##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062099_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1783055
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.29753092305061	32.0	32.0	32.0	32.0	32.0
2	31.44806413711299	32.0	32.0	32.0	32.0	32.0
3	31.571618374082686	32.0	32.0	32.0	32.0	32.0
4	31.646201603427826	32.0	32.0	32.0	32.0	32.0
5	31.61697311636489	32.0	32.0	32.0	32.0	32.0
6	35.2554267815631	36.0	36.0	36.0	36.0	36.0
7	35.256239992596974	36.0	36.0	36.0	36.0	36.0
8	35.208396263715926	36.0	36.0	36.0	36.0	36.0
9	35.29597011870077	36.0	36.0	36.0	36.0	36.0
10	35.16740033257527	36.0	36.0	36.0	36.0	36.0
11	35.298886461718794	36.0	36.0	36.0	36.0	36.0
12	35.20737386115403	36.0	36.0	36.0	36.0	36.0
13	35.25211280639128	36.0	36.0	36.0	36.0	36.0
14	35.212402309519334	36.0	36.0	36.0	36.0	36.0
15	35.19044841578078	36.0	36.0	36.0	36.0	36.0
16	35.200661224695814	36.0	36.0	36.0	36.0	36.0
17	35.178392141577234	36.0	36.0	36.0	36.0	36.0
18	35.174279537086626	36.0	36.0	36.0	36.0	36.0
19	35.16949056534992	36.0	36.0	36.0	36.0	36.0
20	35.16989829253725	36.0	36.0	36.0	36.0	36.0
21	35.16222270204789	36.0	36.0	36.0	36.0	36.0
22	35.14499160149294	36.0	36.0	36.0	36.0	36.0
23	35.10974423110897	36.0	36.0	36.0	36.0	36.0
24	35.09153727731338	36.0	36.0	36.0	36.0	36.0
25	35.07301176912658	36.0	36.0	36.0	36.0	36.0
26	35.03757259310565	36.0	36.0	36.0	36.0	36.0
27	35.02009640757015	36.0	36.0	36.0	36.0	36.0
28	35.00046100653093	36.0	36.0	36.0	36.0	36.0
29	34.97771128764957	36.0	36.0	36.0	36.0	36.0
30	34.95587124345575	36.0	36.0	36.0	36.0	36.0
31	34.969730041978515	36.0	36.0	36.0	36.0	36.0
32	34.93843712055994	36.0	36.0	36.0	32.0	36.0
33	34.913494536063105	36.0	36.0	36.0	32.0	36.0
34	34.909508680326745	36.0	36.0	36.0	32.0	36.0
35	34.89494154695172	36.0	36.0	36.0	32.0	36.0
36	34.87029732677904	36.0	36.0	36.0	32.0	36.0
37	34.85864934059802	36.0	36.0	36.0	32.0	36.0
38	34.838914110893946	36.0	36.0	36.0	32.0	36.0
39	34.82666939606462	36.0	36.0	36.0	32.0	36.0
40	34.81081907176167	36.0	36.0	36.0	32.0	36.0
41	34.78902725939469	36.0	36.0	36.0	32.0	36.0
42	34.740728132334674	36.0	36.0	36.0	32.0	36.0
43	34.760344464977244	36.0	36.0	36.0	32.0	36.0
44	34.6994820686967	36.0	36.0	36.0	32.0	36.0
45	34.68938310932641	36.0	36.0	36.0	32.0	36.0
46	34.66397615328748	36.0	36.0	36.0	32.0	36.0
47	34.65028784866423	36.0	36.0	36.0	32.0	36.0
48	34.62151868562663	36.0	36.0	36.0	32.0	36.0
49	34.61474099228571	36.0	36.0	36.0	32.0	36.0
50	34.19723283914405	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	4.0
20	15.0
21	58.0
22	112.0
23	285.0
24	828.0
25	1963.0
26	4221.0
27	8175.0
28	13828.0
29	22512.0
30	33419.0
31	49769.0
32	75943.0
33	129351.0
34	294648.0
35	1147924.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.98141914635788	17.72530407073291	11.921928997800817	26.37134778510839
2	16.44022851707316	19.86114211424283	36.4859498792467	27.212679489437313
3	18.392514831361613	23.30601678251711	28.4788502371784	29.822618148942876
4	12.532542480485729	15.65280692564555	35.472902110648356	36.34174848322036
5	14.88142541873358	36.44245410264967	32.879019435743714	15.797101042873047
6	35.22456732869671	35.29545705647231	16.072114594590957	13.407861020240027
7	30.98659323464503	30.047699033400537	20.159894114315037	18.805813617639387
8	28.539669275485053	32.7609075435138	18.860046381070692	19.839376799930456
9	27.227595334972843	13.892785135623972	18.410761305736504	40.468858223666686
10	16.334343398653882	26.314865570456963	30.66201621600704	26.688774814882116
11	37.96237356671555	20.921564393695093	21.731242165833358	19.384819873755998
12	24.694358839183312	23.441677345903518	28.304791495495092	23.559172319418078
13	29.237348259027346	19.114329058834418	25.242238741934486	26.40608394020375
14	23.91591958744963	19.350328509215924	24.541643415374175	32.19210848796027
15	25.373586344784655	27.08323635558073	21.726418983149706	25.81675831648491
16	26.219611709618423	25.680534500773117	23.149586073726606	24.950267715881857
17	24.390442246593626	25.895779995569402	24.670972011519552	25.042805746317416
18	25.291662507136632	24.683772897511798	25.69361331737569	24.330951277975878
19	25.88114219695971	24.69306891823303	25.001640442947636	24.424148441859618
20	26.153949692941868	23.957432489273998	24.404138975351223	25.48447884243291
21	27.24946790760801	23.985631402284284	24.034031479679538	24.730869210428168
22	26.369349234880584	23.941942340533522	24.81420932052012	24.874499104065777
23	24.60933622350404	23.96639475506925	25.323896346439117	26.100372674987593
24	25.039413091259465	24.69152350736412	25.033692514513163	25.235370886863258
25	25.192745252104114	24.102087823525157	25.008454600059558	25.696712324311164
26	24.817405671514724	25.313745933234667	25.338534904497966	24.530313490752647
27	25.510249864277352	24.419308958592254	24.79714511331159	25.273296063818805
28	25.01465198383655	24.269069311595118	25.465512454887314	25.25076624968102
29	24.75223591974861	24.560149003363343	25.409649885841485	25.277965191046565
30	24.77731876198093	24.763466031647038	25.56546620887225	24.893748997499777
31	25.492484511099235	24.50025265682249	24.458470341005388	25.548792491072884
32	25.423181282325373	24.437001483980747	24.310868728835327	25.828948504858552
33	24.91273389671193	24.1937419660223	25.06926326321386	25.82426087405191
34	25.60112257780116	24.436089171352954	25.18301618753151	24.779772063314375
35	26.150723590787035	24.112196391355944	25.199825916462338	24.537254101394687
36	24.674994728128464	24.945430521219844	24.71789878903989	25.661675961611802
37	25.92526745278389	24.64828031014708	24.70879544594159	24.71765679112744
38	25.11969625165797	24.158985561297886	25.10870388182081	25.612614305223342
39	25.921052852963	24.25491057132169	24.307629493657185	25.516407082058123
40	25.88282470254703	24.435028644657624	24.999397102164544	24.6827495506308
41	24.730346959561018	25.05731809636619	25.35736879652188	24.854966147550915
42	26.233439766769994	25.00413637763249	24.655671099928714	24.1067527556688
43	25.604995658972467	23.895505746533345	25.069546177540175	25.42995241695401
44	25.0241870487538	24.68335297274954	24.648187107083146	25.644272871413516
45	25.217861441357165	24.9404659750288	24.5976195363581	25.24405304725594
46	24.871355252435347	24.754081719069596	24.818859782693433	25.555703245801624
47	25.252981289788153	24.52848707009445	25.129820637485366	25.088711002632024
48	26.266374945807108	25.065578303326504	23.911557204976155	24.756489545890233
49	25.17668265658029	25.309043919822727	24.23164567114171	25.282627752455273
50	25.525346997103775	25.245932164225476	24.137526836611308	25.091194002059435
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	88.0
1	73.5
2	59.0
3	139.5
4	220.0
5	243.5
6	267.0
7	222.0
8	177.0
9	247.5
10	318.0
11	533.0
12	748.0
13	1417.5
14	2087.0
15	2592.5
16	3098.0
17	3045.0
18	2992.0
19	2975.5
20	2959.0
21	3641.0
22	4323.0
23	5150.5
24	5978.0
25	7866.0
26	9754.0
27	12401.0
28	15048.0
29	17611.0
30	20174.0
31	22975.0
32	25776.0
33	29487.0
34	33198.0
35	38808.5
36	44419.0
37	53513.5
38	62608.0
39	68606.5
40	74605.0
41	81784.0
42	88963.0
43	91538.0
44	94113.0
45	103603.5
46	113094.0
47	121531.5
48	129969.0
49	136096.5
50	142224.0
51	136612.5
52	131001.0
53	130950.5
54	130900.0
55	133024.0
56	135148.0
57	131980.0
58	128812.0
59	117660.5
60	106509.0
61	93739.5
62	80970.0
63	69977.5
64	58985.0
65	49784.5
66	40584.0
67	35276.5
68	29969.0
69	27035.5
70	24102.0
71	18824.0
72	13546.0
73	11927.0
74	10308.0
75	7536.5
76	4765.0
77	4292.0
78	3819.0
79	3275.0
80	2731.0
81	2127.0
82	1523.0
83	1301.5
84	1080.0
85	838.5
86	597.0
87	417.5
88	238.0
89	159.5
90	81.0
91	59.5
92	38.0
93	29.0
94	20.0
95	26.5
96	33.0
97	28.5
98	24.0
99	18.5
100	13.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03224802375697889
2	0.0045427650857657225
3	5.047516761961914E-4
4	5.6083519577354595E-5
5	0.0
6	2.2433407830941838E-4
7	0.0
8	0.0
9	0.0
10	1.1216703915470919E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	4.4866815661883676E-4
17	0.0
18	5.6083519577354595E-5
19	0.0
20	2.80417597886773E-4
21	0.0
22	0.0
23	0.0
24	0.0010095033523923828
25	1.1216703915470919E-4
26	2.2433407830941838E-4
27	3.925846370414822E-4
28	0.001121670391547092
29	4.4866815661883676E-4
30	7.290857545056098E-4
31	2.2433407830941838E-4
32	7.290857545056098E-4
33	1.682505587320638E-4
34	8.973363132376735E-4
35	1.682505587320638E-4
36	3.925846370414822E-4
37	0.001682505587320638
38	0.0
39	7.851692740829644E-4
40	0.0
41	0.0012899209502791557
42	0.005271850840271332
43	0.003757595811682758
44	0.004262347487878949
45	0.0022994243026715384
46	0.002467674861403602
47	0.0010095033523923828
48	0.004038013409569531
49	0.0031406770963318576
50	0.0024115913418262477
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	1783055.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.54718805170053
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.25832139515582	38.54643712067959
2	13.875444020277497	14.027293563876206
3	4.294314434734437	6.511965578569609
4	1.7925624512055478	3.6243596526201634
5	0.9194197725838089	2.323704207162276
6	0.5935628493821833	1.8001759780934645
7	0.396793276608538	1.4039749059267537
8	0.2920673759126309	1.1810547659217971
9	0.21664777233810165	0.9855842120420425
>10	1.1868013003018467	10.803951665111404
>50	0.0850722189197588	3.014054928422255
>100	0.07235634646432078	7.731878807622326
>500	0.010942315700223918	3.7681572569536628
>1k	0.005694470415422651	4.2774073569984905
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	2741	0.15372492716152897	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	2556	0.14334947603971837	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	2389	0.13398352827030013	No Hit
ATCAGATACCGTCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCG	2342	0.13134760285016447	No Hit
TCGTAGTTCCGACCATAAACGATGCCGACTGGCGATGCGGCGGCGTTATT	2300	0.12899209502791556	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTGGATACCGCAGCTAGGAATAATGGAA	2275	0.1275900070384817	No Hit
GGGTAGGCACACGCTGAGCCAGTCAGTGTAGCGCGCGTGCAGCCCCGGAC	2197	0.12321549251144805	No Hit
GAACTACGACGGTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGA	2017	0.11312045898752422	No Hit
GTCTTGCGCCGGTCCAAGAATTTCACCTCTAGCGGCGCAATACGAATGCC	1946	0.10913852909753205	No Hit
GTATCTGATCGTCTTCGAACCTCCGACTTTCGTTCTTGATTAATGAAAAC	1940	0.10880202798006793	No Hit
GTGTAGCGCGCGTGCAGCCCCGGACATCTAAGGGCATCACAGACCTGTTA	1885	0.10571743440331341	No Hit
GAATAACGCCGCCGCATCGCCAGTCGGCATCGTTTATGGTCGGAACTACG	1805	0.10123075283712504	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	5.6083519577354595E-5
2	0.0	0.0	0.0	0.0	5.6083519577354595E-5
3	0.0	0.0	0.0	0.0	5.6083519577354595E-5
4	0.0	0.0	0.0	0.0	5.6083519577354595E-5
5	0.0	0.0	0.0	0.0	5.6083519577354595E-5
6	0.0	0.0	0.0	0.0	1.1216703915470919E-4
7	0.0	0.0	0.0	0.0	1.1216703915470919E-4
8	0.0	0.0	0.0	0.0	1.1216703915470919E-4
9	0.0	0.0	0.0	0.0	1.1216703915470919E-4
10	0.0	0.0	0.0	0.0	1.1216703915470919E-4
11	0.0	0.0	0.0	0.0	1.1216703915470919E-4
12	0.0	0.0	0.0	0.0	3.925846370414822E-4
13	0.0	0.0	0.0	0.0	5.047516761961914E-4
14	0.0	0.0	0.0	0.0	5.047516761961914E-4
15	0.0	0.0	0.0	0.0	6.169187153509005E-4
16	5.6083519577354595E-5	0.0	0.0	0.0	6.169187153509005E-4
17	5.6083519577354595E-5	0.0	0.0	0.0	6.169187153509005E-4
18	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	6.730022349282551E-4
19	5.6083519577354595E-5	0.0	0.0	5.6083519577354595E-5	6.730022349282551E-4
20	5.6083519577354595E-5	0.0	0.0	1.1216703915470919E-4	8.412527936603189E-4
21	5.6083519577354595E-5	0.0	0.0	1.1216703915470919E-4	8.973363132376735E-4
22	5.6083519577354595E-5	0.0	0.0	1.6825055873206379E-4	8.973363132376735E-4
23	5.6083519577354595E-5	0.0	0.0	2.2433407830941838E-4	8.973363132376735E-4
24	5.6083519577354595E-5	0.0	0.0	4.4866815661883676E-4	8.973363132376735E-4
25	5.6083519577354595E-5	0.0	0.0	5.60835195773546E-4	8.973363132376735E-4
26	5.6083519577354595E-5	0.0	0.0	6.169187153509005E-4	0.0010095033523923828
27	5.6083519577354595E-5	0.0	0.0	0.0010095033523923828	0.0010095033523923828
28	5.6083519577354595E-5	0.0	0.0	0.0013460044698565103	0.0010095033523923828
29	5.6083519577354595E-5	0.0	0.0	0.00207509022436212	0.0010095033523923828
30	5.6083519577354595E-5	0.0	0.0	0.0031406770963318576	0.0010095033523923828
31	5.6083519577354595E-5	0.0	0.0	0.0063374377122410695	0.0010655868719697374
32	5.6083519577354595E-5	0.0	0.0	0.008805112573644672	0.0011777539111244464
33	5.6083519577354595E-5	0.0	0.0	0.011777539111244465	0.0011777539111244464
34	5.6083519577354595E-5	0.0	0.0	0.015927719559968707	0.0012899209502791557
35	5.6083519577354595E-5	0.0	0.0	0.019965732969538238	0.0012899209502791557
36	5.6083519577354595E-5	0.0	0.0	0.026022753083892534	0.0012899209502791557
37	5.6083519577354595E-5	0.0	0.0	0.03320144358979392	0.0012899209502791557
38	5.6083519577354595E-5	0.0	0.0	0.04492289918146103	0.0014581715090112195
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTATCAA	2055	0.0	30.627373	1
GGTATCA	760	0.0	24.033669	1
CGTCGTA	755	0.0	19.522581	10
TCAACGC	3220	0.0	19.403131	4
ATACCGT	760	0.0	19.394144	6
ATCAACG	3225	0.0	19.37305	3
CAACGCA	3250	0.0	19.224028	5
CCGTCGT	750	0.0	19.066084	9
TACCGTC	780	0.0	18.896858	7
ACCGTCG	800	0.0	18.699429	8
GTCCTAA	295	0.0	18.649775	1
AACGCAG	3395	0.0	18.402971	6
GTCCTAT	530	0.0	18.269741	1
GTATATA	230	0.0	18.179476	1
TATCAAC	3525	0.0	18.036327	2
TACGACG	690	0.0	17.854527	5
GTCGTAG	805	0.0	17.763432	11
CGTCTTA	140	8.3815394E-8	17.285185	15
CGACGGT	695	0.0	17.093002	7
ATTACAC	335	0.0	17.074104	3
>>END_MODULE