##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062098_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	769357
Sequences flagged as poor quality	0
Sequence length	50
%GC	50
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.268619119602473	32.0	32.0	32.0	32.0	32.0
2	31.42990315289261	32.0	32.0	32.0	32.0	32.0
3	31.557061286242927	32.0	32.0	32.0	32.0	32.0
4	31.63178212455336	32.0	32.0	32.0	32.0	32.0
5	31.605744797278767	32.0	32.0	32.0	32.0	32.0
6	35.24036435620915	36.0	36.0	36.0	36.0	36.0
7	35.23973395965722	36.0	36.0	36.0	36.0	36.0
8	35.1946144637665	36.0	36.0	36.0	36.0	36.0
9	35.278882235425165	36.0	36.0	36.0	36.0	36.0
10	35.15444715522183	36.0	36.0	36.0	36.0	36.0
11	35.27887833606505	36.0	36.0	36.0	36.0	36.0
12	35.19022898342382	36.0	36.0	36.0	36.0	36.0
13	35.23054316786615	36.0	36.0	36.0	36.0	36.0
14	35.191909607633384	36.0	36.0	36.0	36.0	36.0
15	35.17571556507577	36.0	36.0	36.0	36.0	36.0
16	35.18948290585515	36.0	36.0	36.0	36.0	36.0
17	35.16573190339465	36.0	36.0	36.0	36.0	36.0
18	35.161813046479075	36.0	36.0	36.0	36.0	36.0
19	35.14819258159736	36.0	36.0	36.0	36.0	36.0
20	35.14769736286276	36.0	36.0	36.0	36.0	36.0
21	35.13371815685046	36.0	36.0	36.0	36.0	36.0
22	35.12420761752996	36.0	36.0	36.0	36.0	36.0
23	35.09141269917607	36.0	36.0	36.0	36.0	36.0
24	35.07621559302118	36.0	36.0	36.0	36.0	36.0
25	35.044602180782135	36.0	36.0	36.0	36.0	36.0
26	35.02478693246438	36.0	36.0	36.0	36.0	36.0
27	35.00449336263919	36.0	36.0	36.0	36.0	36.0
28	34.98129476952832	36.0	36.0	36.0	36.0	36.0
29	34.97204288776212	36.0	36.0	36.0	36.0	36.0
30	34.94987372572161	36.0	36.0	36.0	32.0	36.0
31	34.95505467552775	36.0	36.0	36.0	36.0	36.0
32	34.922203866345534	36.0	36.0	36.0	32.0	36.0
33	34.90097055073262	36.0	36.0	36.0	32.0	36.0
34	34.879647549837074	36.0	36.0	36.0	32.0	36.0
35	34.86862405879195	36.0	36.0	36.0	32.0	36.0
36	34.843266519964075	36.0	36.0	36.0	32.0	36.0
37	34.83594872081491	36.0	36.0	36.0	32.0	36.0
38	34.813524800580225	36.0	36.0	36.0	32.0	36.0
39	34.80555840786527	36.0	36.0	36.0	32.0	36.0
40	34.80126261280524	36.0	36.0	36.0	32.0	36.0
41	34.76483219103745	36.0	36.0	36.0	32.0	36.0
42	34.73747558025728	36.0	36.0	36.0	32.0	36.0
43	34.741939047802255	36.0	36.0	36.0	32.0	36.0
44	34.680019808749385	36.0	36.0	36.0	32.0	36.0
45	34.66319926899996	36.0	36.0	36.0	32.0	36.0
46	34.65389539576555	36.0	36.0	36.0	32.0	36.0
47	34.624620299808804	36.0	36.0	36.0	32.0	36.0
48	34.60740072554094	36.0	36.0	36.0	32.0	36.0
49	34.58737231220357	36.0	36.0	36.0	32.0	36.0
50	34.17485640606377	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	1.0
20	3.0
21	15.0
22	63.0
23	126.0
24	362.0
25	829.0
26	1753.0
27	3559.0
28	6238.0
29	9958.0
30	14815.0
31	21920.0
32	33622.0
33	57004.0
34	129206.0
35	489883.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.806226214174174	18.02567406446211	12.066264206484698	26.101835514879024
2	16.53115791416609	19.884365864532842	36.64806589689896	26.936410324402104
3	18.71349209856581	22.97498940669705	28.4309953545442	29.880523140192945
4	12.733100498208245	15.767452561034734	35.200693566185784	36.298753374571234
5	14.96808373745868	36.29303431306923	32.71576134356352	16.02312060590857
6	34.97106671033956	34.865393913870825	16.289468074597455	13.874071301192165
7	31.14028987843095	30.053408235708517	19.77911424735201	19.027187638508522
8	28.479236557280952	32.70380330587751	18.832349611428764	19.98461052541278
9	27.25496745983984	14.271528042248265	18.643230645851016	39.83027385206088
10	16.81653226854668	26.187876613687294	30.465870156338546	26.52972096142748
11	37.425018554455214	21.224607041984413	21.72645468878557	19.623919714774807
12	24.70673562468399	23.395510796678266	28.081891761561927	23.815861817075817
13	29.442508484357717	19.15833611704319	25.131245962537545	26.267909436061544
14	23.913605777291945	19.37800007018848	24.65552402850692	32.05287012401265
15	25.632833651997704	26.817719212277265	21.812890504668182	25.736556631056846
16	26.17865424409113	25.75089165947447	23.198353939419146	24.872100157015254
17	24.364371806586536	25.806485155785936	24.719603513063507	25.109539524564017
18	25.074965198210975	24.911452030721758	25.69418358447379	24.31939918659348
19	25.87719355253803	24.838274039230164	25.162180886116587	24.12235152211522
20	26.06413675853571	24.10717535185272	24.54338433702993	25.285303552581638
21	27.014766876755523	23.94675033826949	24.24362162169188	24.794861163283105
22	26.331209048595127	23.966507096185516	24.769645301206072	24.932638554013288
23	24.688408632143467	24.014729182941082	25.402641426541905	25.89422075837355
24	24.933742511506512	24.674691654091067	25.03239760472193	25.359168229680495
25	25.110254693866942	24.2336762612838	25.116753644286447	25.539315400562806
26	24.824690812433793	25.10804505072431	25.23581441597182	24.83144972087008
27	25.48495357131716	24.438228535182855	24.75628840894675	25.320529484553234
28	24.96201294354519	24.427399553643962	25.40394961402031	25.206637888790535
29	24.764737077436596	24.64502594391124	25.181841341804535	25.40839563684763
30	24.858093204201506	24.75787782562524	25.512937662395057	24.871091307778194
31	25.351658664281985	24.58153203856742	24.57542301723264	25.491386279917954
32	25.320758199464745	24.52878992498853	24.523590724105702	25.626861151441023
33	25.094787191868512	24.188183608347252	24.955319715865887	25.76170948391835
34	25.42597591996371	24.491292011058672	25.170435860874946	24.912296208102674
35	26.07427510801242	24.114194209182745	25.11321156915654	24.6983191136483
36	24.81672983638264	24.758759273990194	24.90238589157685	25.522124998050316
37	25.84426371209571	24.737859383184784	24.68859631449855	24.729280590220956
38	25.182067622703112	24.32030903728698	25.085623449191985	25.411999890817917
39	25.798631826730983	24.36793800456751	24.557969290840155	25.275460877861356
40	25.936237580521894	24.449149678432352	24.943849141359784	24.670763599685973
41	24.72041260911837	24.9766035656166	25.413207027249047	24.889776798015983
42	26.113496362329407	24.85509761331893	24.791014297208804	24.24039172714286
43	25.628540455102023	23.958368537491452	24.941961883320637	25.471129124085884
44	25.03139160342851	24.780258776000437	24.735933571987513	25.452416048583544
45	25.22938462918264	25.035452419668264	24.68151313916716	25.05364981198194
46	25.213625220644243	24.815620702474074	24.600500690203788	25.3702533866779
47	25.080002599597062	24.571651394033925	25.169428738545523	25.178917267823486
48	26.044417985566625	25.087478942661644	24.07854647172597	24.789556600045753
49	25.185648310025204	25.132485237173597	24.439675510281013	25.24219094252019
50	25.250830251707622	25.19571843581228	24.400106584172253	25.153344728307847
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	270.0
1	157.0
2	44.0
3	86.5
4	129.0
5	123.0
6	117.0
7	99.0
8	81.0
9	125.5
10	170.0
11	251.0
12	332.0
13	623.5
14	915.0
15	1187.5
16	1460.0
17	1498.5
18	1537.0
19	1501.5
20	1466.0
21	1725.5
22	1985.0
23	2400.0
24	2815.0
25	3456.0
26	4097.0
27	5297.5
28	6498.0
29	7603.0
30	8708.0
31	9919.0
32	11130.0
33	12798.5
34	14467.0
35	16670.5
36	18874.0
37	22564.0
38	26254.0
39	29318.5
40	32383.0
41	35015.0
42	37647.0
43	39232.5
44	40818.0
45	44617.0
46	48416.0
47	52356.0
48	56296.0
49	58454.0
50	60612.0
51	59388.0
52	58164.0
53	57855.5
54	57547.0
55	57971.5
56	58396.0
57	56653.5
58	54911.0
59	49845.5
60	44780.0
61	39385.5
62	33991.0
63	29951.5
64	25912.0
65	21588.5
66	17265.0
67	15188.5
68	13112.0
69	11764.5
70	10417.0
71	8252.0
72	6087.0
73	5351.5
74	4616.0
75	3382.5
76	2149.0
77	1946.5
78	1744.0
79	1483.5
80	1223.0
81	953.5
82	684.0
83	571.0
84	458.0
85	351.5
86	245.0
87	160.0
88	75.0
89	49.0
90	23.0
91	18.0
92	13.0
93	9.0
94	5.0
95	7.5
96	10.0
97	7.5
98	5.0
99	4.5
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03301458230704341
2	0.004419274797005812
3	3.8993601150051274E-4
4	0.0
5	0.0
6	1.2997867050017092E-4
7	0.0
8	0.0
9	0.0
10	1.2997867050017092E-4
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	6.498933525008546E-4
17	0.0
18	0.0
19	0.0
20	1.2997867050017092E-4
21	0.0
22	0.0
23	0.0
24	0.0012997867050017092
25	2.5995734100034185E-4
26	2.5995734100034185E-4
27	6.498933525008546E-4
28	0.0020796587280027348
29	6.498933525008546E-4
30	0.001819701387002393
31	3.8993601150051274E-4
32	0.0010398293640013674
33	2.5995734100034185E-4
34	7.798720230010255E-4
35	1.2997867050017092E-4
36	1.2997867050017092E-4
37	0.0023396160690030763
38	0.0
39	0.0012997867050017092
40	1.2997867050017092E-4
41	0.0011698080345015382
42	0.005979018843007862
43	0.004549253467505982
44	0.0055890828315073495
45	0.0020796587280027348
46	0.0024695947395032476
47	9.098506935011965E-4
48	0.0037693814445049567
49	0.003379445433004444
50	0.001559744046002051
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	769357.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.6616960373282
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.8014145328713	50.44013958349392
2	11.599026601475265	14.304313052581033
3	2.943458344411174	5.444959011948572
4	1.1968156100280574	2.9519072143311837
5	0.6245664573876003	1.925591352527255
6	0.3641023332647838	1.3470700440153043
7	0.25714128737638564	1.1099037530594962
8	0.17977714224695307	0.8868290799752907
9	0.13886720766964072	0.7706508793990124
>10	0.7523007592717583	8.647628768713377
>50	0.07782094905131932	3.3642044222976066
>100	0.06111384300606498	7.327875420398092
>500	0.003594931939713898	1.4789274172599596
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTTCATTAAT	910	0.11828059015515553	No Hit
GATTAAGAGGGACGGCCGGGGGCATTCGTATTGCGCCGCTAGAGGTGAAA	881	0.11451120871065058	No Hit
GAATAGGACCGCGGTTCTATTTTGTTGGTTTTCGGAACTGAGGCCATGAT	829	0.10775231784464169	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.5995734100034185E-4
13	0.0	0.0	0.0	0.0	2.5995734100034185E-4
14	0.0	0.0	0.0	0.0	2.5995734100034185E-4
15	0.0	0.0	0.0	1.2997867050017092E-4	5.199146820006837E-4
16	0.0	0.0	0.0	1.2997867050017092E-4	6.498933525008546E-4
17	0.0	0.0	0.0	1.2997867050017092E-4	6.498933525008546E-4
18	0.0	0.0	0.0	1.2997867050017092E-4	6.498933525008546E-4
19	0.0	0.0	0.0	1.2997867050017092E-4	6.498933525008546E-4
20	0.0	0.0	0.0	3.8993601150051274E-4	9.098506935011965E-4
21	0.0	0.0	0.0	5.199146820006837E-4	9.098506935011965E-4
22	0.0	0.0	0.0	6.498933525008546E-4	9.098506935011965E-4
23	0.0	0.0	0.0	7.798720230010255E-4	9.098506935011965E-4
24	0.0	0.0	0.0	9.098506935011965E-4	9.098506935011965E-4
25	0.0	0.0	0.0	0.0011698080345015384	9.098506935011965E-4
26	0.0	0.0	0.0	0.001559744046002051	9.098506935011965E-4
27	0.0	0.0	0.0	0.001819701387002393	0.0010398293640013674
28	0.0	0.0	0.0	0.0025995734100034184	0.0010398293640013674
29	0.0	0.0	0.0	0.0038993601150051275	0.0010398293640013674
30	0.0	0.0	0.0	0.007928698900510426	0.0010398293640013674
31	0.0	0.0	0.0	0.013127845720517263	0.0010398293640013674
32	0.0	0.0	0.0	0.018586949881524442	0.0010398293640013674
33	0.0	0.0	0.0	0.024955904736032816	0.0010398293640013674
34	0.0	0.0	0.0	0.03249466762504273	0.0012997867050017092
35	0.0	0.0	0.0	0.041723153230554866	0.0012997867050017092
36	0.0	0.0	0.0	0.05342123357557025	0.0012997867050017092
37	0.0	0.0	0.0	0.07265807680959555	0.0012997867050017092
38	0.0	0.0	0.0	0.0969640881931275	0.0012997867050017092
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACACCGC	50	2.3609045E-6	30.798452	6
GTATCAA	895	0.0	24.586163	1
GGTATCA	455	0.0	24.180899	1
CACACCG	55	1.5934266E-4	23.998796	5
ATACCGT	215	0.0	23.5337	6
TACCGTC	220	0.0	22.998846	7
ACCGTCG	225	0.0	22.48776	8
AGTTCGC	60	2.8711758E-4	22.000326	32
TAATACC	70	3.214641E-5	21.998894	4
TGGTCGA	50	0.0025803456	21.998894	10
TATACTT	115	8.763891E-9	21.042421	5
CCGTCGT	235	0.0	20.59471	9
CGTCGTA	250	0.0	20.238985	10
CTATTCC	170	5.456968E-12	19.410791	4
TAGTCGA	80	8.9821406E-5	19.250284	36
GGACCGT	80	8.986625E-5	19.249035	6
CGGTCCA	195	0.0	19.178524	10
GTATAAT	105	1.7873681E-6	18.8611	1
AGTCGAT	70	8.118852E-4	18.85742	37
CTATACT	105	1.791821E-6	18.856195	4
>>END_MODULE