FastQCFastQC Report
Thu 2 Feb 2017
SRR4062103_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062103_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2960785
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA25750.027.7774581
GGTATCA11500.022.965181
CAACGCA37500.018.6551555
TCAACGC37500.018.596494
ATCAACG37800.018.5652963
TATCAAC38900.018.2677572
AACGCAG39900.017.4779056
CGGATCG2650.016.60330226
TTAGTCG1105.622152E-516.00116739
ATCGATT2650.015.77313629
GGATCGA2800.015.71383927
GTATTAT3450.015.3101191
TAGACGT1301.370865E-515.2300794
GTACCGT3750.014.6660026
TACCGTA1201.18239164E-414.6660027
ACGCAGA48900.014.4410647
GTACTAA1851.2617056E-714.2756511
GGCGTTA3550.014.25504242
TAATACT4500.014.1771354
GTATAGG4750.013.8999771
Produced by FastQC (version 0.11.5)