##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062099_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1783055
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.289930484477484	32.0	32.0	32.0	32.0	32.0
2	30.861154591417538	32.0	32.0	32.0	32.0	32.0
3	30.884854925955732	32.0	32.0	32.0	32.0	32.0
4	30.896274652212075	32.0	32.0	32.0	32.0	32.0
5	30.826672761075795	32.0	32.0	32.0	32.0	32.0
6	34.464512872569834	36.0	36.0	36.0	32.0	36.0
7	34.4053610236364	36.0	36.0	36.0	32.0	36.0
8	34.37715662164095	36.0	36.0	36.0	32.0	36.0
9	34.48975830807238	36.0	36.0	36.0	32.0	36.0
10	34.21460807434431	36.0	36.0	36.0	32.0	36.0
11	34.470662991326684	36.0	36.0	36.0	32.0	36.0
12	34.30509883318238	36.0	36.0	36.0	32.0	36.0
13	34.39689689886178	36.0	36.0	36.0	32.0	36.0
14	34.30733432227273	36.0	36.0	36.0	32.0	36.0
15	34.26208669951291	36.0	36.0	36.0	32.0	36.0
16	34.26537038958417	36.0	36.0	36.0	32.0	36.0
17	34.19379435855877	36.0	36.0	36.0	32.0	36.0
18	34.19694400901823	36.0	36.0	36.0	32.0	36.0
19	34.19851995591836	36.0	36.0	36.0	32.0	36.0
20	34.194607569592634	36.0	36.0	36.0	32.0	36.0
21	34.170365468255326	36.0	36.0	36.0	32.0	36.0
22	34.14181110509771	36.0	36.0	36.0	32.0	36.0
23	34.11826107439199	36.0	36.0	36.0	32.0	36.0
24	34.09079809652535	36.0	36.0	36.0	32.0	36.0
25	33.74719680548272	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	3.0
4	27.0
5	138.0
6	350.0
7	69.0
8	308.0
9	233.0
10	135.0
11	42.0
12	75.0
13	89.0
14	221.0
15	295.0
16	534.0
17	728.0
18	1043.0
19	1415.0
20	2138.0
21	3089.0
22	4563.0
23	6721.0
24	9592.0
25	13722.0
26	18902.0
27	25012.0
28	33298.0
29	44830.0
30	58779.0
31	81658.0
32	115697.0
33	165730.0
34	370531.0
35	823088.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.08417655708964	17.207765622493742	11.440025394859784	26.268032425556832
2	16.929334125233517	19.32494435106528	36.710686670558864	27.035034853142335
3	19.115154628652316	22.793410826192932	28.107182876942044	29.984251668212707
4	12.692231324857897	15.215118476979356	35.21054160744594	36.882108590716804
5	15.20374627902306	36.043461235078226	33.05018564210626	15.702606843792463
6	35.1974876735053	35.02727934929128	16.21805296941413	13.55718000778929
7	30.97804606374187	29.960689069742376	20.142163864036505	18.91910100247925
8	28.5539114543418	32.67835035918512	18.946502612127798	19.821235574345284
9	27.08739374153877	14.092290657004227	18.532306671226113	40.28800893023089
10	16.385749754163772	26.230992268584014	30.64801362643114	26.735244350821073
11	37.73001466365419	21.036194121845924	21.77218488447388	19.461606330026008
12	24.68483879156006	23.424081640904916	28.23787146005225	23.653208107482776
13	29.082980957675613	19.231119122626726	25.22801933994845	26.45788057974921
14	23.92929735314265	19.44788824130535	24.527619625150198	32.095194780401805
15	25.35374914851427	27.065144203635516	21.76113923034114	25.81996741750907
16	26.185569673134502	25.632258904968	23.169537473974067	25.01263394792343
17	24.300784119062115	25.907711589733452	24.72131316920216	25.070191122002278
18	25.190926700541816	24.765503495129277	25.677718199938237	24.365851604390667
19	25.681505720780123	24.873786920097704	24.957264669375903	24.48744268974627
20	25.926029879500756	24.152559023072577	24.437668334622565	25.483742762804095
21	26.982269181068112	24.06891588610871	24.091426043747173	24.857388889076006
22	26.27026632533908	23.978599658672415	24.75775846582233	24.99337555016617
23	24.516021483588986	24.142152254188417	25.29210462950126	26.04972163272133
24	24.870135629259813	24.75562371384595	24.98857687510455	25.385663781789685
25	24.985658072736864	24.274118855564726	24.905107522354882	25.83511554934353
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	286.0
1	286.0
2	395.0
3	504.0
4	504.0
5	504.0
6	1178.0
7	1852.0
8	1852.0
9	1852.0
10	2198.0
11	2544.0
12	2544.0
13	2544.0
14	3582.5
15	4621.0
16	4621.0
17	4621.0
18	7672.5
19	10724.0
20	10724.0
21	10724.0
22	17123.5
23	23523.0
24	23523.0
25	23523.0
26	34819.0
27	46115.0
28	46115.0
29	46115.0
30	57419.5
31	68724.0
32	68724.0
33	68724.0
34	86669.5
35	104615.0
36	104615.0
37	104615.0
38	122189.5
39	139764.0
40	139764.0
41	139764.0
42	161117.5
43	182471.0
44	182471.0
45	182471.0
46	204183.5
47	225896.0
48	225896.0
49	225896.0
50	234614.5
51	243333.0
52	243333.0
53	243333.0
54	227545.0
55	211757.0
56	211757.0
57	211757.0
58	196805.0
59	181853.0
60	181853.0
61	181853.0
62	161926.5
63	142000.0
64	142000.0
65	142000.0
66	117401.5
67	92803.0
68	92803.0
69	92803.0
70	70242.5
71	47682.0
72	47682.0
73	47682.0
74	36436.0
75	25190.0
76	25190.0
77	25190.0
78	20578.0
79	15966.0
80	15966.0
81	15966.0
82	11094.0
83	6222.0
84	6222.0
85	6222.0
86	4541.0
87	2860.0
88	2860.0
89	2860.0
90	1869.0
91	878.0
92	878.0
93	878.0
94	526.5
95	175.0
96	175.0
97	175.0
98	436.0
99	697.0
100	697.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	9.534198328150281E-4
2	1.1216703915470919E-4
3	5.047516761961914E-4
4	0.009926782965191764
5	0.03325752710937127
6	0.06286962544621451
7	0.10493226512923044
8	0.10880202798006793
9	0.11990656485638414
10	0.13448827994649631
11	0.13807762519944702
12	0.1334226930745266
13	0.12815084223425527
14	0.11637330312301078
15	0.13145976988931918
16	0.11996264837596149
17	0.12355199362891217
18	0.11244745675259597
19	0.09769749110375171
20	0.09158438746982006
21	0.09180872154812947
22	0.09954824724980442
23	0.07941426372153411
24	0.08866804445179761
25	0.08777070813855994
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	1783055.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	49.18761697964735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.57607052878556	37.665944269763656
2	13.555156492417192	13.334916912963937
3	4.226215058118359	6.236323426570318
4	1.7516447917796039	3.446369324097972
5	0.949846859877301	2.3360351766482723
6	0.5952480392924855	1.7567299538762915
7	0.3916577526163634	1.348529806596221
8	0.2872661065851347	1.1303948177555323
9	0.21979697496439723	0.9730160476050518
>10	1.2548534729577865	11.164838817364194
>50	0.0929522936720349	3.1941239099334924
>100	0.07925062474537306	8.17809131609428
>500	0.013742402872079049	4.513414867854406
>1k	0.006298601316369563	4.721271352876344
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGC	2815	0.15787510761025317	No Hit
GTATCAACGCAGAGTACTTTTTTTT	2795	0.15675343721870608	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2306	0.1293285961453797	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	2278	0.1277582575972138	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	2200	0.1233837430701801	No Hit
ATCAGATACCGTCGTAGTTCCGACC	2191	0.12287899139398392	No Hit
TCGTAGTTCCGACCATAAACGATGC	2128	0.11934572966061058	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2084	0.11687805479920699	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2066	0.1158685514468146	No Hit
GAACTACGACGGTATCTGATCGTCT	2064	0.1157563844076599	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2056	0.11530771625104105	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1951	0.10941894669541882	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1934	0.1084655268626038	No Hit
CTCCTGGTGGTGCCCTTCCGTCAAT	1918	0.10756819054936612	No Hit
ACCATACTCCCCCCGGAACCCAAAG	1791	0.10044558356304208	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	2.80417597886773E-4
2	0.0	0.0	0.0	0.0	2.80417597886773E-4
3	0.0	0.0	0.0	0.0	2.80417597886773E-4
4	0.0	0.0	0.0	0.0	2.80417597886773E-4
5	0.0	0.0	0.0	0.0	2.80417597886773E-4
6	5.6083519577354595E-5	0.0	0.0	0.0	2.80417597886773E-4
7	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	2.80417597886773E-4
8	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	2.80417597886773E-4
9	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	2.80417597886773E-4
10	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	2.80417597886773E-4
11	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	2.80417597886773E-4
12	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	6.169187153509005E-4
13	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	7.290857545056098E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGTCCA	360	0.0	13.460182	10
GGTATCA	670	0.0	13.182087	1
ACTGCGC	65	5.449204E-5	13.154915	8
GTATCAA	1990	0.0	13.123681	1
TAGGTTG	60	4.0916796E-4	12.6669855	5
GCTCGTA	205	0.0	12.513563	9
CGGAATT	140	5.2750693E-11	12.215279	15
CAAGACG	510	0.0	11.921198	4
AAGACGG	490	0.0	11.826828	5
AGCCCCG	395	0.0	11.785107	16
AACCGCG	195	0.0	11.693585	7
CGAACGA	130	2.6138878E-9	11.692601	16
CCCTTAT	65	8.047341E-4	11.688334	1
GCGTTAT	245	0.0	11.628701	1
CGCAAGA	510	0.0	11.546391	2
AGGCCCG	445	0.0	11.529675	10
CCGGTCC	430	0.0	11.489629	9
GTACTAA	100	1.937815E-6	11.396127	1
CGCATCG	370	0.0	11.297898	13
CGAGCCG	465	0.0	11.2374735	15
>>END_MODULE