##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062102_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1546745
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205772121455055	32.0	32.0	32.0	32.0	32.0
2	30.686124086387867	32.0	32.0	32.0	32.0	32.0
3	30.72129730498563	32.0	32.0	32.0	32.0	32.0
4	30.7289805365461	32.0	32.0	32.0	32.0	32.0
5	30.627586964884323	32.0	32.0	32.0	32.0	32.0
6	34.29006979172391	36.0	36.0	36.0	32.0	36.0
7	34.22745572153134	36.0	36.0	36.0	32.0	36.0
8	34.17162363544088	36.0	36.0	36.0	32.0	36.0
9	34.320567385057004	36.0	36.0	36.0	32.0	36.0
10	33.96205580105318	36.0	36.0	36.0	32.0	36.0
11	34.322458453074034	36.0	36.0	36.0	32.0	36.0
12	34.100594474202275	36.0	36.0	36.0	32.0	36.0
13	34.20966804482963	36.0	36.0	36.0	32.0	36.0
14	34.10120478811957	36.0	36.0	36.0	32.0	36.0
15	34.048743651991764	36.0	36.0	36.0	32.0	36.0
16	34.059072439219136	36.0	36.0	36.0	32.0	36.0
17	33.9822834403861	36.0	36.0	36.0	32.0	36.0
18	33.995471134543834	36.0	36.0	36.0	32.0	36.0
19	33.99416710576081	36.0	36.0	36.0	32.0	36.0
20	33.97820843125402	36.0	36.0	36.0	32.0	36.0
21	33.95734203116868	36.0	36.0	36.0	32.0	36.0
22	33.92442904292563	36.0	36.0	36.0	32.0	36.0
23	33.893016625235575	36.0	36.0	36.0	32.0	36.0
24	33.85464443072387	36.0	36.0	36.0	32.0	36.0
25	33.49347500719252	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	2.0
4	23.0
5	130.0
6	342.0
7	72.0
8	255.0
9	209.0
10	97.0
11	35.0
12	78.0
13	77.0
14	196.0
15	295.0
16	440.0
17	671.0
18	941.0
19	1278.0
20	2088.0
21	3120.0
22	4728.0
23	7139.0
24	10302.0
25	14409.0
26	19852.0
27	25628.0
28	33740.0
29	44134.0
30	58212.0
31	78046.0
32	108811.0
33	151500.0
34	320954.0
35	658941.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27438837134576	17.28025105852278	11.342437696018246	26.10292287411321
2	16.717278458384435	19.1230464570345	37.90957891463406	26.250096169947007
3	19.5206298675018	22.525922295825147	27.979399225983975	29.974048610689074
4	13.065883866712792	15.260460951063148	34.833584962999126	36.840070219224934
5	15.055377521091964	36.157171914461536	33.32307164961955	15.464378914826948
6	35.184197996025176	34.4921616222259	16.38160142017224	13.94203896157668
7	31.07808645091476	29.74762929193299	19.9580223518356	19.21626190531665
8	27.933172778515253	33.92524168979955	18.792680408968202	19.348905122716996
9	26.955055488848828	14.584586107236367	18.620238784909105	39.8401196190057
10	16.71485356190279	26.70022269984722	30.923427506020662	25.661496232229332
11	37.108498945983605	21.125971792760176	22.01223902087833	19.75329024037789
12	24.58021859642785	23.496882511703124	28.74809101991166	23.174807871957366
13	29.80921110552946	19.518962365285123	24.84398709430171	25.82783943488371
14	23.23591075001343	19.827447264225505	24.807190538792	32.129451446969064
15	24.993493739099584	27.813909285243543	21.726962333604803	25.46563464205207
16	25.513833518781386	25.737665762405115	24.095948817631093	24.652551901182402
17	23.941211198458813	26.304777884804608	25.215434649763335	24.538576266973248
18	24.541603425231635	25.338040977213666	26.217908679907186	23.902446917647516
19	25.63375924785015	25.01805563465232	25.032810776948843	24.315374340548683
20	25.681812887143657	24.780235709496974	24.95133605816894	24.586615345190424
21	26.11022996338006	24.45214073600965	24.4606178732998	24.97701142731049
22	25.797977679923576	24.805909565118345	24.769149522766636	24.626963232191443
23	24.46308553296093	24.78428626983321	25.397893559873463	25.354734637332406
24	24.8472792408484	24.928816595310696	25.40322163147187	24.820682532369034
25	24.809542390528534	24.786829192219013	25.42486946381969	24.978758953432767
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	238.0
1	238.0
2	370.5
3	503.0
4	503.0
5	503.0
6	1140.5
7	1778.0
8	1778.0
9	1778.0
10	2097.5
11	2417.0
12	2417.0
13	2417.0
14	3196.0
15	3975.0
16	3975.0
17	3975.0
18	6883.5
19	9792.0
20	9792.0
21	9792.0
22	15413.0
23	21034.0
24	21034.0
25	21034.0
26	31607.0
27	42180.0
28	42180.0
29	42180.0
30	55089.0
31	67998.0
32	67998.0
33	67998.0
34	81405.0
35	94812.0
36	94812.0
37	94812.0
38	109674.0
39	124536.0
40	124536.0
41	124536.0
42	142164.0
43	159792.0
44	159792.0
45	159792.0
46	178183.5
47	196575.0
48	196575.0
49	196575.0
50	202667.5
51	208760.0
52	208760.0
53	208760.0
54	197446.0
55	186132.0
56	186132.0
57	186132.0
58	171500.0
59	156868.0
60	156868.0
61	156868.0
62	137351.0
63	117834.0
64	117834.0
65	117834.0
66	96197.5
67	74561.0
68	74561.0
69	74561.0
70	56750.0
71	38939.0
72	38939.0
73	38939.0
74	29199.0
75	19459.0
76	19459.0
77	19459.0
78	15205.0
79	10951.0
80	10951.0
81	10951.0
82	7618.0
83	4285.0
84	4285.0
85	4285.0
86	3109.5
87	1934.0
88	1934.0
89	1934.0
90	1293.0
91	652.0
92	652.0
93	652.0
94	419.0
95	186.0
96	186.0
97	186.0
98	370.0
99	554.0
100	554.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.05126572253345E-4
2	2.586075920723843E-4
3	4.525632861266725E-4
4	0.010344303682895371
5	0.03491202492977188
6	0.06575098028440371
7	0.10583515705562326
8	0.10900310005850997
9	0.11895949235329675
10	0.13350616940736837
11	0.14074718198539515
12	0.13395873269349504
13	0.12936844793421023
14	0.11805436578104342
15	0.13441129597962173
16	0.11883018855726056
17	0.12678237201348638
18	0.11249430255148715
19	0.09833553688552413
20	0.09393920782029358
21	0.09128848000155165
22	0.10273186595075463
23	0.0836595560354163
24	0.09316338504407644
25	0.08973683444911734
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1546745.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.491732132056256
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.26948847769597	41.5605653597556
2	14.071641688113592	15.335762590539236
3	4.266074037523546	6.973972911247582
4	1.7716624368874372	3.8616381975718506
5	0.9499322593824867	2.588172711093472
6	0.5797652543866558	1.8955447764906659
7	0.35379398644695853	1.3495192997580003
8	0.26694828402033133	1.1637179508758375
9	0.20298920312817065	0.9955109954303848
>10	1.1072595852654858	10.96271030493852
>50	0.08895745639178339	3.349236890559762
>100	0.06420745046448939	6.841151590426661
>500	0.00572843039463685	2.0438158394045036
>1k	0.00155144989854748	1.0786805819079324
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2630	0.17003449178759267	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1809	0.11695528351473579	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.2930379603619214E-4
2	0.0	0.0	0.0	0.0	1.2930379603619214E-4
3	0.0	0.0	0.0	0.0	1.2930379603619214E-4
4	0.0	0.0	0.0	0.0	1.2930379603619214E-4
5	0.0	0.0	0.0	0.0	1.2930379603619214E-4
6	0.0	0.0	0.0	0.0	1.9395569405428819E-4
7	0.0	0.0	0.0	0.0	1.9395569405428819E-4
8	0.0	0.0	0.0	0.0	1.9395569405428819E-4
9	0.0	0.0	0.0	0.0	1.9395569405428819E-4
10	0.0	0.0	0.0	0.0	2.586075920723843E-4
11	0.0	0.0	0.0	0.0	2.586075920723843E-4
12	0.0	0.0	0.0	0.0	5.172151841447686E-4
13	0.0	0.0	0.0	6.465189801809607E-5	6.465189801809607E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGGATCG	70	4.4336048E-7	14.929257	5
TATGCCG	40	0.0052774725	14.250655	5
AAGACGG	250	0.0	13.68063	5
GTATAGG	135	1.8189894E-12	13.366659	1
GAGCGTT	50	0.0014986548	13.301042	7
TACCGTA	50	0.0014986548	13.301042	7
GCGTTAT	160	0.0	13.058874	1
CGTTATT	165	0.0	12.6652	2
CAAGACG	285	0.0	12.333102	4
CGCAAGA	280	0.0	12.212872	2
TAGGACC	620	0.0	12.104611	4
GATCGAA	55	0.0030651435	12.09264	11
CGCATCG	210	0.0	11.762065	13
GTATCAA	1685	0.0	11.723748	1
GGATCGT	65	8.015925E-4	11.693603	6
CGTCAAT	205	0.0	11.583274	19
ATTACCG	255	0.0	11.548804	15
TTACCGC	255	0.0	11.548804	16
TTTTCGG	175	1.8189894E-12	11.399787	16
ATCGCCA	225	0.0	11.399786	16
>>END_MODULE