FastQCFastQC Report
Thu 2 Feb 2017
SRR4062101_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062101_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207140
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT26350.11938526781264441No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20400.012.7535571
ACGCTAC604.1101722E-412.6604661
CGCGTAA1900.012.50238910
CAAGACG4500.012.4539794
CGGACCA4200.012.4420069
CGCAAGA4150.012.358762
GGTATCA8500.012.2880991
TCGCGTA1950.012.1809859
AAGACGG4700.012.1277755
CGAGCCG3300.011.80421515
CGAACGA1251.8328137E-811.40010916
AGACGGA5200.011.3278026
GCTCGTA1604.5474735E-1111.2826389
ACGAACG1354.7239155E-911.26038915
GCGTAAC2200.011.22941911
GACGGAC5300.011.1140717
CGTAGAC600.005885500511.0811773
ACGGTAT2750.011.0559059
CGGTCGG1553.1286618E-1011.03436810
CGGTCCA3100.011.03436810
Produced by FastQC (version 0.11.5)