##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062103_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2960785
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.061055091808424	32.0	32.0	32.0	32.0	32.0
2	30.948049588200426	32.0	32.0	32.0	32.0	32.0
3	31.29150039600984	32.0	32.0	32.0	32.0	32.0
4	31.461601568502946	32.0	32.0	32.0	32.0	32.0
5	31.37002045065751	32.0	32.0	32.0	32.0	32.0
6	34.97622725054335	36.0	36.0	36.0	36.0	36.0
7	34.99556637851111	36.0	36.0	36.0	36.0	36.0
8	34.91547984740534	36.0	36.0	36.0	36.0	36.0
9	35.085394582855564	36.0	36.0	36.0	36.0	36.0
10	34.865835243018324	36.0	36.0	36.0	32.0	36.0
11	35.09563207054886	36.0	36.0	36.0	36.0	36.0
12	34.981227613622735	36.0	36.0	36.0	36.0	36.0
13	35.04608777739687	36.0	36.0	36.0	36.0	36.0
14	34.99184202838099	36.0	36.0	36.0	32.0	36.0
15	34.9395542060636	36.0	36.0	36.0	32.0	36.0
16	34.96721274932155	36.0	36.0	36.0	36.0	36.0
17	34.94784491275118	36.0	36.0	36.0	32.0	36.0
18	34.94138446391751	36.0	36.0	36.0	32.0	36.0
19	34.93940323258865	36.0	36.0	36.0	32.0	36.0
20	34.94372742363934	36.0	36.0	36.0	32.0	36.0
21	34.932564843445235	36.0	36.0	36.0	32.0	36.0
22	34.91274746393271	36.0	36.0	36.0	32.0	36.0
23	34.87464270455302	36.0	36.0	36.0	32.0	36.0
24	34.86274079340445	36.0	36.0	36.0	32.0	36.0
25	34.8496506838558	36.0	36.0	36.0	32.0	36.0
26	34.78703316856847	36.0	36.0	36.0	32.0	36.0
27	34.770280516822396	36.0	36.0	36.0	32.0	36.0
28	34.75323773931576	36.0	36.0	36.0	32.0	36.0
29	34.72574232847032	36.0	36.0	36.0	32.0	36.0
30	34.7099461798138	36.0	36.0	36.0	32.0	36.0
31	34.710829053781346	36.0	36.0	36.0	32.0	36.0
32	34.676520247164184	36.0	36.0	36.0	32.0	36.0
33	34.65600609297872	36.0	36.0	36.0	32.0	36.0
34	34.64086010973441	36.0	36.0	36.0	32.0	36.0
35	34.64007619600883	36.0	36.0	36.0	32.0	36.0
36	34.601850860498146	36.0	36.0	36.0	32.0	36.0
37	34.59349260415734	36.0	36.0	36.0	32.0	36.0
38	34.58346283164769	36.0	36.0	36.0	32.0	36.0
39	34.55874810227693	36.0	36.0	36.0	32.0	36.0
40	34.552891885091285	36.0	36.0	36.0	32.0	36.0
41	34.51861347581807	36.0	36.0	36.0	32.0	36.0
42	34.4984748977045	36.0	36.0	36.0	32.0	36.0
43	34.48601705290996	36.0	36.0	36.0	32.0	36.0
44	34.44203175846946	36.0	36.0	36.0	32.0	36.0
45	34.43047705253843	36.0	36.0	36.0	32.0	36.0
46	34.41268278513975	36.0	36.0	36.0	32.0	36.0
47	34.38665894348965	36.0	36.0	36.0	32.0	36.0
48	34.359611386845046	36.0	36.0	36.0	32.0	36.0
49	34.34084541768484	36.0	36.0	36.0	32.0	36.0
50	33.84000493112468	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	21.0
21	55.0
22	127.0
23	452.0
24	1266.0
25	3126.0
26	6923.0
27	14081.0
28	25633.0
29	43034.0
30	68324.0
31	108131.0
32	173657.0
33	307484.0
34	664613.0
35	1543854.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.772145449827974	17.52571642472777	12.060469409211425	26.641668716232836
2	16.118406942210058	19.605217871219057	37.221393393592194	27.054981792978687
3	18.840299231858083	23.046047647563263	28.3611317419289	29.752521378649753
4	12.985547071316244	15.825031478149032	35.078783187155835	36.11063826337889
5	14.926075348260682	36.268962454214	33.29552804408291	15.509434153442413
6	35.42145284590355	34.84788815927684	16.026137689299652	13.70452130551996
7	30.876102114810767	29.89474075287466	19.998311258669577	19.230845873645
8	27.986834572588016	34.199308629299324	18.461894396249644	19.35196240186302
9	26.9353904454393	14.530639678328553	18.038797143325166	40.49517273290698
10	15.747134201122678	27.286154324198353	31.841237781935845	25.125473692743128
11	37.33570657781636	21.16975734475823	21.803440641586604	19.691095435838808
12	24.101614943334283	23.29642307698803	29.05628068231905	23.54568129735864
13	29.7619381346501	19.611994791921735	24.782954520507232	25.843112552920932
14	23.362216439221356	18.80065590713274	25.144007416951926	32.693120236693986
15	25.200951774613827	27.154893043567835	21.79449031253536	25.84966486928298
16	25.635173036185204	26.11795317971553	23.386263293848085	24.86061049025118
17	24.275791724154235	25.981859540628584	25.080713391887627	24.661635343329554
18	24.746468664655033	25.732256859514628	25.325877184983135	24.195397290847204
19	25.48874707214472	25.216386870373903	25.16548820667492	24.129377850806456
20	25.630281760307	24.88405922900696	25.001359439525885	24.484299571160157
21	25.754825156166355	24.783562467386183	24.781468428136456	24.680143948311006
22	25.61084307033439	24.611648599949	24.90393594941882	24.873572380297794
23	24.848486076660777	24.774856929786164	25.085788088560328	25.29086890499273
24	24.75759635013125	25.011111996768403	25.05022352338788	25.181068129712465
25	24.968293817269036	24.582280541708787	25.30459044731133	25.144835193710847
26	24.80230669490704	25.15332989053872	25.155964337691543	24.888399076862697
27	25.09993153824148	25.25340476503296	24.735162425268772	24.911501271456782
28	25.030288107708465	24.96652011512014	25.0532891685944	24.949902608577
29	24.96609840328968	25.07147621821989	25.057527167717915	24.904898210772515
30	25.065523893929647	25.070860376012583	25.01283457716148	24.850781152896293
31	24.96650370257787	24.88378887284579	25.007878004914254	25.14182941966208
32	25.231941588108757	25.05425114505802	24.69002120058755	25.023786066245673
33	24.775363248873607	25.091327285377503	24.92035882436385	25.21295064138504
34	25.099357767333778	24.969391356625735	25.17315626197959	24.758094614060894
35	25.165539204378305	24.944414966466596	25.116464281866357	24.773581547288735
36	24.976442002743873	25.07395015769504	24.95735918059375	24.992248658967338
37	25.24924495278548	24.880756242860745	25.014574049343036	24.855424755010738
38	25.009178309130853	24.948957793287928	25.032246515704447	25.009617381876765
39	25.212495685238213	25.139845566991788	24.81415282396515	24.833505923804854
40	25.407772751698115	24.8650777379872	24.88767314277763	24.83947636753706
41	24.777421712562603	25.184716335962843	25.072143736261953	24.9657182152126
42	25.30980433748027	25.00885782523418	24.966806376553325	24.71453146073222
43	25.04823255386644	24.696384855248297	25.12743797320594	25.127944617679326
44	24.859229165899666	25.002170122030098	25.11190905550547	25.026691656564765
45	25.00661156522347	25.18798630468727	24.885458643350724	24.919943486738536
46	24.944430690268607	24.98175273084189	24.97320750345271	25.100609075436797
47	24.989251030735804	25.130499073038614	25.036772789588042	24.843477106637543
48	25.16514842924068	25.369054977420653	24.671881702452485	24.793914890886185
49	25.027063012149565	25.162741164792042	24.823967983063365	24.986227839995028
50	25.058296394270062	25.230686195170655	24.745941206431382	24.965076204127897
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	23.0
1	28.0
2	33.0
3	127.0
4	221.0
5	235.0
6	249.0
7	218.5
8	188.0
9	272.0
10	356.0
11	550.0
12	744.0
13	1394.5
14	2045.0
15	2716.0
16	3387.0
17	3487.5
18	3588.0
19	3913.5
20	4239.0
21	5305.0
22	6371.0
23	8632.0
24	10893.0
25	13961.0
26	17029.0
27	21322.0
28	25615.0
29	30067.5
30	34520.0
31	40558.5
32	46597.0
33	54349.0
34	62101.0
35	71989.5
36	81878.0
37	91625.0
38	101372.0
39	115442.0
40	129512.0
41	140256.5
42	151001.0
43	161109.5
44	171218.0
45	185303.5
46	199389.0
47	207683.0
48	215977.0
49	222857.5
50	229738.0
51	232391.0
52	235044.0
53	234570.5
54	234097.0
55	228248.5
56	222400.0
57	212127.5
58	201855.0
59	185532.5
60	169210.0
61	149162.0
62	129114.0
63	110950.0
64	92786.0
65	77606.0
66	62426.0
67	52358.5
68	42291.0
69	35468.5
70	28646.0
71	22672.5
72	16699.0
73	13997.5
74	11296.0
75	9150.0
76	7004.0
77	5637.5
78	4271.0
79	3450.0
80	2629.0
81	2020.0
82	1411.0
83	1035.0
84	659.0
85	493.5
86	328.0
87	237.0
88	146.0
89	108.5
90	71.0
91	51.0
92	31.0
93	32.5
94	34.0
95	26.5
96	19.0
97	21.5
98	24.0
99	17.0
100	10.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027323834726263475
2	0.003850330233367164
3	1.3509930643393561E-4
4	3.3774826608483904E-5
5	0.0
6	1.0132447982545169E-4
7	0.0
8	0.0
9	0.0
10	1.6887413304241948E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.741720523442262E-4
17	0.0
18	2.364237862593873E-4
19	0.0
20	2.0264895965090338E-4
21	0.0
22	0.0
23	3.3774826608483904E-5
24	7.092713587781618E-4
25	2.0264895965090338E-4
26	4.0529791930180676E-4
27	2.7019861286787123E-4
28	0.0017562909836411626
29	3.3774826608483897E-4
30	0.0011145692780799685
31	3.3774826608483897E-4
32	6.079468789527101E-4
33	1.6887413304241948E-4
34	6.754965321696779E-4
35	2.0264895965090338E-4
36	2.364237862593873E-4
37	0.0014523175441648077
38	0.0
39	6.41721705561194E-4
40	2.0264895965090338E-4
41	9.79469971646033E-4
42	0.004255628152668971
43	0.004356952632494423
44	0.004120528846235035
45	0.00216158890294297
46	0.002296688209376905
47	0.0012158937579054203
48	0.0038841050599756483
49	0.0034450323140653577
50	0.0017900658102496466
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2960785.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.043652420409714
#Duplication Level	Percentage of deduplicated	Percentage of total
1	63.27440861904774	27.235616517052673
2	16.511159960145694	14.214012607645943
3	7.582038886163134	9.79075939362109
4	3.910997144920291	6.733744068926552
5	2.363332295790862	5.086322694697539
6	1.5217113435808867	3.9300008494374206
7	1.0261648189737034	3.0918917255769713
8	0.7018157116804731	2.416696924540541
9	0.5330270626013629	2.064908845195653
>10	2.4056784330616257	17.846314299901067
>50	0.11860605143843642	3.4664515911114004
>100	0.04940328267507702	3.6208398305175478
>500	0.001498638499671444	0.42672995150522236
>1k	1.577514209841066E-4	0.07571070027037156
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	6.75496532169678E-5
2	0.0	0.0	0.0	0.0	6.75496532169678E-5
3	0.0	0.0	0.0	0.0	6.75496532169678E-5
4	0.0	0.0	0.0	3.37748266084839E-5	6.75496532169678E-5
5	0.0	0.0	0.0	3.37748266084839E-5	6.75496532169678E-5
6	0.0	0.0	0.0	3.37748266084839E-5	1.0132447982545169E-4
7	0.0	0.0	0.0	3.37748266084839E-5	1.0132447982545169E-4
8	0.0	0.0	0.0	3.37748266084839E-5	1.350993064339356E-4
9	0.0	0.0	0.0	6.75496532169678E-5	2.0264895965090338E-4
10	0.0	0.0	0.0	6.75496532169678E-5	2.0264895965090338E-4
11	0.0	0.0	0.0	6.75496532169678E-5	2.0264895965090338E-4
12	0.0	0.0	0.0	6.75496532169678E-5	2.0264895965090338E-4
13	0.0	0.0	0.0	6.75496532169678E-5	2.0264895965090338E-4
14	0.0	0.0	0.0	6.75496532169678E-5	2.0264895965090338E-4
15	0.0	0.0	0.0	6.75496532169678E-5	3.0397343947635507E-4
16	0.0	0.0	0.0	6.75496532169678E-5	3.0397343947635507E-4
17	0.0	0.0	0.0	6.75496532169678E-5	3.0397343947635507E-4
18	0.0	0.0	0.0	6.75496532169678E-5	3.0397343947635507E-4
19	0.0	0.0	0.0	6.75496532169678E-5	3.0397343947635507E-4
20	0.0	0.0	0.0	1.0132447982545169E-4	3.0397343947635507E-4
21	0.0	0.0	0.0	1.0132447982545169E-4	3.7152309269332287E-4
22	0.0	0.0	0.0	1.0132447982545169E-4	3.7152309269332287E-4
23	0.0	0.0	0.0	1.0132447982545169E-4	4.7284757251877456E-4
24	0.0	0.0	0.0	1.0132447982545169E-4	4.7284757251877456E-4
25	0.0	0.0	0.0	1.350993064339356E-4	5.403972257357424E-4
26	0.0	0.0	0.0	1.350993064339356E-4	5.741720523442262E-4
27	0.0	0.0	0.0	1.350993064339356E-4	5.741720523442262E-4
28	0.0	0.0	0.0	1.350993064339356E-4	5.741720523442262E-4
29	0.0	0.0	0.0	1.350993064339356E-4	5.741720523442262E-4
30	0.0	0.0	0.0	1.6887413304241948E-4	5.741720523442262E-4
31	0.0	0.0	0.0	3.3774826608483897E-4	6.41721705561194E-4
32	0.0	0.0	0.0	5.741720523442262E-4	6.754965321696779E-4
33	0.0	0.0	0.0	9.456951450375491E-4	7.092713587781618E-4
34	0.0	0.0	0.0	0.001317218237730872	8.781454918205813E-4
35	0.0	0.0	0.0	0.0017225161570326789	8.781454918205813E-4
36	0.0	0.0	0.0	0.002364237862593873	8.781454918205813E-4
37	0.0	0.0	0.0	0.0034450323140653577	8.781454918205813E-4
38	0.0	0.0	0.0	0.005066223991272585	9.456951450375491E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2575	0.0	27.777458	1
GGTATCA	1150	0.0	22.96518	1
CAACGCA	3750	0.0	18.655155	5
TCAACGC	3750	0.0	18.59649	4
ATCAACG	3780	0.0	18.565296	3
TATCAAC	3890	0.0	18.267757	2
AACGCAG	3990	0.0	17.477905	6
CGGATCG	265	0.0	16.603302	26
TTAGTCG	110	5.622152E-5	16.001167	39
ATCGATT	265	0.0	15.773136	29
GGATCGA	280	0.0	15.713839	27
GTATTAT	345	0.0	15.310119	1
TAGACGT	130	1.370865E-5	15.230079	4
GTACCGT	375	0.0	14.666002	6
TACCGTA	120	1.18239164E-4	14.666002	7
ACGCAGA	4890	0.0	14.441064	7
GTACTAA	185	1.2617056E-7	14.275651	1
GGCGTTA	355	0.0	14.255042	42
TAATACT	450	0.0	14.177135	4
GTATAGG	475	0.0	13.899977	1
>>END_MODULE