FastQCFastQC Report
Thu 2 Feb 2017
SRR4062096_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062096_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382167
Sequences flagged as poor quality0
Sequence length50
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5190.13580450431355925No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA4120.10780627317376959No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCTATTA250.002349825835.20293442
GCAACCG250.00235133535.19832612
ATAGGGG353.2146744E-431.4270763
GGACGTC353.2146744E-431.4270766
CGAGCCG801.2914825E-1030.24856215
TCCTATA454.063669E-529.3319382
GGACCGT300.00574149729.3319386
CGTCTTA801.3055433E-724.74882315
ATAGGGT1102.0008883E-1023.998863
AACCGCG752.056633E-623.46862241
TAGTGAC752.058976E-623.46555118
GTCCTAT1053.0413503E-923.0555741
TTAGGAC3750.022.8789123
CGACCCG602.868726E-421.9989535
GCCGCCT1105.2277755E-921.99895318
GATTAAT904.0685882E-721.99895338
CCGAGCC1259.913492E-1021.11899614
ACCGCAG1158.714778E-921.0452329
TGGATAC1158.714778E-921.0452324
TAGGGTC1158.72933E-921.0424774
Produced by FastQC (version 0.11.5)