FastQCFastQC Report
Thu 2 Feb 2017
SRR4062104_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062104_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1320942
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5500.032.008621
GTATCAA16550.029.1196251
AGCGACG500.002581066721.9992633
CGACGTT654.9336127E-420.307017
ATACCGT2850.019.2975986
TCAACGC26150.018.5079824
TATCAAC26350.018.3675062
AACGTCG600.007410655318.33410622
CGCACGA600.00741389218.33271810
GGCGTTA3400.018.11909742
TACGCTC851.4313275E-418.1170394
ATCAACG26600.018.1121753
CAACGCA26950.017.9585825
AACGCAG27950.017.4734756
AGCGTAT1408.376992E-817.2851358
CGTCGTA2800.017.28513510
CGGCGTT3950.016.70956441
ACCGTCG2900.016.6890958
TACCGTC2900.016.6890957
ACGGACC4250.016.564158
Produced by FastQC (version 0.11.5)