##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062097_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3099752
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.220231489486903	32.0	32.0	32.0	32.0	32.0
2	30.776281779961753	32.0	32.0	32.0	32.0	32.0
3	30.804932781719312	32.0	32.0	32.0	32.0	32.0
4	30.807291357502148	32.0	32.0	32.0	32.0	32.0
5	30.731728860889515	32.0	32.0	32.0	32.0	32.0
6	34.350866456413286	36.0	36.0	36.0	32.0	36.0
7	34.29204820256588	36.0	36.0	36.0	32.0	36.0
8	34.25797644456718	36.0	36.0	36.0	32.0	36.0
9	34.38727517556243	36.0	36.0	36.0	32.0	36.0
10	34.09876854664502	36.0	36.0	36.0	32.0	36.0
11	34.35635108873226	36.0	36.0	36.0	32.0	36.0
12	34.20125642309449	36.0	36.0	36.0	32.0	36.0
13	34.2757381880873	36.0	36.0	36.0	32.0	36.0
14	34.19369420521384	36.0	36.0	36.0	32.0	36.0
15	34.14629621982662	36.0	36.0	36.0	32.0	36.0
16	34.14722855247775	36.0	36.0	36.0	32.0	36.0
17	34.0924025534946	36.0	36.0	36.0	32.0	36.0
18	34.09643545677203	36.0	36.0	36.0	32.0	36.0
19	34.09900469456911	36.0	36.0	36.0	32.0	36.0
20	34.088555148928045	36.0	36.0	36.0	32.0	36.0
21	34.06861871530368	36.0	36.0	36.0	32.0	36.0
22	34.03440242961372	36.0	36.0	36.0	32.0	36.0
23	34.00435196106011	36.0	36.0	36.0	32.0	36.0
24	33.9802051906088	36.0	36.0	36.0	32.0	36.0
25	33.62970263427526	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	59.0
5	255.0
6	656.0
7	129.0
8	572.0
9	410.0
10	201.0
11	65.0
12	132.0
13	145.0
14	336.0
15	538.0
16	957.0
17	1316.0
18	1852.0
19	2570.0
20	3893.0
21	5659.0
22	8499.0
23	12683.0
24	18406.0
25	26164.0
26	35904.0
27	47100.0
28	62925.0
29	84519.0
30	110243.0
31	150601.0
32	213148.0
33	300954.0
34	650226.0
35	1358634.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.18206327734869	16.89785428937268	11.640474507527939	26.279607925750685
2	16.94985681070979	18.77887941032531	36.87721853069755	27.394045248267357
3	19.562851825338168	21.883249718442947	27.82164364797287	30.73225480824602
4	13.218221139435848	14.870567343279095	34.28470764283924	37.626503874445824
5	15.394304439436542	35.7524335527078	33.18683985556333	15.66642215229233
6	36.098572412236905	34.14831378845824	15.74975716190788	14.00335663739697
7	31.58253727472637	29.670349544519585	19.559288347988517	19.187824832765525
8	28.535672173905464	33.35428273750795	18.352162498478048	19.757882590108544
9	26.72278890306802	14.694814345406915	18.190729689199216	40.39166706232585
10	16.13985601043532	26.855504239671202	30.926260506305482	26.078379243588003
11	37.49561851964922	21.24038295349735	21.446203497753935	19.817795029099493
12	24.322977185074922	23.345670003285257	28.404932212922073	23.926420598717748
13	29.39754054673898	19.474935477306424	24.883148513636172	26.244375462318427
14	23.487868843987393	19.116034055087592	24.64129760735879	32.754799493566225
15	25.24536074215204	27.17064548078172	21.61629592014074	25.967697856925504
16	25.97058466340576	25.917323628132678	23.096620492029903	25.015471216431663
17	24.376849409271102	26.125957348394873	24.621429293576934	24.87576394875709
18	24.964102431604072	25.379370928621487	25.266430040919673	24.390096598854765
19	25.53968624556108	25.136263692971315	24.920586175261636	24.40346388620597
20	25.75824113372581	24.547289809435487	24.65597900354677	25.038490053291934
21	26.147408434004156	24.414606644338903	24.54848280146676	24.889502120190176
22	25.935393948749315	24.229287572785875	24.78641780310749	25.048900675357324
23	24.832881096320673	24.513075698147617	25.144711539082447	25.509331666449263
24	24.877224989303905	24.83489267579938	24.92982555276605	25.35805678213066
25	24.967952878299723	24.543284117995405	24.982644505880202	25.50611849782467
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	155.0
1	155.0
2	453.0
3	751.0
4	751.0
5	751.0
6	1716.0
7	2681.0
8	2681.0
9	2681.0
10	3371.0
11	4061.0
12	4061.0
13	4061.0
14	5596.5
15	7132.0
16	7132.0
17	7132.0
18	12310.0
19	17488.0
20	17488.0
21	17488.0
22	27273.0
23	37058.0
24	37058.0
25	37058.0
26	54468.0
27	71878.0
28	71878.0
29	71878.0
30	93438.5
31	114999.0
32	114999.0
33	114999.0
34	144500.0
35	174001.0
36	174001.0
37	174001.0
38	208724.5
39	243448.0
40	243448.0
41	243448.0
42	284262.5
43	325077.0
44	325077.0
45	325077.0
46	361323.0
47	397569.0
48	397569.0
49	397569.0
50	413763.5
51	429958.0
52	429958.0
53	429958.0
54	409664.5
55	389371.0
56	389371.0
57	389371.0
58	358675.0
59	327979.0
60	327979.0
61	327979.0
62	285945.0
63	243911.0
64	243911.0
65	243911.0
66	198903.0
67	153895.0
68	153895.0
69	153895.0
70	117400.0
71	80905.0
72	80905.0
73	80905.0
74	60737.5
75	40570.0
76	40570.0
77	40570.0
78	31315.5
79	22061.0
80	22061.0
81	22061.0
82	15240.0
83	8419.0
84	8419.0
85	8419.0
86	6065.0
87	3711.0
88	3711.0
89	3711.0
90	2429.0
91	1147.0
92	1147.0
93	1147.0
94	726.5
95	306.0
96	306.0
97	306.0
98	763.5
99	1221.0
100	1221.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.001064601297136029
2	2.903458083098261E-4
3	8.065161341939614E-4
4	0.011000880070405634
5	0.03526088538695999
6	0.06481163654382674
7	0.10810542262735857
8	0.10978297618648201
9	0.11784813752842163
10	0.132623513106855
11	0.13991441895996842
12	0.13236542794391293
13	0.12717146403970384
14	0.11588023816098837
15	0.1324622098800162
16	0.1187191749533511
17	0.12359053240388264
18	0.1110411413558246
19	0.0967496754579076
20	0.09187831800737607
21	0.09226544575178917
22	0.10223398517042655
23	0.0824582095599906
24	0.09120084445465315
25	0.08871677476133574
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	3099752.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.83231141667903
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.88797761327507	35.10456109926699
2	14.849099362654474	14.502316886687069
3	5.432851303197742	7.95896060154788
4	2.5269795731734956	4.935930138431792
5	1.350320630594825	3.2969638772786456
6	0.851615041561202	2.4951798549986806
7	0.5798431417297025	1.9820556608839277
8	0.40160880218561357	1.5689188876805864
9	0.32316521221410627	1.4202813853688763
>10	1.6319520047636698	14.256801327860785
>50	0.10224802210156408	3.408426722856086
>100	0.05430260147039135	5.236111068599262
>500	0.00557919050085035	1.9122703660837812
>1k	0.0024575005772546014	1.9212221224557051
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	4742	0.15297998033391058	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	3.2260645367758453E-5
2	0.0	0.0	0.0	0.0	3.2260645367758453E-5
3	0.0	0.0	0.0	0.0	3.2260645367758453E-5
4	0.0	0.0	0.0	0.0	6.452129073551691E-5
5	0.0	0.0	0.0	0.0	9.678193610327536E-5
6	0.0	0.0	0.0	0.0	1.6130322683879225E-4
7	0.0	0.0	0.0	0.0	1.9356387220655072E-4
8	0.0	0.0	0.0	0.0	2.2582451757430916E-4
9	0.0	0.0	0.0	3.2260645367758453E-5	2.5808516294206763E-4
10	0.0	0.0	0.0	3.2260645367758453E-5	2.5808516294206763E-4
11	0.0	0.0	0.0	3.2260645367758453E-5	2.5808516294206763E-4
12	0.0	0.0	0.0	3.2260645367758453E-5	4.516490351486183E-4
13	0.0	3.2260645367758453E-5	0.0	3.2260645367758453E-5	4.516490351486183E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	950	0.0	14.796389	1
GTATCAA	2945	0.0	13.5773325	1
CTAGCGC	60	4.096913E-4	12.665623	3
CAAGACG	570	0.0	11.666836	4
CGTCTTA	90	7.4713134E-6	11.610529	15
GTGTACG	190	0.0	11.497194	1
CGTCGTA	405	0.0	11.495665	10
ACCGTCG	415	0.0	11.447613	8
CGCCGGT	375	0.0	11.401822	7
TAGGACG	200	0.0	11.400165	4
GCGTTAT	175	1.8189894E-12	11.397219	1
AAGACGG	575	0.0	11.235308	5
CGACCAT	400	0.0	11.164284	10
ACGGTAT	290	0.0	11.139712	9
CCGTCGT	410	0.0	11.123729	9
TACCGTC	460	0.0	10.947402	7
CGCAAGA	570	0.0	10.831916	2
GACTGCG	125	2.237557E-7	10.641702	7
CGTTATT	180	3.6379788E-11	10.554173	2
ATAACGA	145	1.4437319E-8	10.483418	12
>>END_MODULE