FastQCFastQC Report
Thu 2 Feb 2017
SRR4062100_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062100_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956257
Sequences flagged as poor quality0
Sequence length25
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT16140.16878307818923155No Hit
TATCAACGCAGAGTACTTTTTTTTT10290.10760705542547662No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA3100.014.396521
CGAACGA551.9588144E-413.81825816
TTGCGCG500.001499929913.2986818
GTATCAA9500.013.1938541
CGCATCG957.369454E-813.00075213
CCGACCA1700.012.8543589
ACGGTAT1207.366907E-1012.6680629
ACGAACG604.087463E-412.667415
TTTGCGC604.0892692E-412.66673717
CTTACAC905.404145E-712.665413
GTAACAC1251.4097168E-912.1587933
TAGGACA550.00306682912.0909774
ATTTGCG550.00306682912.09097716
CGCAATA550.003071643912.0884442
CGACCAT1750.011.94417410
AATTCCG802.8634875E-511.8750665
AACGAAC658.014556E-411.69298514
TAGGCAG658.018078E-411.6923725
AACCGCG752.0700968E-411.40125757
TAATTCC1251.8273568E-811.40006354
Produced by FastQC (version 0.11.5)