FastQCFastQC Report
Thu 2 Feb 2017
SRR4062097_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062097_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3099752
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47420.15297998033391058No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA9500.014.7963891
GTATCAA29450.013.57733251
CTAGCGC604.096913E-412.6656233
CAAGACG5700.011.6668364
CGTCTTA907.4713134E-611.61052915
GTGTACG1900.011.4971941
CGTCGTA4050.011.49566510
ACCGTCG4150.011.4476138
CGCCGGT3750.011.4018227
TAGGACG2000.011.4001654
GCGTTAT1751.8189894E-1211.3972191
AAGACGG5750.011.2353085
CGACCAT4000.011.16428410
ACGGTAT2900.011.1397129
CCGTCGT4100.011.1237299
TACCGTC4600.010.9474027
CGCAAGA5700.010.8319162
GACTGCG1252.237557E-710.6417027
CGTTATT1803.6379788E-1110.5541732
ATAACGA1451.4437319E-810.48341812
Produced by FastQC (version 0.11.5)