FastQCFastQC Report
Thu 2 Feb 2017
SRR4062100_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062100_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences956257
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA4200.030.3875261
GTATCAA10450.026.3214821
GTATAGG1303.576133E-818.6194111
AATCGGA600.007412927718.33250616
TGCACGA851.430359E-418.1177772
CGCGTAA1751.9099389E-1017.59920510
TCGCGTA1751.9099389E-1017.5992059
GGCGTTA3150.017.4631842
TAGGATA902.2155265E-417.1103384
GCGTAAC1802.8558134E-1017.11033811
CTAGGCC1753.623427E-916.342123
TCAACGC15950.016.2751274
CGGTCCA1906.166374E-1016.20979510
CAACGCA16400.015.8285535
ATCAACG16500.015.7326223
GCGTTAT3500.015.71604243
ATACTGT2101.5097612E-1015.7135756
TATCAAC16900.015.6214122
GTTCTAG1552.6589805E-715.61628151
CGACCAT3400.015.5287110
Produced by FastQC (version 0.11.5)