FastQCFastQC Report
Thu 2 Feb 2017
SRR4062099_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062099_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1783055
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT27410.15372492716152897No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA25560.14334947603971837No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT23890.13398352827030013No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG23420.13134760285016447No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT23000.12899209502791556No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA22750.1275900070384817No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC21970.12321549251144805No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA20170.11312045898752422No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC19460.10913852909753205No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC19400.10880202798006793No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA18850.10571743440331341No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG18050.10123075283712504No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA20550.030.6273731
GGTATCA7600.024.0336691
CGTCGTA7550.019.52258110
TCAACGC32200.019.4031314
ATACCGT7600.019.3941446
ATCAACG32250.019.373053
CAACGCA32500.019.2240285
CCGTCGT7500.019.0660849
TACCGTC7800.018.8968587
ACCGTCG8000.018.6994298
GTCCTAA2950.018.6497751
AACGCAG33950.018.4029716
GTCCTAT5300.018.2697411
GTATATA2300.018.1794761
TATCAAC35250.018.0363272
TACGACG6900.017.8545275
GTCGTAG8050.017.76343211
CGTCTTA1408.3815394E-817.28518515
CGACGGT6950.017.0930027
ATTACAC3350.017.0741043
Produced by FastQC (version 0.11.5)