FastQCFastQC Report
Thu 2 Feb 2017
SRR4062101_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062101_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2207140
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA23500.026.8769131
GGTATCA10650.025.6234421
CGCAATA3650.025.3159336
GCGCAAT4100.023.07343335
CAATACG4050.022.81610738
CTAGCGG4150.022.7938929
CGAATGC4350.020.73915543
TACGAAT4400.020.50162741
AATACGA4600.020.08854939
TCTAGCG4750.019.91511328
TAGCGGC4850.019.50405130
ATACGAA4750.019.45417440
ATACCGT4050.018.4680886
GTCGTAT851.427913E-418.1236021
TCAACGC34050.017.7669814
ATCAACG34750.017.5990033
CAACGCA35150.017.2109745
CGCCGGT5150.017.0864127
AACGCAG35950.016.8891686
TATCAAC37050.016.685752
Produced by FastQC (version 0.11.5)