##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062096_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382167
Sequences flagged as poor quality	0
Sequence length	25
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.255278451566983	32.0	32.0	32.0	32.0	32.0
2	30.888480690378813	32.0	32.0	32.0	32.0	32.0
3	30.901676492214136	32.0	32.0	32.0	32.0	32.0
4	30.909738412788126	32.0	32.0	32.0	32.0	32.0
5	30.86260456815999	32.0	32.0	32.0	32.0	32.0
6	34.50548582164342	36.0	36.0	36.0	32.0	36.0
7	34.439708295064726	36.0	36.0	36.0	32.0	36.0
8	34.391533020904475	36.0	36.0	36.0	32.0	36.0
9	34.469218954017485	36.0	36.0	36.0	32.0	36.0
10	34.22888684789634	36.0	36.0	36.0	32.0	36.0
11	34.494113829817856	36.0	36.0	36.0	32.0	36.0
12	34.3211580277732	36.0	36.0	36.0	32.0	36.0
13	34.390918106482246	36.0	36.0	36.0	32.0	36.0
14	34.303880240837124	36.0	36.0	36.0	32.0	36.0
15	34.26244024209311	36.0	36.0	36.0	32.0	36.0
16	34.26072371502511	36.0	36.0	36.0	32.0	36.0
17	34.20428503769295	36.0	36.0	36.0	32.0	36.0
18	34.20552271650875	36.0	36.0	36.0	32.0	36.0
19	34.17861563138628	36.0	36.0	36.0	32.0	36.0
20	34.150193501793716	36.0	36.0	36.0	32.0	36.0
21	34.09935447068951	36.0	36.0	36.0	32.0	36.0
22	34.05180981089419	36.0	36.0	36.0	32.0	36.0
23	34.02847707939199	36.0	36.0	36.0	32.0	36.0
24	33.983486276941754	36.0	36.0	36.0	32.0	36.0
25	33.61873474161819	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	5.0
5	31.0
6	91.0
7	17.0
8	88.0
9	45.0
10	23.0
11	6.0
12	16.0
13	12.0
14	100.0
15	171.0
16	231.0
17	221.0
18	323.0
19	422.0
20	548.0
21	793.0
22	1106.0
23	1583.0
24	2203.0
25	2970.0
26	4048.0
27	5220.0
28	7036.0
29	9305.0
30	12404.0
31	16676.0
32	23847.0
33	34245.0
34	77664.0
35	180717.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.25781669078378	17.88451524611226	12.05663551939879	25.801032543705173
2	15.038569828659949	19.927570362462188	39.98047958468092	25.053380224196943
3	18.8533255182303	24.431529754085922	28.96516173599954	27.74998299168424
4	12.223231178673394	16.303485959077076	36.68434811963374	34.78893474261579
5	13.543021828300095	37.63948621432516	34.32558431733882	14.491907640035913
6	33.10256161840448	35.71864354410891	17.677602138740582	13.501192698746028
7	29.07023954526999	31.074613964087856	21.08365093709481	18.77149555354734
8	26.74329963613311	35.562028957412636	19.596681450123516	18.097989956330736
9	27.116321718627194	15.075713911448782	19.336651820801677	38.47131254912235
10	16.14526149742289	27.53852563615449	32.246239148492386	24.06997371793024
11	35.436586650455546	21.442440472603117	23.88170772290511	19.23926515403623
12	24.916675400901372	23.87223561471544	30.150403521643433	21.060685462739755
13	29.931407790901183	20.921147779792285	25.234230079963112	23.913214349343423
14	22.34055094903132	21.665422250167012	25.56372067434275	30.430306126458916
15	23.926645600465342	30.17628532649992	22.71874525093409	23.178323822100648
16	23.70028818443804	26.416295520041917	26.778883940267225	23.104532355252815
17	22.41034186421849	27.57341382129158	27.216562912657988	22.79968140183194
18	23.128490742002075	25.266422860496064	29.763913193825907	21.841173203675954
19	25.03686629909504	25.99080634388465	25.941302041725056	23.03102531529526
20	24.88540820870112	25.92131800204301	26.969538227821577	22.223735561434296
21	24.426185281321782	25.44795920238248	25.031496619843	25.094358896452736
22	24.464724464724465	27.53545365485664	25.170654275131888	22.829167605287008
23	22.470733048738968	27.279155645182414	26.435062724249008	23.815048581829608
24	23.972215237616293	26.474886849383957	26.79495641605901	22.75794149694074
25	23.824340569973835	26.33843800663686	26.621565345842267	23.215656077547035
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	340.0
1	340.0
2	303.5
3	267.0
4	267.0
5	267.0
6	573.5
7	880.0
8	880.0
9	880.0
10	910.5
11	941.0
12	941.0
13	941.0
14	1181.0
15	1421.0
16	1421.0
17	1421.0
18	2388.0
19	3355.0
20	3355.0
21	3355.0
22	5391.0
23	7427.0
24	7427.0
25	7427.0
26	11492.5
27	15558.0
28	15558.0
29	15558.0
30	19979.0
31	24400.0
32	24400.0
33	24400.0
34	27301.0
35	30202.0
36	30202.0
37	30202.0
38	32451.5
39	34701.0
40	34701.0
41	34701.0
42	38204.5
43	41708.0
44	41708.0
45	41708.0
46	47758.5
47	53809.0
48	53809.0
49	53809.0
50	50941.0
51	48073.0
52	48073.0
53	48073.0
54	42757.5
55	37442.0
56	37442.0
57	37442.0
58	33825.0
59	30208.0
60	30208.0
61	30208.0
62	26352.5
63	22497.0
64	22497.0
65	22497.0
66	18270.5
67	14044.0
68	14044.0
69	14044.0
70	10654.0
71	7264.0
72	7264.0
73	7264.0
74	5529.5
75	3795.0
76	3795.0
77	3795.0
78	2988.5
79	2182.0
80	2182.0
81	2182.0
82	1526.0
83	870.0
84	870.0
85	870.0
86	664.0
87	458.0
88	458.0
89	458.0
90	308.0
91	158.0
92	158.0
93	158.0
94	94.0
95	30.0
96	30.0
97	30.0
98	83.5
99	137.0
100	137.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010466628463472775
2	7.849971347604582E-4
3	2.6166571158681937E-4
4	0.008896634193951859
5	0.03663319962215471
6	0.06803308501257303
7	0.10518961605790139
8	0.11356291882867961
9	0.12219788731104464
10	0.14077615283370884
11	0.1402528214105352
12	0.1378978300062538
13	0.1284778643891283
14	0.12088955875311055
15	0.13423451004403836
16	0.12219788731104464
17	0.12952452723547558
18	0.11591791023296098
19	0.099956301826165
20	0.09864797326823091
21	0.09943297040299136
22	0.10492795034631457
23	0.08818134480475813
24	0.09812464184505726
25	0.09419965617125498
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	382167.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.857640823170705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	84.36371353741067	53.02904004043424
2	9.953004805837912	12.512448023933025
3	2.464979527030167	4.648283932395944
4	0.9781399652023622	2.4593428242991506
5	0.5333243683194696	1.6761755793034807
6	0.29691943638459684	1.1198193171408763
7	0.21036981215843323	0.925634506488488
8	0.1526874901237967	0.7678060329914428
9	0.11469461552300855	0.6488489652207237
>10	0.7775375874222723	9.8043428779587
>50	0.09486689186870595	4.099309082835088
>100	0.05516488866329028	5.979395920724839
>500	0.0037612424088607	1.688584350163398
>1k	8.358316464134889E-4	0.6409685461105618
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACATGGAAGCAGTGGTATCAACG	1369	0.35822035916235573	No Hit
TCCATGTACTCTGCGTTGATACCAC	1071	0.28024397710948357	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	966	0.2527690773928675	No Hit
CTGTAGGACGTGGAATATGGCAAGA	914	0.23916246039035288	No Hit
GTCCTACAGTGGACATTTCTAAATT	852	0.2229391862719701	No Hit
GTATCAACGCAGAGTACTTTTTTTT	805	0.2106408978273896	No Hit
CTTTAGGACGTGAAATATGGCGAGG	778	0.20357592361454546	No Hit
GTCCTACAGTGTGCATTTCTCATTT	548	0.14339280994957704	No Hit
TATCAACGCAGAGTACTTTTTTTTT	530	0.13868282714101426	No Hit
GAGTACATGGAAGCAGTGGTATCAA	529	0.13842116142942745	No Hit
GTACATGGGAAGCAGTGGTATCAAC	506	0.13240285006293062	No Hit
CCCATGTACTCTGCGTTGATACCAC	452	0.11827290163724236	No Hit
CTGAAGGACCTGGAATATGGCGAGA	439	0.1148712473866137	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	424	0.11094626171281141	No Hit
GCTTCCATGTACTCTGCGTTGATAC	399	0.10440461892314093	No Hit
GTCCTTCAGTGTGCATTTCTCATTT	386	0.10100296467251228	No Hit
CTGTAGGACCTGGAATATGGCGAGA	385	0.10074129896092546	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTTAAG	35	9.9382996E-5	18.994102	1
CGCCACT	40	2.7552858E-4	16.624197	17
TCGCCAG	45	6.7482685E-4	14.777064	17
GTCTAAG	45	6.761697E-4	14.773191	1
CCGTCGT	40	0.005261972	14.253049	9
CGAGCCG	40	0.0052711354	14.249312	15
CTACACC	50	0.0014964782	13.299358	4
GATATAC	110	2.730303E-9	12.950524	1
TATACAC	125	1.0186341E-10	12.917683	3
TAGGACC	180	0.0	12.666055	4
TGAGTTA	85	3.9119623E-6	12.296747	12
TAGAGTG	55	0.0030622638	12.090325	5
AATGCCG	55	0.0030622638	12.090325	18
GCCGTTT	55	0.0030622638	12.090325	15
ATGCCGG	55	0.003065272	12.088739	19
TAGAAAT	95	1.0335516E-6	11.999421	4
CACGCTG	65	7.9847535E-4	11.694809	10
GTCTTAT	65	8.0023415E-4	11.691743	16
CGTTTCT	65	8.0023415E-4	11.691743	17
TACACTG	180	0.0	11.610551	5
>>END_MODULE