524419394	24.53551097104499
22	26.008648268712676	24.368532452200082	24.971897983277426	24.65092129580982
23	24.453588584345862	24.371644532353773	25.404157247810115	25.77060963549025
24	24.83191607724708	24.92604660609111	25.27360548182292	24.968431834838892
25	24.987637750777054	24.324491381746256	25.431177592540266	25.25669327493642
26	24.753084621002003	25.21402276257359	25.527937531402518	24.504955085021894
27	25.238615381762536	24.8424021453971	24.910659963919606	25.00832250892076
28	24.770411316956007	24.661886512547902	25.531497801246843	25.036204369249244
29	24.710655864367865	24.920550122519263	25.277555795933687	25.091238217179185
30	24.593897244258578	25.047153483706442	25.664122380067678	24.694826891967306
31	25.2931406112442	24.79962065454751	24.660809888412743	25.246428845795542
32	25.15477190184667	24.77445378388712	24.66125020199157	25.40952411227464
33	24.70950896219709	24.514715063149072	25.135123918412617	25.64065205624122
34	25.16596348891546	24.77204855193655	25.45930252066707	24.602685438480922
35	25.911231829669095	24.401798533834654	25.265568072845568	24.421401563650686
36	24.661296147654834	25.051678662474092	25.124439393578157	25.162585796292912
37	25.749225806622523	24.91167528647583	24.729683549836682	24.60941535706496
38	24.959734388245266	24.693681124611473	25.234617829895452	25.11196665724781
39	25.47338918754724	24.612264144279063	24.70136126985921	25.212985398314487
40	25.702352359423564	24.696241876236225	25.206537863803334	24.394867900536873
41	24.73990389115435	25.11130576988625	25.378951774269602	24.7698385646898
42	25.715842448872184	25.29867794882935	24.993575631376352	23.991903970922117
43	25.306111636039315	24.274789611719928	25.365011523891102	25.05408722834965
44	24.806854597650908	24.968894516869373	24.942049756410288	25.28220112906943
45	25.097067531804683	25.165944197538277	24.90606723952037	24.83092103113667
46	24.814143381791308	25.044526998328415	24.963729371217855	25.177600248662422
47	25.035056178187816	24.79593242369762	25.33855261252415	24.83045878559041
48	25.803098833831083	25.28174026382298	24.396296093519577	24.51886480882636
49	25.03951679132485	25.36562966364367	24.62165431858923	24.97319922644225
50	25.191839344152278	25.474498833510822	24.411324746701425	24.922337075635475
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	341.0
1	189.0
2	37.0
3	114.5
4	192.0
5	182.5
6	173.0
7	127.0
8	81.0
9	139.0
10	197.0
11	319.5
12	442.0
13	880.0
14	1318.0
15	1622.0
16	1926.0
17	2036.0
18	2146.0
19	2107.5
20	2069.0
21	2573.0
22	3077.0
23	3787.5
24	4498.0
25	5740.5
26	6983.0
27	8753.0
28	10523.0
29	12348.5
30	14174.0
31	16176.0
32	18178.0
33	20965.0
34	23752.0
35	27270.5
36	30789.0
37	36363.0
38	41937.0
39	45763.5
40	49590.0
41	53994.0
42	58398.0
43	60095.0
44	61792.0
45	67121.0
46	72450.0
47	77728.0
48	83006.0
49	85581.5
50	88157.0
51	85930.0
52	83703.0
53	82621.0
54	81539.0
55	82667.5
56	83796.0
57	81708.0
58	79620.0
59	71988.5
60	64357.0
61	56625.5
62	48894.0
63	42247.0
64	35600.0
65	29902.5
66	24205.0
67	20930.0
68	17655.0
69	15825.0
70	13995.0
71	11015.0
72	8035.0
73	7095.0
74	6155.0
75	4463.5
76	2772.0
77	2511.0
78	2250.0
79	1910.0
80	1570.0
81	1252.0
82	934.0
83	788.0
84	642.0
85	486.0
86	330.0
87	226.5
88	123.0
89	73.5
90	24.0
91	19.0
92	14.0
93	14.0
94	14.0
95	14.0
96	14.0
97	12.5
98	11.0
99	7.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03205351789566448
2	0.004061878300828005
3	1.7660340438382631E-4
4	0.0
5	8.830170219191316E-5
6	8.830170219191316E-5
7	0.0
8	0.0
9	0.0
10	8.830170219191316E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.4150851095956574E-4
17	0.0
18	6.181119153433921E-4
19	0.0
20	8.830170219191316E-5
21	0.0
22	0.0
23	8.830170219191316E-5
24	9.713187241110448E-4
25	8.830170219191316E-5
26	7.064136175353053E-4
27	8.830170219191315E-4
28	0.0018543357460301761
29	5.298102131514789E-4
30	7.947153197272183E-4
31	3.5320680876765263E-4
32	7.064136175353053E-4
33	1.7660340438382631E-4
34	2.6490510657573946E-4
35	2.6490510657573946E-4
36	1.7660340438382631E-4
37	0.0010596204263029578
38	8.830170219191316E-5
39	7.947153197272183E-4
40	8.830170219191316E-5
41	0.0013245255328786973
42	0.0065343259622015724
43	0.004503386811787571
44	0.003885274896444179
45	0.0019426374482220895
46	0.0019426374482220895
47	0.001147922128494871
48	0.004856593620555224
49	0.004503386811787571
50	0.0020309391504140027
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1132481.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.0288100413711
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66270005367416	45.6470287875601
2	13.293563111125287	15.428192970969349
3	3.7525334845395117	6.53265158244683
4	1.4618315598585014	3.3931338359804073
5	0.7568857724067395	2.1960590355003564
6	0.45052630557072754	1.5686103242762677
7	0.28384897244388735	1.1529992671669305
8	0.20684276480667008	0.9602271605918606
9	0.15429102443653053	0.805799209330438
>10	0.8301936070482077	8.75073184053021
>50	0.07578449949276067	3.083574336740581
>100	0.06380715450322937	7.391097973870211
>500	0.006120587313881914	2.368453802923257
>1k	0.0010711027799293346	0.7214398721133487
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	1293	0.11417410093414371	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	1232	0.108787697100437	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	1231	0.10869939539824508	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.4150851095956574E-4
2	0.0	0.0	0.0	0.0	4.4150851095956574E-4
3	0.0	0.0	0.0	0.0	4.4150851095956574E-4
4	0.0	0.0	0.0	0.0	4.4150851095956574E-4
5	0.0	0.0	0.0	0.0	4.4150851095956574E-4
6	0.0	0.0	0.0	0.0	4.4150851095956574E-4
7	0.0	0.0	0.0	0.0	4.4150851095956574E-4
8	0.0	0.0	0.0	0.0	4.4150851095956574E-4
9	0.0	0.0	0.0	0.0	4.4150851095956574E-4
10	0.0	0.0	0.0	8.830170219191316E-5	4.4150851095956574E-4
11	0.0	0.0	0.0	8.830170219191316E-5	4.4150851095956574E-4
12	0.0	0.0	0.0	8.830170219191316E-5	6.181119153433921E-4
13	0.0	0.0	0.0	8.830170219191316E-5	6.181119153433921E-4
14	0.0	0.0	0.0	8.830170219191316E-5	6.181119153433921E-4
15	0.0	0.0	0.0	1.7660340438382631E-4	6.181119153433921E-4
16	0.0	0.0	0.0	2.6490510657573946E-4	6.181119153433921E-4
17	0.0	0.0	0.0	2.6490510657573946E-4	6.181119153433921E-4
18	0.0	0.0	0.0	2.6490510657573946E-4	7.947153197272184E-4
19	0.0	0.0	0.0	2.6490510657573946E-4	7.947153197272184E-4
20	0.0	0.0	0.0	4.4150851095956574E-4	8.830170219191315E-4
21	0.0	0.0	0.0	4.4150851095956574E-4	8.830170219191315E-4
22	0.0	0.0	0.0	5.298102131514789E-4	8.830170219191315E-4
23	0.0	0.0	0.0	7.064136175353053E-4	8.830170219191315E-4
24	0.0	0.0	0.0	9.713187241110447E-4	9.713187241110447E-4
25	0.0	0.0	0.0	0.0012362238306867842	9.713187241110447E-4
26	0.0	0.0	0.0	0.00167773234164635	9.713187241110447E-4
27	0.0	0.0	0.0	0.0021192408526059157	0.0010596204263029578
28	0.0	8.830170219191316E-5	0.0	0.0032671629811007867	0.0010596204263029578
29	0.0	8.830170219191316E-5	0.0	0.004150180003019918	0.0010596204263029578
30	0.0	1.7660340438382631E-4	0.0	0.005739610642474355	0.0010596204263029578
31	0.0	1.7660340438382631E-4	0.0	0.010154695752070012	0.0010596204263029578
32	0.0	1.7660340438382631E-4	0.0	0.014393177457281844	0.0010596204263029578
33	0.0	1.7660340438382631E-4	0.0	0.019956184695372373	0.0010596204263029578
34	0.0	1.7660340438382631E-4	0.0	0.025607493635654816	0.0010596204263029578
35	0.0	1.7660340438382631E-4	0.0	0.03143540598032108	0.0010596204263029578
36	0.0	1.7660340438382631E-4	0.0	0.03955916258197709	0.0010596204263029578
37	0.0	1.7660340438382631E-4	0.0	0.05147989237788537	0.0010596204263029578
38	0.0	1.7660340438382631E-4	0.0	0.06799231068777313	0.0010596204263029578
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	1395	0.0	26.980303	1
CGAATGC	315	0.0	20.95196	43
CTAGCGG	340	0.0	20.70638	29
AATACGA	330	0.0	20.66625	39
TAGGACG	140	1.8553692E-10	20.428162	4
GGTATCA	655	0.0	20.162058	1
TAGCGGC	350	0.0	20.114769	30
CGCAATA	350	0.0	20.113882	36
CAATACG	345	0.0	19.76772	38
GCGCAAT	360	0.0	19.555164	35
TCTAGCG	350	0.0	19.486183	28
CGGATCG	80	8.986273E-5	19.250463	26
TCTAGAT	290	0.0	18.966812	2
AACGCAG	2020	0.0	18.732298	6
TACGAAT	365	0.0	18.684555	41
ATACGAA	365	0.0	18.684555	40
TTAGGAC	225	0.0	18.577404	3
TCAACGC	2035	0.0	18.486115	4
CAACGCA	2090	0.0	18.210161	5
TATACTG	135	5.525908E-8	17.925568	5
>>END_MODULE