##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062103_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2960785
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.137242994678775	32.0	32.0	32.0	32.0	32.0
2	30.722519872263607	32.0	32.0	32.0	32.0	32.0
3	30.717575237648123	32.0	32.0	32.0	32.0	32.0
4	30.688267469606878	32.0	32.0	32.0	32.0	32.0
5	30.666472573996423	32.0	32.0	32.0	32.0	32.0
6	34.004323515554155	36.0	36.0	36.0	32.0	36.0
7	34.01442894367541	36.0	36.0	36.0	32.0	36.0
8	33.953562990895996	36.0	36.0	36.0	32.0	36.0
9	34.15611028831881	36.0	36.0	36.0	32.0	36.0
10	33.84595977080403	36.0	36.0	36.0	32.0	36.0
11	34.10854452450955	36.0	36.0	36.0	32.0	36.0
12	33.9464783832666	36.0	36.0	36.0	32.0	36.0
13	34.03542270039871	36.0	36.0	36.0	32.0	36.0
14	33.94442250957094	36.0	36.0	36.0	32.0	36.0
15	33.86793637498163	36.0	36.0	36.0	32.0	36.0
16	33.879008438640426	36.0	36.0	36.0	32.0	36.0
17	33.82152571024238	36.0	36.0	36.0	32.0	36.0
18	33.80483047570154	36.0	36.0	36.0	32.0	36.0
19	33.79464500124122	36.0	36.0	36.0	32.0	36.0
20	33.77614483996643	36.0	36.0	36.0	32.0	36.0
21	33.76491335912604	36.0	36.0	36.0	32.0	36.0
22	33.707497842632954	36.0	36.0	36.0	32.0	36.0
23	33.6910417338645	36.0	36.0	36.0	32.0	36.0
24	33.65641814586334	36.0	36.0	36.0	27.0	36.0
25	33.024357729453506	36.0	32.0	36.0	21.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	37.0
5	231.0
6	578.0
7	130.0
8	459.0
9	371.0
10	201.0
11	63.0
12	107.0
13	122.0
14	598.0
15	1217.0
16	1898.0
17	2675.0
18	3432.0
19	4178.0
20	5344.0
21	6467.0
22	8454.0
23	10878.0
24	15039.0
25	20752.0
26	29527.0
27	41159.0
28	58647.0
29	83200.0
30	119763.0
31	179042.0
32	281409.0
33	425291.0
34	818967.0
35	840547.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.056071884415886	16.98415909736476	11.580972813334986	26.378796204884363
2	16.490260330224014	18.893750223758754	37.99369625145823	26.622293194559
3	19.27528865006172	22.38205328690389	28.251313427441893	30.091344635592492
4	13.126889891126059	15.23660964003232	34.82919032267653	36.80731014616509
5	14.863736245915332	35.98346942140272	33.936506198966974	15.216288133714976
6	35.21044890720133	34.52390125339303	16.326113346094367	13.939536493311271
7	30.760731645553356	29.823235132131593	20.12383649714599	19.292196725169056
8	27.79177116628676	34.094847901070615	18.675910642766485	19.437470289876142
9	26.732242743064116	14.76604583427586	18.308249486640825	40.1934619360192
10	15.772223223594573	27.22710752901622	31.868350009282892	25.132319238106316
11	36.87074526244305	21.331240566399863	22.018119952611748	19.779894218545337
12	23.872470311969494	23.403866782276054	29.22483771174576	23.498825194008692
13	29.541178364281695	19.739691544388176	24.896083571397654	25.823046519932475
14	23.17449100520871	19.053235031081524	25.318363710442277	32.453910253267495
15	24.959774425665373	27.129588234856485	22.046928822207356	25.86370851727079
16	25.379052106836888	26.209059650515666	23.473216559034274	24.938671683613176
17	23.995516240946255	26.147416259205908	25.19040759601534	24.666659903832496
18	24.44054647199495	25.834349619953322	25.500674011880324	24.224429896171404
19	25.239877447243526	25.38608749682648	25.29007904808299	24.083956007847
20	25.346532645310464	25.070929118207307	25.13728625345857	24.44525198302366
21	25.46964116210581	24.871780830067852	24.94283716942914	24.715740838397195
22	25.38079303399094	24.678945172699876	24.995216210386673	24.94504558292251
23	24.606821061796925	24.981975742543636	25.177715967447163	25.233487228212283
24	24.434609857730894	25.126129588708523	25.262121370383404	25.17713918317718
25	24.6143856113464	24.808549144771156	25.339118577332965	25.237946666549483
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	130.0
1	130.0
2	402.5
3	675.0
4	675.0
5	675.0
6	1491.0
7	2307.0
8	2307.0
9	2307.0
10	3123.0
11	3939.0
12	3939.0
13	3939.0
14	5867.5
15	7796.0
16	7796.0
17	7796.0
18	13457.5
19	19119.0
20	19119.0
21	19119.0
22	28460.0
23	37801.0
24	37801.0
25	37801.0
26	55708.5
27	73616.0
28	73616.0
29	73616.0
30	95051.0
31	116486.0
32	116486.0
33	116486.0
34	146998.0
35	177510.0
36	177510.0
37	177510.0
38	213372.5
39	249235.0
40	249235.0
41	249235.0
42	286598.0
43	323961.0
44	323961.0
45	323961.0
46	354628.0
47	385295.0
48	385295.0
49	385295.0
50	396921.0
51	408547.0
52	408547.0
53	408547.0
54	391300.0
55	374053.0
56	374053.0
57	374053.0
58	339928.0
59	305803.0
60	305803.0
61	305803.0
62	262599.5
63	219396.0
64	219396.0
65	219396.0
66	176600.0
67	133804.0
68	133804.0
69	133804.0
70	100553.5
71	67303.0
72	67303.0
73	67303.0
74	48811.5
75	30320.0
76	30320.0
77	30320.0
78	22634.0
79	14948.0
80	14948.0
81	14948.0
82	10065.0
83	5182.0
84	5182.0
85	5182.0
86	3542.5
87	1903.0
88	1903.0
89	1903.0
90	1202.5
91	502.0
92	502.0
93	502.0
94	319.0
95	136.0
96	136.0
97	136.0
98	577.0
99	1018.0
100	1018.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001080794451471485
2	2.364237862593873E-4
3	6.754965321696779E-4
4	0.010031123502719718
5	0.03164701253214941
6	0.060017866883275886
7	0.10179732739797047
8	0.10264169806318257
9	0.11159202711443081
10	0.1260476529028619
11	0.13287016787777564
12	0.1250006332779989
13	0.12162315061715052
14	0.10943043821148783
15	0.12533838154408375
16	0.11287547052555319
17	0.11635427766622704
18	0.10544500867168674
19	0.09159732976220834
20	0.08612580785163394
21	0.08676752955719513
22	0.09723772580582515
23	0.07673640605447542
24	0.08504501340016246
25	0.08288342449721949
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2960785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.59741523459322
#Duplication Level	Percentage of deduplicated	Percentage of total
1	61.81009482219507	25.09330085186233
2	16.877898193392927	13.70398082488727
3	7.700253250107568	9.378311358184288
4	4.153266928122335	6.744476082443435
5	2.5315107545505273	5.138639663666308
6	1.5954526821645003	3.8862753014986615
7	1.0908991216636064	3.1001379234861317
8	0.7712528201362172	2.504869679193684
9	0.5661828155058714	2.068700297380465
>10	2.695496034146261	18.976861592523647
>50	0.1443904164859897	3.9936273755366147
>100	0.060217127609101126	4.321903589265012
>500	0.002668137444493046	0.7454749969702569
>1k	4.168964756753506E-4	0.34344046310180054
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	3944	0.1332079161438605	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	3.37748266084839E-5
5	0.0	0.0	0.0	0.0	3.37748266084839E-5
6	0.0	0.0	0.0	0.0	6.75496532169678E-5
7	0.0	0.0	0.0	0.0	6.75496532169678E-5
8	0.0	0.0	0.0	0.0	6.75496532169678E-5
9	0.0	0.0	0.0	1.0132447982545169E-4	1.0132447982545169E-4
10	0.0	0.0	0.0	1.0132447982545169E-4	1.0132447982545169E-4
11	0.0	0.0	0.0	1.0132447982545169E-4	1.0132447982545169E-4
12	0.0	0.0	0.0	1.0132447982545169E-4	1.0132447982545169E-4
13	0.0	0.0	0.0	1.0132447982545169E-4	1.0132447982545169E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2515	0.0	13.479221	1
TATTCCG	70	1.0908081E-4	12.215167	5
CGAACGA	70	1.09166955E-4	12.214133	16
GGTATCA	825	0.0	12.085648	1
GTATTAT	245	0.0	10.852419	1
CGATAAC	80	3.7679746E-4	10.688994	10
GTACTAA	190	9.094947E-12	10.495431	1
GTATTAC	260	0.0	10.226317	1
GTCTTAC	150	2.464003E-8	10.128924	1
CGGTCCA	200	2.5465852E-11	9.976395	10
ATTGCGA	105	4.1013125E-5	9.953772	10
GCGGTCG	135	6.43593E-7	9.8532295	9
TTACACA	515	0.0	9.776412	4
GTGTACG	185	6.548362E-10	9.752512	1
TACACCG	215	1.0913936E-11	9.721632	5
GTATAAT	265	0.0	9.675034	1
GCATATA	305	0.0	9.651536	1
ACGAACG	100	2.755666E-4	9.500364	15
TACGCCC	80	0.004518579	9.499721	4
CTCGGTA	80	0.0045198402	9.499399	18
>>END_MODULE