FastQCFastQC Report
Thu 2 Feb 2017
SRR4062098_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062098_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769357
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT13560.17625107719823177No Hit
TATCAACGCAGAGTACTTTTTTTTT9140.11880050483715622No Hit
GCGCAAGACGGACCAGAGCGAAAGC8880.11542105940415177No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG7870.10229321368363453No Hit
GAATAGGACCGCGGTTCTATTTTGT7780.10112340564913298No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGACG359.928713E-519.0006513
CGGTCCA1200.015.83593610
TCGACGC456.7550753E-414.77828314
ACGCTTA456.758409E-414.77732217
CGCTTAC456.758409E-414.77732218
GTCTTAG851.7185812E-814.5251851
CGAACGA400.005276969614.24956116
TGACTCG759.65776E-713.93290417
CGACCAT1301.8189894E-1213.88689810
TCGGCGT551.9575543E-413.81865613
TAGGACT551.9597166E-413.8168574
TAAATCC903.633795E-813.7209064
GTATAGG707.2729617E-613.5674791
CCGTCGT1550.013.4860889
CCGACCA1550.013.4860889
ATAAACG1500.013.29959115
GACGCTT500.001498693513.2995916
GTATCAA8500.013.0726651
CGTCGTA1600.013.06464710
GACCATA1403.6379788E-1212.89497611
Produced by FastQC (version 0.11.5)