##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062101_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2207140
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.265638790470927	32.0	32.0	32.0	32.0	32.0
2	31.40108466159827	32.0	32.0	32.0	32.0	32.0
3	31.54324646374947	32.0	32.0	32.0	32.0	32.0
4	31.61943284068976	32.0	32.0	32.0	32.0	32.0
5	31.593819603649973	32.0	32.0	32.0	32.0	32.0
6	35.22926320940221	36.0	36.0	36.0	36.0	36.0
7	35.22951783756354	36.0	36.0	36.0	36.0	36.0
8	35.17170682421595	36.0	36.0	36.0	36.0	36.0
9	35.271687795065105	36.0	36.0	36.0	36.0	36.0
10	35.137305744085104	36.0	36.0	36.0	36.0	36.0
11	35.27325316925977	36.0	36.0	36.0	36.0	36.0
12	35.18732069556077	36.0	36.0	36.0	36.0	36.0
13	35.22555207191207	36.0	36.0	36.0	36.0	36.0
14	35.18837771958281	36.0	36.0	36.0	36.0	36.0
15	35.165552253142074	36.0	36.0	36.0	36.0	36.0
16	35.180567159310236	36.0	36.0	36.0	36.0	36.0
17	35.162975162427394	36.0	36.0	36.0	36.0	36.0
18	35.154133403408935	36.0	36.0	36.0	36.0	36.0
19	35.1505998713267	36.0	36.0	36.0	36.0	36.0
20	35.14936025807153	36.0	36.0	36.0	36.0	36.0
21	35.140922188896035	36.0	36.0	36.0	36.0	36.0
22	35.12641925750066	36.0	36.0	36.0	36.0	36.0
23	35.09526853756445	36.0	36.0	36.0	36.0	36.0
24	35.08050282265737	36.0	36.0	36.0	36.0	36.0
25	35.06416448435532	36.0	36.0	36.0	36.0	36.0
26	35.02722890256169	36.0	36.0	36.0	36.0	36.0
27	35.009924608316645	36.0	36.0	36.0	36.0	36.0
28	34.99502750165372	36.0	36.0	36.0	36.0	36.0
29	34.96918364942867	36.0	36.0	36.0	36.0	36.0
30	34.95620169087598	36.0	36.0	36.0	36.0	36.0
31	34.95702447511259	36.0	36.0	36.0	36.0	36.0
32	34.9381842565492	36.0	36.0	36.0	36.0	36.0
33	34.91186331632792	36.0	36.0	36.0	32.0	36.0
34	34.89859727973758	36.0	36.0	36.0	32.0	36.0
35	34.8903572043459	36.0	36.0	36.0	32.0	36.0
36	34.86809989398044	36.0	36.0	36.0	32.0	36.0
37	34.86195800900713	36.0	36.0	36.0	32.0	36.0
38	34.84692407368812	36.0	36.0	36.0	32.0	36.0
39	34.834894478827806	36.0	36.0	36.0	32.0	36.0
40	34.81891950669192	36.0	36.0	36.0	32.0	36.0
41	34.793972289931766	36.0	36.0	36.0	32.0	36.0
42	34.76791413322218	36.0	36.0	36.0	32.0	36.0
43	34.76709225513561	36.0	36.0	36.0	32.0	36.0
44	34.720288246327826	36.0	36.0	36.0	32.0	36.0
45	34.70878920231612	36.0	36.0	36.0	32.0	36.0
46	34.70102304339552	36.0	36.0	36.0	32.0	36.0
47	34.679299908478846	36.0	36.0	36.0	32.0	36.0
48	34.66038402638709	36.0	36.0	36.0	32.0	36.0
49	34.648814302672235	36.0	36.0	36.0	32.0	36.0
50	34.224673106372954	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	2.0
19	3.0
20	11.0
21	57.0
22	125.0
23	382.0
24	1005.0
25	2489.0
26	5196.0
27	9840.0
28	17320.0
29	27653.0
30	41782.0
31	63027.0
32	95239.0
33	162347.0
34	368097.0
35	1412565.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.00312899537222	17.690154224483663	11.921680847004183	26.385035933139932
2	16.29527263830144	19.589133887165872	36.97772360781044	27.137869866722248
3	18.79167172602273	22.79754903648717	28.276333268694398	30.134445968795703
4	12.627840838297905	15.721755630252558	35.16955660699213	36.48084692445741
5	14.752783697968772	36.485699114146115	33.27502247256173	15.486494715323387
6	35.5572399900867	34.8784420722411	16.059084687538654	13.505233250133545
7	31.13635745806791	30.004485442699604	19.825566117237692	19.0335909819948
8	28.187065614324418	33.69509863443189	18.651105049974177	19.466730701269515
9	26.77410585644771	14.615520537890664	18.450120971030383	40.16025263463124
10	15.81961987894736	27.232494192708295	31.372695602769014	25.575190325575324
11	37.523356017289345	21.37689498627183	21.544079668711547	19.555669327727284
12	24.251655989198692	23.693694101869387	28.682820301385505	23.371829607546417
13	29.391520247922653	19.50356570040867	25.045805884538364	26.05910816713031
14	23.264541442772092	19.40946201872106	25.11644028018159	32.20955625832526
15	25.06478972788314	27.379867158404085	21.895031579328904	25.66031153438386
16	25.68137282664099	26.268833890241833	23.43591776632497	24.61387551679221
17	24.14409597941227	26.32769103908225	24.981650461683447	24.546562519822032
18	24.82583796224979	25.599644789184197	25.624971682811235	23.94954556575478
19	25.32372210190563	25.450537800048934	25.196272098734106	24.029467999311326
20	25.606817175781593	24.756437202866525	24.884975110222275	24.751770511129607
21	26.219949799287768	24.646737406779813	24.758239169241644	24.375073624690778
22	25.569469992841416	24.67858858069719	25.12550178058483	24.626439645876562
23	24.45291191315458	24.758012631731564	25.527968320994592	25.261107134119264
24	24.57114947481357	25.240981350818554	25.32964889555217	24.858220278815708
25	24.70776608238006	24.80635538544068	25.291690226610413	25.194188305568844
26	24.6177852203036	25.562131196265202	25.366764893509053	24.453318689922142
27	25.070849959381626	25.094002208298654	25.045160477706556	24.789987354613167
28	24.717390102695568	24.968850612521557	25.49524127975729	24.818518005025584
29	24.60593621580967	25.30816037116074	25.41055578964538	24.675347623384212
30	24.587504876270717	25.365352176617733	25.51033769392379	24.536805253187755
31	24.96710657469798	25.229030667908702	24.86516414091072	24.938698616482597
32	24.986679473703287	25.171490449092033	24.770379499075716	25.07145057812896
33	24.546074435863687	24.99597895008688	25.188989345916767	25.26895726813267
34	25.030039200477	25.146390130831364	25.403467692681307	24.420102976010323
35	25.384536270710633	24.915920565039176	25.41602499887864	24.28351816537155
36	24.499033134402474	25.435361364884386	25.135379306720214	24.930226193992926
37	25.347912389736184	25.164095939772363	24.98757415296954	24.500417517521917
38	24.656263153340138	24.930600202343395	25.41733891703241	24.995797727284057
39	25.222212858578473	25.04274805424993	24.932513803068073	24.802525284103524
40	25.306969792115495	25.036438575005924	25.203804563283054	24.452787069595527
41	24.481903080762407	25.56612339360505	25.38729273857831	24.56468078705424
42	25.45306259302639	25.282562351752464	25.13530596478987	24.12906909043128
43	25.17201788097894	24.668401722127122	25.18982455218261	24.96975584471133
44	24.658671331744777	25.230297453101148	25.12753524295243	24.983495972201645
45	24.876771292321283	25.34304452725893	25.071644597487623	24.70853958293216
46	24.747086190054173	25.172445353037233	25.068008532556195	25.012459924352402
47	24.894206024140058	25.049249700967778	25.394450686867952	24.662093588024213
48	25.312011367204473	25.571407276043917	24.6281576637785	24.488423692973114
49	24.70586422927525	25.547119492501107	24.903049714574916	24.843966563648728
50	24.94533545859767	25.56886916666138	24.858841791347732	24.626953583393217
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	26.0
1	35.0
2	44.0
3	104.5
4	165.0
5	188.0
6	211.0
7	175.0
8	139.0
9	204.5
10	270.0
11	481.5
12	693.0
13	1204.0
14	1715.0
15	2280.0
16	2845.0
17	3011.0
18	3177.0
19	3443.5
20	3710.0
21	4843.5
22	5977.0
23	7495.5
24	9014.0
25	11410.5
26	13807.0
27	17212.5
28	20618.0
29	24293.5
30	27969.0
31	32280.5
32	36592.0
33	41665.5
34	46739.0
35	53947.5
36	61156.0
37	71197.5
38	81239.0
39	89336.5
40	97434.0
41	106638.0
42	115842.0
43	122035.0
44	128228.0
45	137952.0
46	147676.0
47	156473.5
48	165271.0
49	170329.0
50	175387.0
51	172647.5
52	169908.0
53	167890.0
54	165872.0
55	164122.0
56	162372.0
57	154810.0
58	147248.0
59	133926.0
60	120604.0
61	106076.0
62	91548.0
63	78917.0
64	66286.0
65	55761.5
66	45237.0
67	38418.5
68	31600.0
69	27531.5
70	23463.0
71	18370.5
72	13278.0
73	11622.0
74	9966.0
75	7352.0
76	4738.0
77	4100.5
78	3463.0
79	2970.0
80	2477.0
81	1922.0
82	1367.0
83	1148.5
84	930.0
85	729.5
86	529.0
87	347.5
88	166.0
89	106.0
90	46.0
91	35.5
92	25.0
93	28.0
94	31.0
95	23.5
96	16.0
97	17.5
98	19.0
99	13.0
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.030854408873021193
2	0.004304212691537464
3	3.6246001612947075E-4
4	4.5307502016183843E-5
5	1.8123000806473537E-4
6	1.3592250604855152E-4
7	0.0
8	0.0
9	0.0
10	4.5307502016183843E-5
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.796125302427576E-4
17	0.0
18	0.0
19	0.0
20	4.0776751814565454E-4
21	0.0
22	0.0
23	0.0
24	8.60842538307493E-4
25	1.8123000806473537E-4
26	1.8123000806473537E-4
27	4.983825221780223E-4
28	0.0015404550685502506
29	4.530750201618384E-4
30	0.0010420725463722283
31	5.436900241942061E-4
32	9.061500403236768E-4
33	2.265375100809192E-4
34	0.0010873800483884122
35	4.5307502016183843E-5
36	3.6246001612947075E-4
37	0.0021294525947606405
38	4.5307502016183843E-5
39	9.967650443560446E-4
40	4.5307502016183843E-5
41	0.0010873800483884122
42	0.0047572877116993035
43	0.004621365205650751
44	0.004666672707666936
45	0.0029449876310519494
46	0.002899680129035766
47	9.061500403236768E-4
48	0.004032367679440362
49	0.003669907663310891
50	0.0019029150846797212
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	2207140.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.391924418572195
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.7710975368227	34.65507949257826
2	16.635120149232247	16.765514345079577
3	6.539586787533233	9.886270893782042
4	3.0806526914053602	6.209600703406797
5	1.5760629444377128	3.9710422387508784
6	0.922864242018118	2.7902943079427955
7	0.5734084767532688	2.022660963506353
8	0.38872785944698535	1.5670995930116676
9	0.26801414358114406	1.2155173619804434
>10	1.1131462510775487	9.521014300982406
>50	0.07001471092661041	2.4618015942837164
>100	0.05333934322737568	5.462937207556388
>500	0.006064157466158989	2.1182027393519607
>1k	0.0019007060714826027	1.3529642577868084
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	9.061500403236767E-5
2	0.0	0.0	0.0	0.0	9.061500403236767E-5
3	0.0	0.0	0.0	4.530750201618384E-5	9.061500403236767E-5
4	0.0	0.0	0.0	4.530750201618384E-5	1.3592250604855152E-4
5	0.0	0.0	0.0	4.530750201618384E-5	1.3592250604855152E-4
6	0.0	0.0	0.0	4.530750201618384E-5	1.3592250604855152E-4
7	0.0	0.0	0.0	4.530750201618384E-5	1.3592250604855152E-4
8	4.530750201618384E-5	0.0	0.0	4.530750201618384E-5	1.3592250604855152E-4
9	4.530750201618384E-5	0.0	0.0	4.530750201618384E-5	1.8123000806473535E-4
10	4.530750201618384E-5	0.0	0.0	4.530750201618384E-5	1.8123000806473535E-4
11	4.530750201618384E-5	0.0	0.0	1.3592250604855152E-4	1.8123000806473535E-4
12	4.530750201618384E-5	0.0	0.0	1.3592250604855152E-4	3.171525141132869E-4
13	4.530750201618384E-5	0.0	0.0	1.3592250604855152E-4	3.624600161294707E-4
14	4.530750201618384E-5	0.0	0.0	1.3592250604855152E-4	3.624600161294707E-4
15	4.530750201618384E-5	0.0	0.0	1.3592250604855152E-4	6.343050282265738E-4
16	4.530750201618384E-5	0.0	0.0	1.3592250604855152E-4	6.796125302427576E-4
17	4.530750201618384E-5	0.0	0.0	1.3592250604855152E-4	6.796125302427576E-4
18	4.530750201618384E-5	0.0	0.0	1.3592250604855152E-4	7.249200322589414E-4
19	1.3592250604855152E-4	0.0	0.0	1.3592250604855152E-4	7.249200322589414E-4
20	1.3592250604855152E-4	0.0	0.0	1.3592250604855152E-4	9.061500403236768E-4
21	2.7184501209710305E-4	0.0	0.0	1.3592250604855152E-4	9.967650443560444E-4
22	3.171525141132869E-4	0.0	0.0	1.8123000806473535E-4	9.967650443560444E-4
23	3.171525141132869E-4	0.0	0.0	1.8123000806473535E-4	0.0010420725463722283
24	3.624600161294707E-4	0.0	0.0	2.7184501209710305E-4	0.0010873800483884122
25	3.624600161294707E-4	0.0	0.0	3.171525141132869E-4	0.0010873800483884122
26	3.624600161294707E-4	0.0	0.0	4.0776751814565454E-4	0.0012233025544369637
27	3.624600161294707E-4	0.0	0.0	5.436900241942061E-4	0.0012233025544369637
28	3.624600161294707E-4	0.0	0.0	6.796125302427576E-4	0.0012686100564531476
29	3.624600161294707E-4	0.0	0.0	9.967650443560444E-4	0.0012686100564531476
30	3.624600161294707E-4	0.0	0.0	0.0017669925786311697	0.0012686100564531476
31	3.624600161294707E-4	0.0	0.0	0.003443370153229972	0.0012686100564531476
32	3.624600161294707E-4	0.0	0.0	0.00566343775202298	0.0013139175584693313
33	3.624600161294707E-4	0.0	0.0	0.0077022753427512525	0.0013592250604855152
34	3.624600161294707E-4	0.0	0.0	0.009741112933479526	0.0014498400645178828
35	3.624600161294707E-4	0.0	0.0	0.012686100564531476	0.0014498400645178828
36	3.624600161294707E-4	0.0	0.0	0.016673160741955653	0.0014498400645178828
37	4.0776751814565454E-4	0.0	0.0	0.022834981016156657	0.0014498400645178828
38	4.0776751814565454E-4	0.0	0.0	0.033119783973830384	0.0014498400645178828
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2350	0.0	26.876913	1
GGTATCA	1065	0.0	25.623442	1
CGCAATA	365	0.0	25.31593	36
GCGCAAT	410	0.0	23.073433	35
CAATACG	405	0.0	22.816107	38
CTAGCGG	415	0.0	22.79389	29
CGAATGC	435	0.0	20.739155	43
TACGAAT	440	0.0	20.501627	41
AATACGA	460	0.0	20.088549	39
TCTAGCG	475	0.0	19.915113	28
TAGCGGC	485	0.0	19.504051	30
ATACGAA	475	0.0	19.454174	40
ATACCGT	405	0.0	18.468088	6
GTCGTAT	85	1.427913E-4	18.123602	1
TCAACGC	3405	0.0	17.766981	4
ATCAACG	3475	0.0	17.599003	3
CAACGCA	3515	0.0	17.210974	5
CGCCGGT	515	0.0	17.086412	7
AACGCAG	3595	0.0	16.889168	6
TATCAAC	3705	0.0	16.68575	2
>>END_MODULE