##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062104_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1320942
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.285888403881472	32.0	32.0	32.0	32.0	32.0
2	30.825199743819184	32.0	32.0	32.0	32.0	32.0
3	30.838669676639853	32.0	32.0	32.0	32.0	32.0
4	30.837465233144226	32.0	32.0	32.0	32.0	32.0
5	30.745798831439988	32.0	32.0	32.0	32.0	32.0
6	34.44329879737339	36.0	36.0	36.0	32.0	36.0
7	34.36768457661275	36.0	36.0	36.0	32.0	36.0
8	34.32520731417428	36.0	36.0	36.0	32.0	36.0
9	34.466865312784364	36.0	36.0	36.0	32.0	36.0
10	34.134139878965165	36.0	36.0	36.0	32.0	36.0
11	34.45553173417152	36.0	36.0	36.0	32.0	36.0
12	34.26702686416209	36.0	36.0	36.0	32.0	36.0
13	34.34833929120279	36.0	36.0	36.0	32.0	36.0
14	34.244814685277625	36.0	36.0	36.0	32.0	36.0
15	34.19603888740005	36.0	36.0	36.0	32.0	36.0
16	34.208869882250696	36.0	36.0	36.0	32.0	36.0
17	34.13848677686075	36.0	36.0	36.0	32.0	36.0
18	34.14012045949027	36.0	36.0	36.0	32.0	36.0
19	34.151883277237005	36.0	36.0	36.0	32.0	36.0
20	34.13664490946612	36.0	36.0	36.0	32.0	36.0
21	34.12016803160169	36.0	36.0	36.0	32.0	36.0
22	34.09391101198993	36.0	36.0	36.0	32.0	36.0
23	34.05859227732936	36.0	36.0	36.0	32.0	36.0
24	34.03314679978379	36.0	36.0	36.0	32.0	36.0
25	33.685148174560275	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	23.0
5	117.0
6	298.0
7	55.0
8	231.0
9	215.0
10	119.0
11	47.0
12	39.0
13	54.0
14	196.0
15	324.0
16	450.0
17	637.0
18	829.0
19	1121.0
20	1644.0
21	2378.0
22	3702.0
23	5446.0
24	7728.0
25	10786.0
26	15001.0
27	19354.0
28	25616.0
29	34122.0
30	44407.0
31	61027.0
32	85618.0
33	122698.0
34	270717.0
35	605942.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.39597799129705	17.305054310138598	11.73860577228805	25.560361926276297
2	16.979562296877756	19.15293718644512	37.30923637013311	26.55826414654402
3	19.432068951159597	22.71542505876519	27.91280418794263	29.93970180213258
4	13.217171147420837	15.162890285183868	34.47554771853661	37.144390848858684
5	15.403781083676392	35.78905357797387	33.01852070629504	15.788644632054696
6	35.286083210295345	34.370589665717965	16.242908428676728	14.10041869530996
7	31.04363247766081	29.934820339085817	19.957633220405782	19.063913962847593
8	28.382400534796243	33.1738140840288	18.643818578560705	19.799966802614254
9	26.850281136917996	14.907609115839932	18.810856781622785	39.43125296561929
10	16.772917941264993	26.860079618329348	30.456929758923707	25.910072681481957
11	36.75944226979907	21.849613605572145	21.652796194661214	19.73814792996757
12	24.421108173018606	23.79074076320322	28.360165328352053	23.42798573542612
13	29.149821816673953	20.050002008873914	24.98584312438265	25.814333050069475
14	23.557376726107368	19.52455017744285	24.943795867196037	31.97427722925374
15	25.293593665305874	27.06808128361705	21.916404174105537	25.721920876971538
16	25.861134545664026	26.304407641458162	23.119329754100164	24.71512805877765
17	24.13743961224485	26.32265576496968	24.821423562917353	24.718481059868118
18	24.609849305952423	25.671119829129534	25.681352047428984	24.03767881748906
19	25.46847587615428	25.154807149434085	25.201868825996836	24.174848148414792
20	25.852669707946223	24.639138539882694	24.894742425849866	24.613449326321213
21	26.38704177323103	24.486350288907836	24.573117362887185	24.553490574973953
22	25.952967942747275	24.391295681717764	25.071183183110357	24.584553192424607
23	24.71847282018176	24.659750289066395	25.34078010798884	25.280996782763005
24	24.596003876847206	24.975428700036144	25.31688830724393	25.11167911587272
25	24.99158876805005	24.65354817589932	25.07858060839729	25.276282447653344
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	128.0
1	128.0
2	280.0
3	432.0
4	432.0
5	432.0
6	904.0
7	1376.0
8	1376.0
9	1376.0
10	1675.0
11	1974.0
12	1974.0
13	1974.0
14	2776.5
15	3579.0
16	3579.0
17	3579.0
18	6017.0
19	8455.0
20	8455.0
21	8455.0
22	12950.5
23	17446.0
24	17446.0
25	17446.0
26	25183.0
27	32920.0
28	32920.0
29	32920.0
30	42351.5
31	51783.0
32	51783.0
33	51783.0
34	64491.5
35	77200.0
36	77200.0
37	77200.0
38	92192.0
39	107184.0
40	107184.0
41	107184.0
42	123266.5
43	139349.0
44	139349.0
45	139349.0
46	154523.0
47	169697.0
48	169697.0
49	169697.0
50	175989.0
51	182281.0
52	182281.0
53	182281.0
54	172906.0
55	163531.0
56	163531.0
57	163531.0
58	150607.0
59	137683.0
60	137683.0
61	137683.0
62	119737.5
63	101792.0
64	101792.0
65	101792.0
66	82432.5
67	63073.0
68	63073.0
69	63073.0
70	47494.0
71	31915.0
72	31915.0
73	31915.0
74	23759.0
75	15603.0
76	15603.0
77	15603.0
78	11913.0
79	8223.0
80	8223.0
81	8223.0
82	5618.5
83	3014.0
84	3014.0
85	3014.0
86	2141.0
87	1268.0
88	1268.0
89	1268.0
90	843.5
91	419.0
92	419.0
93	419.0
94	266.0
95	113.0
96	113.0
97	113.0
98	308.5
99	504.0
100	504.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	7.570355095076089E-4
2	7.570355095076089E-5
3	5.299248566553263E-4
4	0.010977014887860329
5	0.035883483150660665
6	0.06730045679522643
7	0.11446376903755048
8	0.11817324303413776
9	0.12536508037446004
10	0.14376104325549496
11	0.14784903500683602
12	0.14156564027792287
13	0.1358878739566158
14	0.12445663776305092
15	0.14058149411556298
16	0.1260464123330169
17	0.1331625461223884
18	0.11961161050220222
19	0.10575786067821297
20	0.1000800943569059
21	0.09970157660215209
22	0.11045148083716014
23	0.08902737591809481
24	0.09939876239834905
25	0.09674913811507242
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1320942.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	56.94416031611531
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.93346481068004	43.80911553853579
2	13.326421270890268	15.17723738579329
3	4.125111527199301	7.047030363800766
4	1.8156501038440753	4.135626823650737
5	1.001101857581716	2.850345233544704
6	0.6132299101379945	2.095191738812097
7	0.4053427269920159	1.61573308601633
8	0.3037197776760686	1.383605416892876
9	0.22629518008057264	1.1597570111945081
>10	1.1386191349379344	11.502892210005198
>50	0.06265394474305239	2.4892532066670054
>100	0.04452392890575998	5.012215269008643
>500	0.0034659132626027084	1.3574814755840363
>1k	3.99913068761851E-4	0.364515240494019
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2322	0.1757836453076668	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1471	0.11135992344856929	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	7.57035509507609E-5	0.0
5	0.0	0.0	0.0	7.57035509507609E-5	0.0
6	0.0	0.0	0.0	7.57035509507609E-5	0.0
7	0.0	0.0	0.0	1.514071019015218E-4	0.0
8	0.0	0.0	0.0	1.514071019015218E-4	0.0
9	0.0	0.0	0.0	1.514071019015218E-4	0.0
10	0.0	0.0	0.0	1.514071019015218E-4	0.0
11	0.0	0.0	0.0	1.514071019015218E-4	0.0
12	0.0	0.0	0.0	1.514071019015218E-4	3.028142038030436E-4
13	0.0	0.0	0.0	1.514071019015218E-4	3.028142038030436E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	645	0.0	16.052246	1
TACTCTA	50	8.7076056E-5	15.200971	5
CGCGTAA	100	6.5847416E-10	14.251451	10
CCCGTTA	40	0.005276308	14.25091	8
TTATACT	75	9.666692E-7	13.933695	4
GCGTAAC	115	3.765308E-10	13.218736	11
AAGACGG	195	0.0	12.667476	5
CTATATG	60	4.0943286E-4	12.665554	2
GCGTTAT	85	3.94758E-6	12.292573	1
CGAGCCG	210	0.0	12.213676	15
CCGGTCG	55	0.0030656848	12.09214	9
TAGTACT	55	0.0030674262	12.091223	4
CGTTATT	95	1.0404692E-6	11.998946	2
TCGCGTA	120	9.98989E-9	11.876208	9
GTCCTAT	225	0.0	11.820737	1
CGTAACT	130	2.6102498E-9	11.693054	12
TACGCTA	90	7.4496093E-6	11.612293	9
ATACGCT	90	7.4527543E-6	11.611853	8
GTATTAC	90	7.468496E-6	11.6096525	1
CTATAAT	115	7.081144E-8	11.563763	1
>>END_MODULE