FastQCFastQC Report
Thu 2 Feb 2017
SRR4062102_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062102_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1546745
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26300.17003449178759267No Hit
TATCAACGCAGAGTACTTTTTTTTT18090.11695528351473579No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGATCG704.4336048E-714.9292575
TATGCCG400.005277472514.2506555
AAGACGG2500.013.680635
GTATAGG1351.8189894E-1213.3666591
GAGCGTT500.001498654813.3010427
TACCGTA500.001498654813.3010427
GCGTTAT1600.013.0588741
CGTTATT1650.012.66522
CAAGACG2850.012.3331024
CGCAAGA2800.012.2128722
TAGGACC6200.012.1046114
GATCGAA550.003065143512.0926411
CGCATCG2100.011.76206513
GTATCAA16850.011.7237481
GGATCGT658.015925E-411.6936036
CGTCAAT2050.011.58327419
ATTACCG2550.011.54880415
TTACCGC2550.011.54880416
TTTTCGG1751.8189894E-1211.39978716
ATCGCCA2250.011.39978616
Produced by FastQC (version 0.11.5)