##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062104_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1320942
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.262037243118925	32.0	32.0	32.0	32.0	32.0
2	31.492745328712388	32.0	32.0	32.0	32.0	32.0
3	31.602048386681627	32.0	32.0	32.0	32.0	32.0
4	31.67994128432588	32.0	32.0	32.0	32.0	32.0
5	31.654640400562627	32.0	32.0	32.0	32.0	32.0
6	35.315602047629646	36.0	36.0	36.0	36.0	36.0
7	35.32656467884283	36.0	36.0	36.0	36.0	36.0
8	35.281885957142705	36.0	36.0	36.0	36.0	36.0
9	35.36325061963356	36.0	36.0	36.0	36.0	36.0
10	35.24488736068654	36.0	36.0	36.0	36.0	36.0
11	35.364331666341144	36.0	36.0	36.0	36.0	36.0
12	35.289529744682206	36.0	36.0	36.0	36.0	36.0
13	35.3180268323666	36.0	36.0	36.0	36.0	36.0
14	35.28678700503126	36.0	36.0	36.0	36.0	36.0
15	35.26602227804097	36.0	36.0	36.0	36.0	36.0
16	35.27799479462384	36.0	36.0	36.0	36.0	36.0
17	35.25596279019064	36.0	36.0	36.0	36.0	36.0
18	35.255861347432365	36.0	36.0	36.0	36.0	36.0
19	35.24857412361784	36.0	36.0	36.0	36.0	36.0
20	35.24538094783874	36.0	36.0	36.0	36.0	36.0
21	35.23449553424753	36.0	36.0	36.0	36.0	36.0
22	35.22282507483296	36.0	36.0	36.0	36.0	36.0
23	35.19719336655205	36.0	36.0	36.0	36.0	36.0
24	35.176708742700285	36.0	36.0	36.0	36.0	36.0
25	35.16555155336116	36.0	36.0	36.0	36.0	36.0
26	35.12142546758298	36.0	36.0	36.0	36.0	36.0
27	35.115274554068236	36.0	36.0	36.0	36.0	36.0
28	35.100731901930594	36.0	36.0	36.0	36.0	36.0
29	35.08204977962696	36.0	36.0	36.0	36.0	36.0
30	35.06450850983616	36.0	36.0	36.0	36.0	36.0
31	35.061886895866735	36.0	36.0	36.0	36.0	36.0
32	35.045204861379226	36.0	36.0	36.0	36.0	36.0
33	35.025057118329194	36.0	36.0	36.0	36.0	36.0
34	35.003848011494824	36.0	36.0	36.0	36.0	36.0
35	34.99641922204003	36.0	36.0	36.0	36.0	36.0
36	34.980077853531796	36.0	36.0	36.0	36.0	36.0
37	34.96981245202287	36.0	36.0	36.0	36.0	36.0
38	34.958024652104335	36.0	36.0	36.0	36.0	36.0
39	34.9335852747509	36.0	36.0	36.0	36.0	36.0
40	34.91762393806844	36.0	36.0	36.0	36.0	36.0
41	34.8924199548504	36.0	36.0	36.0	36.0	36.0
42	34.870462896932644	36.0	36.0	36.0	36.0	36.0
43	34.857653099076266	36.0	36.0	36.0	32.0	36.0
44	34.81390628808835	36.0	36.0	36.0	32.0	36.0
45	34.80839885475668	36.0	36.0	36.0	32.0	36.0
46	34.790052856219276	36.0	36.0	36.0	32.0	36.0
47	34.7634839379776	36.0	36.0	36.0	32.0	36.0
48	34.74065553218839	36.0	36.0	36.0	32.0	36.0
49	34.717603043888374	36.0	36.0	36.0	32.0	36.0
50	34.32983204410186	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	10.0
21	22.0
22	81.0
23	240.0
24	636.0
25	1302.0
26	2943.0
27	5433.0
28	9329.0
29	15190.0
30	22266.0
31	33111.0
32	50236.0
33	85870.0
34	198430.0
35	895841.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.84710336993563	17.885346459674363	12.167285119273002	26.100265051117
2	16.372115559324087	19.58285385500575	37.14788625764642	26.897144328023742
3	18.83490191803388	23.062888739499872	28.23369186697917	29.868517475487078
4	12.93253289324269	15.544233651793421	34.64010477387315	36.88312868109074
5	15.018221844713848	36.185086097648494	32.892133038392295	15.904559019245356
6	35.38033521583115	34.43456932184657	16.237452117431523	13.947643344890759
7	31.040348478585738	29.97391255634237	19.930928080112526	19.05481088495937
8	28.490350068360303	33.086918274988605	18.63972831509635	19.78300334155474
9	26.90473919369662	14.762798063805981	18.687951477051985	39.644511265445416
10	16.68581966505721	26.948798660349958	30.524883000162006	25.840498674430822
11	37.01547834802739	21.72737334417408	21.602386781554376	19.65476152624415
12	24.504179593047994	23.721026358462368	28.3704356436543	23.40435840483534
13	29.27070227156075	19.853331940387996	25.115561470526337	25.760404317524916
14	23.64971361346675	19.34520970640649	24.91691535283154	32.08816132729522
15	25.155684352530237	27.119055946438227	22.00369130514436	25.72156839588718
16	25.93163458197287	26.164197827138587	23.192808708403394	24.711358882485158
17	24.16851004813232	26.18865930525337	24.896853911829588	24.745976734784723
18	24.68783546237598	25.54480225415576	25.71808820701653	24.049274076451734
19	25.454637675234792	25.031681936072893	25.313450552711625	24.200229835980686
20	25.88694414583554	24.5398731206565	24.926491740730086	24.64669099277787
21	26.479360940904296	24.312649609142568	24.752865757921242	24.4551236920319
22	25.921122956193383	24.191296816968496	25.226164358465397	24.66141586837272
23	24.81956058630886	24.442935420328826	25.52844863741179	25.209055355950525
24	24.77108948158534	24.829911630509088	25.3269095811969	25.072089306708676
25	25.078599390282214	24.541784291326092	25.183221935305113	25.196394383086577
26	24.87461551913528	25.16221439781004	25.39947022454515	24.563699858509526
27	25.132633223714095	24.73299236299109	25.25391086320609	24.880463550088724
28	24.895451971498737	24.6557712631461	25.538183877346466	24.910592888008697
29	24.776502668946872	24.87567499638513	25.53467889741569	24.813143437252304
30	24.867555033362958	25.07642366037159	25.460926652969206	24.595094653296247
31	25.302645932836864	24.665899027429855	24.935859729448808	25.095595310284473
32	25.05019168226167	24.824518371821195	24.951246691739797	25.17404325417734
33	24.826562902175723	24.7408663527488	25.195996790164582	25.236573954910895
34	25.27054658927716	24.626079820612187	25.322479421804374	24.780894168306286
35	25.398484867984262	24.552194231233642	25.503939994291947	24.54538090649015
36	24.74639272033552	25.076536405892774	25.113328387360518	25.063742486411194
37	25.414199010843173	24.65578992870894	25.163995176100347	24.766015884347535
38	24.672468586811533	24.81448844839516	25.372575026004167	25.140467938789136
39	25.38957460209792	24.541383027689626	24.966992901959834	25.102049468252623
40	25.361314396328073	24.730097710647183	25.26093141177388	24.647656481250866
41	24.77665407708037	25.19143665878587	25.253059769117737	24.778849495016022
42	25.413779058411322	25.13161739878293	25.141383659607197	24.31321988319855
43	25.164700057385726	24.56767654438006	25.179160048634024	25.088463349600193
44	24.725563408858285	24.82330068862765	25.14959633216442	25.301539570349647
45	24.969623375276417	24.944337910709	25.183489954327182	24.9025487596874
46	25.086247294480856	24.87124416406556	25.000927390263232	25.04158115119035
47	24.820145246482205	24.990177352722746	25.407384359620178	24.78229304117487
48	25.166401695813462	25.50177909001438	24.64789158906806	24.683927625104097
49	24.907562745288047	25.11658752846553	24.826405712486068	25.14944401376036
50	24.85544144299646	25.439126425713027	24.93319040243225	24.772241728858262
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	55.0
1	59.0
2	63.0
3	167.0
4	271.0
5	282.0
6	293.0
7	224.5
8	156.0
9	245.5
10	335.0
11	524.0
12	713.0
13	1304.5
14	1896.0
15	2293.0
16	2690.0
17	2632.0
18	2574.0
19	2466.5
20	2359.0
21	2830.0
22	3301.0
23	4182.5
24	5064.0
25	6422.5
26	7781.0
27	9549.5
28	11318.0
29	13235.5
30	15153.0
31	17678.5
32	20204.0
33	23339.0
34	26474.0
35	30691.5
36	34909.0
37	40356.0
38	45803.0
39	50932.5
40	56062.0
41	62047.0
42	68032.0
43	71145.0
44	74258.0
45	79606.0
46	84954.0
47	89565.0
48	94176.0
49	98778.5
50	103381.0
51	103356.5
52	103332.0
53	103320.5
54	103309.0
55	101616.5
56	99924.0
57	95343.0
58	90762.0
59	84572.0
60	78382.0
61	68081.5
62	57781.0
63	49948.5
64	42116.0
65	35577.5
66	29039.0
67	24410.0
68	19781.0
69	16777.5
70	13774.0
71	10554.5
72	7335.0
73	6485.5
74	5636.0
75	4196.5
76	2757.0
77	2363.0
78	1969.0
79	1583.5
80	1198.0
81	967.5
82	737.0
83	584.0
84	431.0
85	338.5
86	246.0
87	164.0
88	82.0
89	53.5
90	25.0
91	17.0
92	9.0
93	10.0
94	11.0
95	12.0
96	13.0
97	14.0
98	15.0
99	9.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03346096952023632
2	0.004693620158947176
3	4.542213057045654E-4
4	1.5140710190152178E-4
5	0.0
6	1.5140710190152178E-4
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	8.327390604583698E-4
17	0.0
18	3.0281420380304355E-4
19	0.0
20	1.5140710190152178E-4
21	0.0
22	0.0
23	0.0
24	8.327390604583698E-4
25	2.271106528522827E-4
26	3.7851775475380447E-4
27	4.542213057045654E-4
28	0.0013626639171136963
29	6.813319585568481E-4
30	0.0012112568152121742
31	6.813319585568481E-4
32	4.542213057045654E-4
33	1.5140710190152178E-4
34	3.7851775475380447E-4
35	7.570355095076089E-5
36	1.5140710190152178E-4
37	0.001438367468064457
38	0.0
39	0.0010598497133106526
40	7.570355095076089E-5
41	6.813319585568481E-4
42	0.005147841464651741
43	0.00423939885324261
44	0.004087991751341088
45	0.0018925887737690225
46	0.002346810079473588
47	0.0011355532642614134
48	0.0031795491399319573
49	0.0034823633437350014
50	0.0018168852228182615
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1320942.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	58.31527978925347
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72175567385142	44.740506480433915
2	13.690971178237044	15.967856296909972
3	4.211249290572475	7.367405419260872
4	1.7648827758322803	4.11678531471575
5	0.9449560551886135	2.7552688373436625
6	0.5848777343999417	2.0464385234422373
7	0.38788191941562966	1.583360985914058
8	0.28808240873844976	1.343968501435582
9	0.2123406346385666	1.114443316761809
>10	1.0949863965983375	11.145441442730808
>50	0.05737872411455059	2.301175447227455
>100	0.037109248191751015	4.0670772183525985
>500	0.003397295027718845	1.3645497207303428
>1k	1.3066519337380172E-4	0.08572249474098925
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	7.57035509507609E-5
2	0.0	0.0	0.0	0.0	7.57035509507609E-5
3	0.0	0.0	0.0	0.0	7.57035509507609E-5
4	0.0	0.0	0.0	0.0	7.57035509507609E-5
5	0.0	0.0	0.0	0.0	7.57035509507609E-5
6	0.0	0.0	0.0	0.0	1.514071019015218E-4
7	0.0	0.0	0.0	7.57035509507609E-5	1.514071019015218E-4
8	0.0	0.0	0.0	7.57035509507609E-5	1.514071019015218E-4
9	0.0	0.0	0.0	7.57035509507609E-5	1.514071019015218E-4
10	0.0	0.0	0.0	7.57035509507609E-5	1.514071019015218E-4
11	0.0	0.0	0.0	7.57035509507609E-5	1.514071019015218E-4
12	0.0	0.0	0.0	7.57035509507609E-5	3.7851775475380447E-4
13	0.0	0.0	0.0	7.57035509507609E-5	4.542213057045654E-4
14	0.0	0.0	0.0	1.514071019015218E-4	4.542213057045654E-4
15	0.0	0.0	0.0	1.514071019015218E-4	4.542213057045654E-4
16	0.0	0.0	0.0	3.028142038030436E-4	4.542213057045654E-4
17	0.0	0.0	0.0	3.7851775475380447E-4	4.542213057045654E-4
18	0.0	0.0	0.0	5.299248566553263E-4	4.542213057045654E-4
19	0.0	0.0	0.0	6.056284076060872E-4	4.542213057045654E-4
20	0.0	0.0	0.0	8.327390604583698E-4	4.542213057045654E-4
21	0.0	0.0	0.0	9.084426114091308E-4	6.056284076060872E-4
22	0.0	0.0	0.0	9.841461623598916E-4	6.81331958556848E-4
23	0.0	0.0	0.0	0.0012869603661629352	6.81331958556848E-4
24	0.0	0.0	0.0	0.0017411816718675007	6.81331958556848E-4
25	0.0	0.0	0.0	0.001968292324719783	6.81331958556848E-4
26	0.0	0.0	0.0	0.002422513630424349	6.81331958556848E-4
27	0.0	0.0	0.0	0.002801031385178153	6.81331958556848E-4
28	0.0	0.0	0.0	0.003558066894685762	6.81331958556848E-4
29	0.0	0.0	0.0	0.004845027260848698	6.81331958556848E-4
30	0.0	0.0	0.0	0.008554501257435981	6.81331958556848E-4
31	0.0	0.0	0.0	0.015367820843004462	6.81331958556848E-4
32	0.0	0.0	0.0	0.022483954632375985	6.81331958556848E-4
33	0.0	0.0	0.0	0.029297274217944466	8.327390604583698E-4
34	0.0	0.0	0.0	0.03800318257728197	9.084426114091308E-4
35	0.0	0.0	0.0	0.04973723297464991	9.084426114091308E-4
36	0.0	0.0	0.0	0.06465083251194981	9.084426114091308E-4
37	0.0	0.0	0.0	0.08387953445344307	9.084426114091308E-4
38	0.0	0.0	0.0	0.10908881692004645	9.084426114091308E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	550	0.0	32.00862	1
GTATCAA	1655	0.0	29.119625	1
AGCGACG	50	0.0025810667	21.99926	33
CGACGTT	65	4.9336127E-4	20.30701	7
ATACCGT	285	0.0	19.297598	6
TCAACGC	2615	0.0	18.507982	4
TATCAAC	2635	0.0	18.367506	2
AACGTCG	60	0.0074106553	18.334106	22
CGCACGA	60	0.007413892	18.332718	10
GGCGTTA	340	0.0	18.119097	42
TACGCTC	85	1.4313275E-4	18.117039	4
ATCAACG	2660	0.0	18.112175	3
CAACGCA	2695	0.0	17.958582	5
AACGCAG	2795	0.0	17.473475	6
AGCGTAT	140	8.376992E-8	17.285135	8
CGTCGTA	280	0.0	17.285135	10
CGGCGTT	395	0.0	16.709564	41
ACCGTCG	290	0.0	16.689095	8
TACCGTC	290	0.0	16.689095	7
ACGGACC	425	0.0	16.56415	8
>>END_MODULE