##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062101_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2207140
Sequences flagged as poor quality	0
Sequence length	25
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.227198093460316	32.0	32.0	32.0	32.0	32.0
2	30.786519205850105	32.0	32.0	32.0	32.0	32.0
3	30.81752947253006	32.0	32.0	32.0	32.0	32.0
4	30.82885997263427	32.0	32.0	32.0	32.0	32.0
5	30.75548220774396	32.0	32.0	32.0	32.0	32.0
6	34.382939913190825	36.0	36.0	36.0	32.0	36.0
7	34.324344173908315	36.0	36.0	36.0	32.0	36.0
8	34.279068840218564	36.0	36.0	36.0	32.0	36.0
9	34.41315820473554	36.0	36.0	36.0	32.0	36.0
10	34.12228358871662	36.0	36.0	36.0	32.0	36.0
11	34.391341283289684	36.0	36.0	36.0	32.0	36.0
12	34.23019880931885	36.0	36.0	36.0	32.0	36.0
13	34.31099839611443	36.0	36.0	36.0	32.0	36.0
14	34.21642578178095	36.0	36.0	36.0	32.0	36.0
15	34.17222332973894	36.0	36.0	36.0	32.0	36.0
16	34.17997952100909	36.0	36.0	36.0	32.0	36.0
17	34.11577063530179	36.0	36.0	36.0	32.0	36.0
18	34.11531484183151	36.0	36.0	36.0	32.0	36.0
19	34.12352818579701	36.0	36.0	36.0	32.0	36.0
20	34.11357639297915	36.0	36.0	36.0	32.0	36.0
21	34.09501255017806	36.0	36.0	36.0	32.0	36.0
22	34.06690966590248	36.0	36.0	36.0	32.0	36.0
23	34.03675661716067	36.0	36.0	36.0	32.0	36.0
24	34.00808467065977	36.0	36.0	36.0	32.0	36.0
25	33.65490816169341	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	27.0
5	176.0
6	465.0
7	94.0
8	388.0
9	304.0
10	185.0
11	52.0
12	84.0
13	95.0
14	259.0
15	369.0
16	663.0
17	910.0
18	1355.0
19	1895.0
20	2734.0
21	4011.0
22	6105.0
23	9089.0
24	12868.0
25	18201.0
26	25749.0
27	33356.0
28	44170.0
29	58797.0
30	77533.0
31	105057.0
32	147560.0
33	208935.0
34	455952.0
35	989702.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.27747849465776	17.21582727945754	11.474152359221902	26.0325418666628
2	16.834833164034148	19.102656924940806	37.37813403602312	26.684375875001926
3	19.44331300979689	22.304767868121893	28.008648339086662	30.243270782994557
4	12.86935709628263	15.300676194623339	35.13298464050851	36.69698206858552
5	15.038411160311641	36.08727378206029	33.518009055516025	15.356306002112047
6	35.3425764227775	34.734938950636426	16.21983573885779	13.702648887728289
7	31.01145111742169	29.99165896742643	19.93248253893956	19.06440737621232
8	28.12905947240466	33.49209876991183	18.803702964548783	19.57513879313473
9	26.566118291085868	14.839708216470681	18.591878198838653	40.002295293604796
10	15.879092312201196	27.239571471476648	31.292300042555237	25.58903617376692
11	37.21412014088744	21.502439396214843	21.626439579515512	19.657000883382207
12	24.12279070105063	23.76796905552385	28.73529062165696	23.373949621768563
13	29.243267225939213	19.665271207262922	24.993104361854055	26.098357204943813
14	23.113853941987767	19.64573027872091	25.0979458253101	32.14246995398122
15	24.926731185566542	27.42762492899957	21.935639584577164	25.710004300856724
16	25.544550682746664	26.30278006856952	23.488644477286684	24.664024771397127
17	23.97792957533077	26.43773946447057	24.988273442454584	24.596057517744075
18	24.609816711715002	25.743501632217807	25.669068690129905	23.97761296593729
19	25.207281355698523	25.607059312264003	25.215535421279345	23.970123910758133
20	25.430056324997956	24.89206642903141	24.972925906778045	24.704951339192586
21	26.015505816495153	24.77738051832382	24.738380160971708	24.468733504209318
22	25.47134298167239	24.745676615854904	25.147695782517793	24.635284619954916
23	24.34833395229421	24.855020316880765	25.544628893275274	25.25201683754975
24	24.40679318903392	25.284262644942995	25.37827237061872	24.930671795404365
25	24.527992149301138	24.97521715036682	25.25365575374781	25.243134946584227
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	96.0
1	96.0
2	344.0
3	592.0
4	592.0
5	592.0
6	1247.5
7	1903.0
8	1903.0
9	1903.0
10	2595.0
11	3287.0
12	3287.0
13	3287.0
14	4791.5
15	6296.0
16	6296.0
17	6296.0
18	10633.0
19	14970.0
20	14970.0
21	14970.0
22	23067.5
23	31165.0
24	31165.0
25	31165.0
26	44549.0
27	57933.0
28	57933.0
29	57933.0
30	73283.0
31	88633.0
32	88633.0
33	88633.0
34	111292.5
35	133952.0
36	133952.0
37	133952.0
38	158349.0
39	182746.0
40	182746.0
41	182746.0
42	210430.0
43	238114.0
44	238114.0
45	238114.0
46	262842.0
47	287570.0
48	287570.0
49	287570.0
50	296154.0
51	304738.0
52	304738.0
53	304738.0
54	286466.0
55	268194.0
56	268194.0
57	268194.0
58	244061.0
59	219928.0
60	219928.0
61	219928.0
62	191616.5
63	163305.0
64	163305.0
65	163305.0
66	132651.0
67	101997.0
68	101997.0
69	101997.0
70	76725.0
71	51453.0
72	51453.0
73	51453.0
74	38574.0
75	25695.0
76	25695.0
77	25695.0
78	20111.0
79	14527.0
80	14527.0
81	14527.0
82	10076.0
83	5625.0
84	5625.0
85	5625.0
86	4055.5
87	2486.0
88	2486.0
89	2486.0
90	1656.5
91	827.0
92	827.0
93	827.0
94	520.0
95	213.0
96	213.0
97	213.0
98	554.0
99	895.0
100	895.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	6.343050282265737E-4
2	9.061500403236769E-5
3	4.983825221780223E-4
4	0.010466032965738467
5	0.03398062651213788
6	0.06270558279039844
7	0.107695932292469
8	0.10964415487916489
9	0.11911342280054732
10	0.1334759009396776
11	0.14095163877234793
12	0.13293221091548338
13	0.12903576574209158
14	0.11802604275215892
15	0.13266036590338628
16	0.12060857036708139
17	0.12423317052837608
18	0.11195483748199027
19	0.09813604936705418
20	0.09423960419366238
21	0.09206484409688556
22	0.1028480295767373
23	0.08295803619163261
24	0.0928350716311607
25	0.0899806990041411
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	2207140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.349714909644106
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.06930544910772	35.27969553279346
2	16.064942530489876	16.177305528999636
3	6.1648162492815874	9.311902218650081
4	2.8462002091614282	5.732214764281893
5	1.504567080748846	3.787726178906994
6	0.896128057696045	2.7071875336517377
7	0.5496223921468352	1.9371331526784836
8	0.3773268394165652	1.5198639033904895
9	0.27059346973201265	1.2261873651676423
>10	1.1117206348491298	9.62522599094358
>50	0.07528566964333014	2.600077647042989
>100	0.060560745354131286	6.153216314881354
>500	0.006675452076444433	2.288117273941812
>1k	0.002255220296096003	1.654146594669876
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	2635	0.11938526781264441	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	4.530750201618384E-5
2	0.0	0.0	0.0	0.0	4.530750201618384E-5
3	0.0	0.0	0.0	0.0	4.530750201618384E-5
4	0.0	0.0	0.0	4.530750201618384E-5	4.530750201618384E-5
5	0.0	0.0	0.0	4.530750201618384E-5	9.061500403236767E-5
6	0.0	0.0	0.0	4.530750201618384E-5	2.265375100809192E-4
7	0.0	0.0	0.0	4.530750201618384E-5	2.265375100809192E-4
8	0.0	0.0	0.0	4.530750201618384E-5	2.265375100809192E-4
9	0.0	0.0	0.0	1.3592250604855152E-4	3.171525141132869E-4
10	0.0	0.0	0.0	1.3592250604855152E-4	3.624600161294707E-4
11	0.0	0.0	0.0	1.8123000806473535E-4	4.0776751814565454E-4
12	4.530750201618384E-5	0.0	0.0	2.265375100809192E-4	6.796125302427576E-4
13	4.530750201618384E-5	0.0	0.0	2.265375100809192E-4	7.702275342751253E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2040	0.0	12.753557	1
ACGCTAC	60	4.1101722E-4	12.660466	1
CGCGTAA	190	0.0	12.502389	10
CAAGACG	450	0.0	12.453979	4
CGGACCA	420	0.0	12.442006	9
CGCAAGA	415	0.0	12.35876	2
GGTATCA	850	0.0	12.288099	1
TCGCGTA	195	0.0	12.180985	9
AAGACGG	470	0.0	12.127775	5
CGAGCCG	330	0.0	11.804215	15
CGAACGA	125	1.8328137E-8	11.400109	16
AGACGGA	520	0.0	11.327802	6
GCTCGTA	160	4.5474735E-11	11.282638	9
ACGAACG	135	4.7239155E-9	11.260389	15
GCGTAAC	220	0.0	11.229419	11
GACGGAC	530	0.0	11.114071	7
CGTAGAC	60	0.0058855005	11.081177	3
ACGGTAT	275	0.0	11.055905	9
CGGTCGG	155	3.1286618E-10	11.034368	10
CGGTCCA	310	0.0	11.034368	10
>>END_MODULE