##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062098_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	769357
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.187365553312702	32.0	32.0	32.0	32.0	32.0
2	30.67420066367109	32.0	32.0	32.0	32.0	32.0
3	30.71453174534059	32.0	32.0	32.0	32.0	32.0
4	30.720154622626428	32.0	32.0	32.0	32.0	32.0
5	30.63274006735495	32.0	32.0	32.0	32.0	32.0
6	34.27454874655069	36.0	36.0	36.0	32.0	36.0
7	34.20658549931956	36.0	36.0	36.0	32.0	36.0
8	34.15478509976513	36.0	36.0	36.0	32.0	36.0
9	34.291345890139425	36.0	36.0	36.0	32.0	36.0
10	33.960056254768595	36.0	36.0	36.0	32.0	36.0
11	34.2942496136384	36.0	36.0	36.0	32.0	36.0
12	34.08657229348664	36.0	36.0	36.0	32.0	36.0
13	34.1935226429343	36.0	36.0	36.0	32.0	36.0
14	34.088901511262	36.0	36.0	36.0	32.0	36.0
15	34.03830991334322	36.0	36.0	36.0	32.0	36.0
16	34.0390845862194	36.0	36.0	36.0	32.0	36.0
17	33.970438950968145	36.0	36.0	36.0	32.0	36.0
18	33.98010806426665	36.0	36.0	36.0	32.0	36.0
19	33.98520842729708	36.0	36.0	36.0	32.0	36.0
20	33.978450836217775	36.0	36.0	36.0	32.0	36.0
21	33.943820619036416	36.0	36.0	36.0	32.0	36.0
22	33.917735199653734	36.0	36.0	36.0	32.0	36.0
23	33.88403173039304	36.0	36.0	36.0	32.0	36.0
24	33.85594203991125	36.0	36.0	36.0	32.0	36.0
25	33.509916722665814	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	11.0
5	59.0
6	162.0
7	40.0
8	128.0
9	114.0
10	52.0
11	10.0
12	33.0
13	27.0
14	89.0
15	162.0
16	229.0
17	328.0
18	481.0
19	716.0
20	1117.0
21	1527.0
22	2397.0
23	3630.0
24	5009.0
25	7159.0
26	9782.0
27	12773.0
28	16672.0
29	22217.0
30	29156.0
31	39093.0
32	54595.0
33	76556.0
34	160955.0
35	324077.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.198992914789	17.39158691309146	11.5212991763166	25.888120995802943
2	17.107707105302495	19.39039845065022	36.832151607515385	26.669742836531903
3	19.381870220821913	22.55245641467571	28.152486569884044	29.913186794618333
4	13.086019506576937	15.38310547928645	34.859654299675405	36.67122071446121
5	15.356980794509967	36.002329987010846	32.82148164810175	15.819207570377438
6	35.02564742849414	34.46288666229672	16.43958547928957	14.071880429919572
7	31.15547552382043	29.96276269087048	19.728227493676684	19.153534291632404
8	28.551149670556153	32.50434574429328	18.909322272069616	20.035182313080952
9	27.051880961365793	14.64430246327213	18.64526539057112	39.65855118479095
10	16.887218984504944	26.14651198504895	30.28889577211098	26.677373258335134
11	37.12762592943032	21.411679879012997	21.648161572831025	19.812532618725655
12	24.64343767854145	23.60033264834151	28.01143706426126	23.744792608855786
13	29.418340443141922	19.231359851748127	25.134496409524974	26.215803295584976
14	23.825528681250617	19.592825653597323	24.530523762680282	32.05112190247178
15	25.572963166374706	26.801921980702105	21.70661682982506	25.918498023098124
16	26.035634627947108	25.843043153503213	23.167843469977853	24.95347874857183
17	24.20636469874783	25.803655784681933	24.827764807541648	25.162214709028586
18	24.965030688417254	25.178554931421115	25.505152036807882	24.35126234335375
19	25.74593757724783	24.948154508671273	25.03063892900355	24.275268985077343
20	25.981497358345244	24.11549124630682	24.552232689387978	25.350778705959954
21	26.932799073718723	24.111934327698023	24.163842506163284	24.791424092419977
22	26.211501604926546	23.96850778913074	24.692839048051514	25.127151557891203
23	24.721103514303852	24.189977417709926	25.23740022686356	25.85151884112266
24	24.9145252443251	24.809145408561527	24.927144804904195	25.34918454220918
25	25.18450484532555	24.395098604228814	24.915471867970894	25.504924682474737
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	391.0
1	391.0
2	316.0
3	241.0
4	241.0
5	241.0
6	573.0
7	905.0
8	905.0
9	905.0
10	1012.5
11	1120.0
12	1120.0
13	1120.0
14	1528.5
15	1937.0
16	1937.0
17	1937.0
18	3256.0
19	4575.0
20	4575.0
21	4575.0
22	7351.0
23	10127.0
24	10127.0
25	10127.0
26	14776.5
27	19426.0
28	19426.0
29	19426.0
30	24500.5
31	29575.0
32	29575.0
33	29575.0
34	36962.0
35	44349.0
36	44349.0
37	44349.0
38	52439.5
39	60530.0
40	60530.0
41	60530.0
42	69863.0
43	79196.0
44	79196.0
45	79196.0
46	88470.5
47	97745.0
48	97745.0
49	97745.0
50	101501.0
51	105257.0
52	105257.0
53	105257.0
54	98435.0
55	91613.0
56	91613.0
57	91613.0
58	84977.5
59	78342.0
60	78342.0
61	78342.0
62	69474.0
63	60606.0
64	60606.0
65	60606.0
66	50102.0
67	39598.0
68	39598.0
69	39598.0
70	30389.5
71	21181.0
72	21181.0
73	21181.0
74	16177.0
75	11173.0
76	11173.0
77	11173.0
78	8855.0
79	6537.0
80	6537.0
81	6537.0
82	4618.5
83	2700.0
84	2700.0
85	2700.0
86	2029.0
87	1358.0
88	1358.0
89	1358.0
90	905.0
91	452.0
92	452.0
93	452.0
94	279.5
95	107.0
96	107.0
97	107.0
98	211.5
99	316.0
100	316.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010398293640013674
2	2.5995734100034185E-4
3	5.199146820006837E-4
4	0.010138336299013331
5	0.03301458230704341
6	0.06095999646458017
7	0.10047351229663212
8	0.10255317102463486
9	0.11269150732364817
10	0.127769033101668
11	0.13127845720517262
12	0.12711913974916716
13	0.1215300569176598
14	0.10931206189064376
15	0.127769033101668
16	0.11581099541565229
17	0.12101014223565912
18	0.10736238183314119
19	0.09449449345362426
20	0.09228485605512135
21	0.08994523998611828
22	0.10216323501313433
23	0.07967692501660477
24	0.09215487738462118
25	0.08812553859911589
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	769357.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	60.04729073848672
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.95022334375278	49.20888887206245
2	11.287906848165932	13.5561644868155
3	2.9714495635802063	5.352824875771503
4	1.2086267537825797	2.902990483147839
5	0.6165000127357452	1.8509577752512032
6	0.3879369635322069	1.397673818245449
7	0.280225736138702	1.1778757375228974
8	0.18542939943902084	0.8907626447662296
9	0.14823284000056908	0.8010882396455181
>10	0.7998348295126035	9.011586812979921
>50	0.08931443713491938	3.7759462239399326
>100	0.06997311595561742	8.220318175327673
>500	0.004128848455681732	1.6759808661622764
>1k	2.1730781345693324E-4	0.17694098836157326
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1356	0.17625107719823177	No Hit
TATCAACGCAGAGTACTTTTTTTTT	914	0.11880050483715622	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	888	0.11542105940415177	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	787	0.10229321368363453	No Hit
GAATAGGACCGCGGTTCTATTTTGT	778	0.10112340564913298	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	1.2997867050017092E-4
6	0.0	0.0	0.0	0.0	2.5995734100034185E-4
7	0.0	0.0	0.0	0.0	2.5995734100034185E-4
8	0.0	0.0	0.0	0.0	2.5995734100034185E-4
9	0.0	0.0	0.0	0.0	2.5995734100034185E-4
10	0.0	0.0	0.0	0.0	2.5995734100034185E-4
11	0.0	0.0	0.0	0.0	2.5995734100034185E-4
12	0.0	0.0	0.0	0.0	6.498933525008546E-4
13	0.0	0.0	0.0	0.0	6.498933525008546E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCGACG	35	9.928713E-5	19.00065	13
CGGTCCA	120	0.0	15.835936	10
TCGACGC	45	6.7550753E-4	14.778283	14
ACGCTTA	45	6.758409E-4	14.777322	17
CGCTTAC	45	6.758409E-4	14.777322	18
GTCTTAG	85	1.7185812E-8	14.525185	1
CGAACGA	40	0.0052769696	14.249561	16
TGACTCG	75	9.65776E-7	13.932904	17
CGACCAT	130	1.8189894E-12	13.886898	10
TCGGCGT	55	1.9575543E-4	13.818656	13
TAGGACT	55	1.9597166E-4	13.816857	4
TAAATCC	90	3.633795E-8	13.720906	4
GTATAGG	70	7.2729617E-6	13.567479	1
CCGTCGT	155	0.0	13.486088	9
CCGACCA	155	0.0	13.486088	9
ATAAACG	150	0.0	13.299591	15
GACGCTT	50	0.0014986935	13.29959	16
GTATCAA	850	0.0	13.072665	1
CGTCGTA	160	0.0	13.064647	10
GACCATA	140	3.6379788E-12	12.894976	11
>>END_MODULE