FastQCFastQC Report
Thu 2 Feb 2017
SRR4062104_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062104_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320942
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT23220.1757836453076668No Hit
TATCAACGCAGAGTACTTTTTTTTT14710.11135992344856929No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA6450.016.0522461
TACTCTA508.7076056E-515.2009715
CGCGTAA1006.5847416E-1014.25145110
CCCGTTA400.00527630814.250918
TTATACT759.666692E-713.9336954
GCGTAAC1153.765308E-1013.21873611
AAGACGG1950.012.6674765
CTATATG604.0943286E-412.6655542
GCGTTAT853.94758E-612.2925731
CGAGCCG2100.012.21367615
CCGGTCG550.003065684812.092149
TAGTACT550.003067426212.0912234
CGTTATT951.0404692E-611.9989462
TCGCGTA1209.98989E-911.8762089
GTCCTAT2250.011.8207371
CGTAACT1302.6102498E-911.69305412
TACGCTA907.4496093E-611.6122939
ATACGCT907.4527543E-611.6118538
GTATTAC907.468496E-611.60965251
CTATAAT1157.081144E-811.5637631
Produced by FastQC (version 0.11.5)