FastQCFastQC Report
Thu 2 Feb 2017
SRR4062105_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062105_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences4095
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTACAGGACATTGCGAGCAGATGGG461.1233211233211233No Hit
GGGTAGGGACGCTCTCCTGAGCTAC421.0256410256410255No Hit
GTCTTGGCAGTGCAGATAAAAAACT390.9523809523809524No Hit
CCCTTGGGCCGCGTCTCCTTCGAGT350.8547008547008548No Hit
GTCCACAGTCGGAAATGGTGATCTT330.805860805860806No Hit
GTATCAACGCAGAGTACATGGGGCT300.7326007326007326No Hit
CCATAATGGCACTGGCGGCAGGTCC240.5860805860805861No Hit
GTTCATGCCTTCTTTCACCTTCCCA230.5616605616605617No Hit
TATCAACGCAGAGTACATGGGGCTT220.5372405372405372No Hit
GGCTATAAGGGTTCCTCCTTTCACA220.5372405372405372No Hit
GCATACAGGTCCTGGCATCTTGTCC210.5128205128205128No Hit
GCCAAGACTGAATGGCTGGATGGCA210.5128205128205128No Hit
GTACATGGGGCTTTGCAGACGCCAC210.5128205128205128No Hit
CTCCTGAGCTACAGAAGGAATGGTT210.5128205128205128No Hit
CTGCCAAGACTGAATGGCTGGATGG190.463980463980464No Hit
GTCCAGCATTTGCCATGGACAAGAT180.43956043956043955No Hit
GGTGAAAGAAGGCATGAACATTGTG180.43956043956043955No Hit
GTGCCAGGGTGGTGACTTTACACGC170.41514041514041516No Hit
GTCTTTGGGAAGGTGAAAGAAGGCA170.41514041514041516No Hit
CCGTAGATGGACCTGCCGCCAGTGC160.3907203907203907No Hit
ATCATAAACTTAACTTTGCAATCCA160.3907203907203907No Hit
GTGGAAGCCATGGAGCGTTTTGGGT150.3663003663003663No Hit
GTTCCAAAGACAGCAGAAAACTTTC150.3663003663003663No Hit
GAATTATTCCAGGATTCATGTGCCA140.3418803418803419No Hit
GTCGGAAATGGTGATCTTCTTGCTG140.3418803418803419No Hit
GACCTGTATGCTTTAGGATGAAGTT140.3418803418803419No Hit
AATTAGAGCTGTCCACAGTCGGAAA140.3418803418803419No Hit
CCCAAAGACCACATGCTTGCCATCC140.3418803418803419No Hit
GATAAAAAACTGGGAACCGTTTGTG140.3418803418803419No Hit
GCCATGGTCAACCCCACCGTGTTCT130.31746031746031744No Hit
ACGCAGAGTACATGGGGCTTTGCAG130.31746031746031744No Hit
CCATTATGGCGTGTAAAGTCACCAC130.31746031746031744No Hit
GCCGATGACGAGCCCTTGGGCCGCG130.31746031746031744No Hit
ACCCCACCGTGTTCTTCGACATCAC130.31746031746031744No Hit
TTCCTGGACCCAAAACGCTCCATGG130.31746031746031744No Hit
CTATAAGGGTTCCTCCTTTCACAGA130.31746031746031744No Hit
GTGTAAAGTCACCACCCTGGCACAT120.29304029304029305No Hit
ATGCTGGACCAAACACAAACGGTTC120.29304029304029305No Hit
CCTGTATGCTTTAGGATGAAGTTCT120.29304029304029305No Hit
TAGATGGACCTGCCGCCAGTGCCAT120.29304029304029305No Hit
CTCCATGGCTTCCACAATGTTCATG120.29304029304029305No Hit
GTTTTTATTCATAATCATAAACTTA110.2686202686202686No Hit
GTTTTTTATCTGCACTGCCAAGACT110.2686202686202686No Hit
GGAGAGAAAGGATTTGGCTATAAGG110.2686202686202686No Hit
AAACTTAACTTTGCAATCCAGCTAG110.2686202686202686No Hit
GTCCATCTACGGAGAGAAATTTGAG110.2686202686202686No Hit
CTTATAGCCAAATCCTTTCTCTCCA110.2686202686202686No Hit
AAAGCATACAGGTCCTGGCATCTTG110.2686202686202686No Hit
GAATAATTCTGTGAAAGGAGGAACC110.2686202686202686No Hit
GGCAAATGCTGGACCAAACACAAAC110.2686202686202686No Hit
ATATGGAACCCAAAGAACTTCAGTG100.2442002442002442No Hit
GTGCAGATAAAAAACTGGGAACCGT100.2442002442002442No Hit
ACATTGTGGAAGCCATGGAGCGTTT100.2442002442002442No Hit
GGGGGGGGGGGGGGGGGGGGGGGGG100.2442002442002442No Hit
TGCCATTCCTGGACCCAAAACGCTC100.2442002442002442No Hit
ACTGTGGACAGCTCTAATTTCTTTT100.2442002442002442No Hit
CCCTTATAGCCAAATCCTTTCTCTC100.2442002442002442No Hit
CGCCATAATGGCACTGGCGGCAGGT100.2442002442002442No Hit
TGGCAGTGCAGATAAAAAACTGGGA100.2442002442002442No Hit
TAATGGCACTGGCGGCAGGTCCATC100.2442002442002442No Hit
TCCCTACCCCATCTGCTCGCAATGT100.2442002442002442No Hit
GGTCAACCCCACCGTGTTCTTCGAC100.2442002442002442No Hit
GACTTGAAGGGGAATGAGGAAAATA100.2442002442002442No Hit
ATACAGGTCCTGGCATCTTGTCCAT90.21978021978021978No Hit
CTTTACACGCCATAATGGCACTGGC90.21978021978021978No Hit
CCACAATGTTCATGCCTTCTTTCAC90.21978021978021978No Hit
CTCCAGTGCTCAGAGCTCGAAAGTT90.21978021978021978No Hit
GACCACATGCTTGCCATCCAGCCAT90.21978021978021978No Hit
GCATTTGCCATGGACAAGATGCCAG90.21978021978021978No Hit
GTCACCACCCTGGCACATGAATCCT90.21978021978021978No Hit
GATGAGAACTTCATCCTAAAGCATA90.21978021978021978No Hit
CCCTGGCACATGAATCCTGGAATAA90.21978021978021978No Hit
GGTATCAACGCAGAGTACATGGGGC90.21978021978021978No Hit
TCTTTCACCTTCCCAAAGACCACAT90.21978021978021978No Hit
GATGGGGTAGGGACGCTCTCCTGAG90.21978021978021978No Hit
GATTACAGGACATTGCGAGCAGATG90.21978021978021978No Hit
CATCTACGGAGAGAAATTTGAGGAT80.19536019536019536No Hit
GTGAAAGGAGGAACCCTTATAGCCA80.19536019536019536No Hit
ACTTCAGTGAGAGCAGAGATTACAG80.19536019536019536No Hit
AGCTAGACTTGAAGGGGAATGAGGA80.19536019536019536No Hit
GCACTGCCAAGACTGAATGGCTGGA80.19536019536019536No Hit
CCTTCGAGTTGCTTGCAGACAAAGT80.19536019536019536No Hit
TTCTTTCACCTTCCCAAAGACCACA80.19536019536019536No Hit
TCCTAAAGCATACAGGTCCTGGCAT80.19536019536019536No Hit
GTTTTGGGTCCAGGAATGGCAAGAC80.19536019536019536No Hit
GGCATGAACATTGTGGAAGCCATGG80.19536019536019536No Hit
GTATGCTTTAGGATGAAGTTCTCAT80.19536019536019536No Hit
ACTTTACACGCCATAATGGCACTGG80.19536019536019536No Hit
CTTGGGCCGCGTCTCCTTCGAGTTG80.19536019536019536No Hit
ACAGAAGGAATGGTTTGATGGGTAA70.17094017094017094No Hit
GCTATAAGGGTTCCTCCTTTCACAG70.17094017094017094No Hit
CTTCCACAATGTTCATGCCTTCTTT70.17094017094017094No Hit
CCATCCAGCCATTCAGTCTTGGCAG70.17094017094017094No Hit
CTACAGAAGGAATGGTTTGATGGGT70.17094017094017094No Hit
ATTTCCGACTGTGGACAGCTCTAAT70.17094017094017094No Hit
ATTCAGTCTTGGCAGTGCAGATAAA70.17094017094017094No Hit
GAGGAACCCTTATAGCCAAATCCTT70.17094017094017094No Hit
GACCAGCAAGAAGATCACCATTTCC70.17094017094017094No Hit
ATCCTAAAGCATACAGGTCCTGGCA70.17094017094017094No Hit
ATCCTGGAATAATTCTGTGAAAGGA70.17094017094017094No Hit
GAGCTACAGAAGGAATGGTTTGATG70.17094017094017094No Hit
CTTGCCATCCAGCCATTCAGTCTTG70.17094017094017094No Hit
GCCCAAGGGCTCGTCATCGGCCGTG70.17094017094017094No Hit
GCCATGGAGCGTTTTGGGTCCAGGA70.17094017094017094No Hit
GGGTAAAATGCCCGCAAGTCAAAAG70.17094017094017094No Hit
GTATCAACGCAGAGTACTTTTTTTT70.17094017094017094No Hit
GGAAAATATGGAACCCAAAGAACTT70.17094017094017094No Hit
GGTATCAACGCAGAGTACTTTTTTT70.17094017094017094No Hit
GCTCTGAGCACTGGAGAGAAAGGAT70.17094017094017094No Hit
CTGCAGCCATGGTCAACCCCACCGT60.14652014652014653No Hit
TATCTGCACTGCCAAGACTGAATGG60.14652014652014653No Hit
TCTTGCTGGTCTTGCCATTCCTGGA60.14652014652014653No Hit
GAGGAAAATATGGAACCCAAAGAAC60.14652014652014653No Hit
GGATTCATGTGCCAGGGTGGTGACT60.14652014652014653No Hit
CTTGCAGACAAAGTTCCAAAGACAG60.14652014652014653No Hit
GGTAAAATGCCCGCAAGTCAAAAGA60.14652014652014653No Hit
GTGGTGACTTTACACGCCATAATGG60.14652014652014653No Hit
GGGTTCCTCCTTTCACAGAATTATT60.14652014652014653No Hit
GTCCATGGCAAATGCTGGACCAAAC60.14652014652014653No Hit
ACCAAACACAAACGGTTCCCAGTTT60.14652014652014653No Hit
ACCCAAAGAACTTCAGTGAGAGCAG60.14652014652014653No Hit
ATTCATAATCATAAACTTAACTTTG60.14652014652014653No Hit
CATGAACATTGTGGAAGCCATGGAG60.14652014652014653No Hit
CTTTGGAACTTTGTCTGCAAGCAAC60.14652014652014653No Hit
GCTGTCCACAGTCGGAAATGGTGAT60.14652014652014653No Hit
GACTTTACACGCCATAATGGCACTG60.14652014652014653No Hit
GCAGACGCCACTGTCGCTTTTCGCC60.14652014652014653No Hit
CAACCCCACCGTGTTCTTCGACATC60.14652014652014653No Hit
AACACAAACGGTTCCCAGTTTTTTA60.14652014652014653No Hit
GCTTTGCAGACGCCACTGTCGCTTT60.14652014652014653No Hit
GTCCTGGCATCTTGTCCATGGCAAA60.14652014652014653No Hit
TCATGTGCCAGGGTGGTGACTTTAC60.14652014652014653No Hit
ATCTTGTCCATGGCAAATGCTGGAC60.14652014652014653No Hit
GGTTTGATGGGTAAAATGCCCGCAA60.14652014652014653No Hit
TTCCAGGATTCATGTGCCAGGGTGG60.14652014652014653No Hit
GAGCAGAGATTACAGGACATTGCGA60.14652014652014653No Hit
CCACTGTCGCTTTTCGCCGCTTGCT50.1221001221001221No Hit
ACAATGTTCATGCCTTCTTTCACCT50.1221001221001221No Hit
GGACATTGCGAGCAGATGGGGTAGG50.1221001221001221No Hit
GGACAAGATGCCAGGACCTGTATGC50.1221001221001221No Hit
CGGAAATGGTGATCTTCTTGCTGGT50.1221001221001221No Hit
TCCTGGAATAATTCTGTGAAAGGAG50.1221001221001221No Hit
GCTTTAGGATGAAGTTCTCATCCTC50.1221001221001221No Hit
GAGTACATGGGGCTTTGCAGACGCC50.1221001221001221No Hit
GTGTTCTTCGACATCACGGCCGATG50.1221001221001221No Hit
TCTTCTTGCTGGTCTTGCCATTCCT50.1221001221001221No Hit
GGGTGGTGACTTTACACGCCATAAT50.1221001221001221No Hit
GGCTTTGCAGACGCCACTGTCGCTT50.1221001221001221No Hit
TCTCTCCAGTGCTCAGAGCTCGAAA50.1221001221001221No Hit
GCACTGGAGAGAAAGGATTTGGCTA50.1221001221001221No Hit
TCCAAAGACAGCAGAAAACTTTCGA50.1221001221001221No Hit
GGTCTTGCCATTCCTGGACCCAAAA50.1221001221001221No Hit
GACGAGCCCTTGGGCCGCGTCTCCT50.1221001221001221No Hit
GGTCCATCTACGGAGAGAAATTTGA50.1221001221001221No Hit
TTTCTCTCCAGTGCTCAGAGCTCGA50.1221001221001221No Hit
TATCAACGCAGAGTACTTTTTTTTT50.1221001221001221No Hit
GACTGAATGGCTGGATGGCAAGCAT50.1221001221001221No Hit
CGTCTCCTTCGAGTTGCTTGCAGAC50.1221001221001221No Hit
TGTTCATGCCTTCTTTCACCTTCCC50.1221001221001221No Hit
ACATGGGGCTTTGCAGACGCCACTG50.1221001221001221No Hit
TTGCAATCCAGCTAGACTTGAAGGG50.1221001221001221No Hit
ACTGGGAACCGTTTGTGTTTGGTCC50.1221001221001221No Hit
ACCCAAAACGCTCCATGGCTTCCAC50.1221001221001221No Hit
TGCCAGGGTGGTGACTTTACACGCC50.1221001221001221No Hit
ATTCATGTGCCAGGGTGGTGACTTT50.1221001221001221No Hit
GCTCAGAGCTCGAAAGTTTTCTGCT50.1221001221001221No Hit

[OK]Adapter Content

Adapter graph

[OK]Kmer Content

No overrepresented Kmers