FastQCFastQC Report
Thu 2 Feb 2017
SRR4062096_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062096_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382167
Sequences flagged as poor quality0
Sequence length25
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG13690.35822035916235573No Hit
TCCATGTACTCTGCGTTGATACCAC10710.28024397710948357No Hit
GTCCTAAAGTGTGTATTTCTCATTT9660.2527690773928675No Hit
CTGTAGGACGTGGAATATGGCAAGA9140.23916246039035288No Hit
GTCCTACAGTGGACATTTCTAAATT8520.2229391862719701No Hit
GTATCAACGCAGAGTACTTTTTTTT8050.2106408978273896No Hit
CTTTAGGACGTGAAATATGGCGAGG7780.20357592361454546No Hit
GTCCTACAGTGTGCATTTCTCATTT5480.14339280994957704No Hit
TATCAACGCAGAGTACTTTTTTTTT5300.13868282714101426No Hit
GAGTACATGGAAGCAGTGGTATCAA5290.13842116142942745No Hit
GTACATGGGAAGCAGTGGTATCAAC5060.13240285006293062No Hit
CCCATGTACTCTGCGTTGATACCAC4520.11827290163724236No Hit
CTGAAGGACCTGGAATATGGCGAGA4390.1148712473866137No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4240.11094626171281141No Hit
GCTTCCATGTACTCTGCGTTGATAC3990.10440461892314093No Hit
GTCCTTCAGTGTGCATTTCTCATTT3860.10100296467251228No Hit
CTGTAGGACCTGGAATATGGCGAGA3850.10074129896092546No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTTAAG359.9382996E-518.9941021
CGCCACT402.7552858E-416.62419717
TCGCCAG456.7482685E-414.77706417
GTCTAAG456.761697E-414.7731911
CCGTCGT400.00526197214.2530499
CGAGCCG400.005271135414.24931215
CTACACC500.001496478213.2993584
GATATAC1102.730303E-912.9505241
TATACAC1251.0186341E-1012.9176833
TAGGACC1800.012.6660554
TGAGTTA853.9119623E-612.29674712
TAGAGTG550.003062263812.0903255
AATGCCG550.003062263812.09032518
GCCGTTT550.003062263812.09032515
ATGCCGG550.00306527212.08873919
TAGAAAT951.0335516E-611.9994214
CACGCTG657.9847535E-411.69480910
GTCTTAT658.0023415E-411.69174316
CGTTTCT658.0023415E-411.69174317
TACACTG1800.011.6105515
Produced by FastQC (version 0.11.5)