FastQCFastQC Report
Thu 2 Feb 2017
SRR4062099_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062099_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783055
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGC28150.15787510761025317No Hit
GTATCAACGCAGAGTACTTTTTTTT27950.15675343721870608No Hit
GAATAGGACCGCGGTTCTATTTTGT23060.1293285961453797No Hit
GATTAAGAGGGACGGCCGGGGGCAT22780.1277582575972138No Hit
GGGTAGGCACACGCTGAGCCAGTCA22000.1233837430701801No Hit
ATCAGATACCGTCGTAGTTCCGACC21910.12287899139398392No Hit
TCGTAGTTCCGACCATAAACGATGC21280.11934572966061058No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20840.11687805479920699No Hit
GTATCTGATCGTCTTCGAACCTCCG20660.1158685514468146No Hit
GAACTACGACGGTATCTGATCGTCT20640.1157563844076599No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20560.11530771625104105No Hit
GAATAACGCCGCCGCATCGCCAGTC19510.10941894669541882No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC19340.1084655268626038No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT19180.10756819054936612No Hit
ACCATACTCCCCCCGGAACCCAAAG17910.10044558356304208No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTCCA3600.013.46018210
GGTATCA6700.013.1820871
ACTGCGC655.449204E-513.1549158
GTATCAA19900.013.1236811
TAGGTTG604.0916796E-412.66698555
GCTCGTA2050.012.5135639
CGGAATT1405.2750693E-1112.21527915
CAAGACG5100.011.9211984
AAGACGG4900.011.8268285
AGCCCCG3950.011.78510716
AACCGCG1950.011.6935857
CGAACGA1302.6138878E-911.69260116
CCCTTAT658.047341E-411.6883341
GCGTTAT2450.011.6287011
CGCAAGA5100.011.5463912
AGGCCCG4450.011.52967510
CCGGTCC4300.011.4896299
GTACTAA1001.937815E-611.3961271
CGCATCG3700.011.29789813
CGAGCCG4650.011.237473515
Produced by FastQC (version 0.11.5)