FastQCFastQC Report
Thu 2 Feb 2017
SRR4062098_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062098_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences769357
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT9100.11828059015515553No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA8810.11451120871065058No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT8290.10775231784464169No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACCGC502.3609045E-630.7984526
GTATCAA8950.024.5861631
GGTATCA4550.024.1808991
CACACCG551.5934266E-423.9987965
ATACCGT2150.023.53376
TACCGTC2200.022.9988467
ACCGTCG2250.022.487768
AGTTCGC602.8711758E-422.00032632
TAATACC703.214641E-521.9988944
TGGTCGA500.002580345621.99889410
TATACTT1158.763891E-921.0424215
CCGTCGT2350.020.594719
CGTCGTA2500.020.23898510
CTATTCC1705.456968E-1219.4107914
TAGTCGA808.9821406E-519.25028436
GGACCGT808.986625E-519.2490356
CGGTCCA1950.019.17852410
GTATAAT1051.7873681E-618.86111
AGTCGAT708.118852E-418.8574237
CTATACT1051.791821E-618.8561954
Produced by FastQC (version 0.11.5)