FastQCFastQC Report
Thu 2 Feb 2017
SRR4062103_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062103_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960785
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT39440.1332079161438605No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25150.013.4792211
TATTCCG701.0908081E-412.2151675
CGAACGA701.09166955E-412.21413316
GGTATCA8250.012.0856481
GTATTAT2450.010.8524191
CGATAAC803.7679746E-410.68899410
GTACTAA1909.094947E-1210.4954311
GTATTAC2600.010.2263171
GTCTTAC1502.464003E-810.1289241
CGGTCCA2002.5465852E-119.97639510
ATTGCGA1054.1013125E-59.95377210
GCGGTCG1356.43593E-79.85322959
TTACACA5150.09.7764124
GTGTACG1856.548362E-109.7525121
TACACCG2151.0913936E-119.7216325
GTATAAT2650.09.6750341
GCATATA3050.09.6515361
ACGAACG1002.755666E-49.50036415
TACGCCC800.0045185799.4997214
CTCGGTA800.00451984029.49939918
Produced by FastQC (version 0.11.5)