FastQCFastQC Report
Thu 2 Feb 2017
SRR4062097_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062097_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3099752
Sequences flagged as poor quality0
Sequence length50
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA30250.027.7901841
GGTATCA13100.026.5423411
CGCAATA4450.020.764636
CTAGCGG4900.018.8570429
CAATACG5100.018.55041338
TCAACGC44650.018.4760484
CAACGCA45100.018.3404755
ATACGAA5050.018.29840540
ATCAACG45350.018.1908633
TATCAAC46150.018.1627062
TCTAGCG5250.018.0192428
TACGAAT4900.017.96082341
CGAATGC5150.017.94367443
AACGCAG47050.017.7206176
ACCGTCG6750.017.2731448
GCGCAAT5500.016.80017935
CGTATAC1053.7791604E-516.7610023
ATACCGT7000.016.6562466
GTATTAT3850.016.5764181
AATACGA5600.016.10835539
Produced by FastQC (version 0.11.5)