##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062096_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	382167
Sequences flagged as poor quality	0
Sequence length	50
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.209649184780474	32.0	32.0	32.0	32.0	32.0
2	31.506126902636804	32.0	32.0	32.0	32.0	32.0
3	31.619726454665106	32.0	32.0	32.0	32.0	32.0
4	31.693100136851168	32.0	32.0	32.0	32.0	32.0
5	31.680194783955706	32.0	32.0	32.0	32.0	32.0
6	35.334636428577035	36.0	36.0	36.0	36.0	36.0
7	35.34540658926595	36.0	36.0	36.0	36.0	36.0
8	35.30813230865041	36.0	36.0	36.0	36.0	36.0
9	35.37332893734938	36.0	36.0	36.0	36.0	36.0
10	35.26314935617152	36.0	36.0	36.0	36.0	36.0
11	35.37489631496178	36.0	36.0	36.0	36.0	36.0
12	35.30286759453328	36.0	36.0	36.0	36.0	36.0
13	35.31146592981602	36.0	36.0	36.0	36.0	36.0
14	35.284467261694495	36.0	36.0	36.0	36.0	36.0
15	35.26838790371748	36.0	36.0	36.0	36.0	36.0
16	35.26327495571308	36.0	36.0	36.0	36.0	36.0
17	35.25433645500527	36.0	36.0	36.0	36.0	36.0
18	35.24189948373355	36.0	36.0	36.0	36.0	36.0
19	35.21919213328205	36.0	36.0	36.0	36.0	36.0
20	35.20738839303237	36.0	36.0	36.0	36.0	36.0
21	35.18619085373672	36.0	36.0	36.0	36.0	36.0
22	35.13818827894612	36.0	36.0	36.0	36.0	36.0
23	35.1039859537846	36.0	36.0	36.0	36.0	36.0
24	35.08441597521502	36.0	36.0	36.0	36.0	36.0
25	35.039974670759115	36.0	36.0	36.0	36.0	36.0
26	35.00125599541562	36.0	36.0	36.0	36.0	36.0
27	34.94695774360424	36.0	36.0	36.0	36.0	36.0
28	34.91202798776451	36.0	36.0	36.0	36.0	36.0
29	34.869549176145505	36.0	36.0	36.0	36.0	36.0
30	34.819924273943066	36.0	36.0	36.0	32.0	36.0
31	34.82036125568142	36.0	36.0	36.0	32.0	36.0
32	34.78050433449251	36.0	36.0	36.0	32.0	36.0
33	34.780928232945286	36.0	36.0	36.0	32.0	36.0
34	34.74912538235902	36.0	36.0	36.0	32.0	36.0
35	34.713489129098015	36.0	36.0	36.0	32.0	36.0
36	34.655535407295766	36.0	36.0	36.0	32.0	36.0
37	34.56914908927249	36.0	36.0	36.0	32.0	36.0
38	34.55018355849668	36.0	36.0	36.0	32.0	36.0
39	34.37753390533458	36.0	36.0	36.0	32.0	36.0
40	34.41264944382953	36.0	36.0	36.0	32.0	36.0
41	34.39371792959622	36.0	36.0	36.0	32.0	36.0
42	34.31085624870803	36.0	36.0	36.0	32.0	36.0
43	34.33310045085002	36.0	36.0	36.0	32.0	36.0
44	34.265554064061	36.0	36.0	36.0	32.0	36.0
45	34.18413416124365	36.0	36.0	36.0	32.0	36.0
46	34.15325237396217	36.0	36.0	36.0	32.0	36.0
47	34.105948446621504	36.0	36.0	36.0	32.0	36.0
48	34.10633832853177	36.0	36.0	36.0	32.0	36.0
49	34.09938325391779	36.0	36.0	36.0	32.0	36.0
50	33.70563654109329	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
18	1.0
19	0.0
20	1.0
21	15.0
22	59.0
23	113.0
24	303.0
25	741.0
26	1596.0
27	2803.0
28	4430.0
29	6188.0
30	8784.0
31	11996.0
32	17498.0
33	26760.0
34	59279.0
35	241600.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.78322048049919	18.388948361649057	12.470259984662135	26.35757117318962
2	14.507434409198675	20.31653155109881	39.74255643190227	25.433477607800242
3	18.37809125330026	24.518338841396563	29.31807298903359	27.785496916269587
4	11.922013151318664	16.684590767910365	36.66800116179576	34.72539491897521
5	13.34809127946683	37.639827614629205	34.433114319132734	14.578966786771227
6	33.3338566647565	35.89634897832623	17.36544494945927	13.404349407457996
7	29.026839052037463	30.995088534593517	21.225537526788028	18.752534886580996
8	26.63861610238456	35.63703825814369	19.69243812260085	18.031907516870895
9	27.022479701282425	14.676306431481526	19.268539669830208	39.032674197405846
10	15.956375092564246	27.651000740513965	32.43660493972531	23.95601922719649
11	35.538651950587045	21.133169530597883	24.14677353094328	19.181404987871794
12	25.01053204489137	23.70822179832377	30.044980335821776	21.236265820963087
13	29.834077772282797	20.724447689099268	25.5657866848786	23.875687853739333
14	22.489382913752365	21.32968047999958	25.764390959972992	30.416545646275058
15	23.897667773512627	30.10437845235198	22.7049954600999	23.292958314035488
16	23.875414417408276	26.03810415974336	26.986913960796834	23.09956746205153
17	22.402248231793955	27.396661668851575	27.362383460633705	22.83870663872077
18	23.19253936770932	25.099564063783802	29.885704118105743	21.822192450401133
19	25.191081385886278	25.66521965528159	26.21576431246026	22.927934646371874
20	25.10787168960167	25.565001687743838	27.213234005029214	22.11389261762528
21	24.588988583525005	25.203118008619267	25.30673763040765	24.90115577744808
22	24.632425091648415	27.07612117215772	25.573113324803032	22.71834041139083
23	22.557736690338754	26.79071398292888	26.83545893669243	23.81609039003993
24	24.143164417184334	26.177118604152167	26.996928004354174	22.68278897430932
25	23.908458627926084	25.800306673016436	27.007112092650836	23.28412260640664
26	22.227717202165547	26.81550212341725	27.78811357338546	23.168667101031748
27	23.43582779256189	26.850565328769882	26.681267613373212	23.032339265295015
28	22.703080352937484	26.381867470510045	26.47920787933976	24.435844297212714
29	23.463302692278507	26.063998199739903	26.315982279998014	24.15671682798358
30	23.27212876248492	25.792270796863104	27.420380415584006	23.51522002506797
31	24.366049397253033	24.99744875931203	26.52505318355588	24.11144865987906
32	23.015652883825354	26.734455707729104	26.765855675282467	23.484035733163076
33	22.46583299970955	26.726797447189316	27.625357500778456	23.182012052322676
34	23.0305678684132	26.57562420518832	27.97370776050198	22.420100165896496
35	23.486914063522136	26.75852901618668	27.281076809553962	22.473480110737217
36	22.537791070395926	26.746160709846745	27.44297649980244	23.27307171995489
37	23.653981013386925	26.330580588438472	26.134853099716356	23.880585298458254
38	23.5151125031727	27.315283632548077	25.860422276125362	23.30918158815387
39	24.299515391297977	25.755958175024336	26.221988465684888	23.7225379679928
40	25.10499336677421	25.878738875936435	26.82021210622581	22.19605565106354
41	24.140944725301182	25.36502653311144	27.517505573523408	22.976523168063974
42	23.98534606829779	27.320685594661782	26.793405730734005	21.900562606306423
43	22.80916605826523	26.833512582788387	27.116648218365086	23.240673140581293
44	22.748195075742707	27.0287243980751	26.850259977129337	23.372820549052857
45	22.989444156605384	27.243182139324574	26.5905724857258	23.176801218344245
46	23.29471117112025	25.961890706607633	26.774369119016967	23.969029003255145
47	24.801130416579444	25.61466401507222	26.71237178145279	22.87183378689554
48	23.208042872992944	27.283907973790534	25.71777721953568	23.79027193368084
49	23.578961140913254	26.32552662567055	26.463953944786077	23.63155828863012
50	22.709276980120944	28.509450205541643	25.1231810676652	23.658091746672213
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	144.0
1	93.5
2	43.0
3	149.0
4	255.0
5	238.5
6	222.0
7	164.0
8	106.0
9	144.5
10	183.0
11	282.5
12	382.0
13	622.0
14	862.0
15	1058.5
16	1255.0
17	1278.0
18	1301.0
19	1266.0
20	1231.0
21	1425.5
22	1620.0
23	1832.5
24	2045.0
25	2630.5
26	3216.0
27	4516.5
28	5817.0
29	7033.0
30	8249.0
31	9574.5
32	10900.0
33	13288.5
34	15677.0
35	18031.0
36	20385.0
37	21427.5
38	22470.0
39	21882.0
40	21294.0
41	21261.0
42	21228.0
43	20830.5
44	20433.0
45	21758.0
46	23083.0
47	24309.0
48	25535.0
49	25042.0
50	24549.0
51	23975.5
52	23402.0
53	23007.0
54	22612.0
55	22538.0
56	22464.0
57	21550.0
58	20636.0
59	18891.0
60	17146.0
61	15087.5
62	13029.0
63	11280.0
64	9531.0
65	7954.5
66	6378.0
67	5531.0
68	4684.0
69	4099.0
70	3514.0
71	2840.0
72	2166.0
73	1863.5
74	1561.0
75	1214.0
76	867.0
77	756.0
78	645.0
79	551.5
80	458.0
81	357.5
82	257.0
83	213.5
84	170.0
85	132.0
86	94.0
87	62.0
88	30.0
89	25.5
90	21.0
91	12.5
92	4.0
93	5.5
94	7.0
95	5.0
96	3.0
97	2.5
98	2.0
99	1.5
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.027736565428202853
2	0.006541642789670485
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0010466628463472775
17	0.0
18	2.6166571158681937E-4
19	0.0
20	0.0
21	0.0
22	0.0
23	2.6166571158681937E-4
24	0.0013083285579340968
25	2.6166571158681937E-4
26	0.0
27	0.0
28	7.849971347604582E-4
29	0.0
30	0.0015699942695209163
31	0.0
32	2.6166571158681937E-4
33	0.0
34	2.6166571158681937E-4
35	2.6166571158681937E-4
36	0.0
37	7.849971347604582E-4
38	0.0
39	7.849971347604582E-4
40	0.0
41	7.849971347604582E-4
42	0.004448317096975929
43	0.004709982808562749
44	0.004709982808562749
45	0.0023549914042813743
46	7.849971347604582E-4
47	0.0018316599811077354
48	0.003924985673802291
49	0.004448317096975929
50	0.0015699942695209163
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	382167.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	69.3199567904096
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.21148621001329	59.068565421247044
2	9.737916004150144	13.500638332726522
3	2.305952852124707	4.7954565621001946
4	0.8948595641228754	2.4812650527393
5	0.45516230973908917	1.5775915821868345
6	0.2762314314839918	1.1489010536773983
7	0.17829200158901687	0.8651435692358402
8	0.12538343010210726	0.6953259165529134
9	0.09803233353897496	0.611603741248629
>10	0.6159007780977339	8.36614459284902
>50	0.0700934989951368	3.4232767590441053
>100	0.03031070226470906	3.3297761813305957
>500	3.7888377830886327E-4	0.1363112350615696
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	519	0.13580450431355925	No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA	412	0.10780627317376959	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	5.233314231736387E-4	0.0
18	0.0	0.0	0.0	5.233314231736387E-4	0.0
19	0.0	0.0	0.0	5.233314231736387E-4	0.0
20	0.0	0.0	0.0	5.233314231736387E-4	0.0
21	0.0	0.0	0.0	0.0015699942695209163	0.0
22	0.0	0.0	0.0	0.0023549914042813743	0.0
23	0.0	0.0	0.0	0.003401654250628652	2.6166571158681937E-4
24	0.0	0.0	0.0	0.003924985673802291	2.6166571158681937E-4
25	0.0	0.0	0.0	0.004709982808562749	2.6166571158681937E-4
26	0.0	0.0	0.0	0.005233314231736387	2.6166571158681937E-4
27	0.0	0.0	0.0	0.007064974212844123	2.6166571158681937E-4
28	0.0	0.0	0.0	0.009419965617125497	5.233314231736387E-4
29	0.0	0.0	0.0	0.01308328557934097	5.233314231736387E-4
30	0.0	0.0	0.0	0.0175316026763169	5.233314231736387E-4
31	0.0	0.0	0.0	0.029829891120897407	5.233314231736387E-4
32	0.0	0.0	0.0	0.04108151671913064	7.849971347604582E-4
33	0.0	0.0	0.0	0.05102481375942978	7.849971347604582E-4
34	0.0	0.0	0.0	0.06646309074305212	7.849971347604582E-4
35	0.0	0.0	0.0	0.0837330277077822	7.849971347604582E-4
36	0.0	0.0	0.0	0.10388128749996729	7.849971347604582E-4
37	0.0	0.0	0.0	0.13214118435134378	7.849971347604582E-4
38	0.0	0.0	0.0	0.16380273545334892	7.849971347604582E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCTATTA	25	0.0023498258	35.202934	42
GCAACCG	25	0.002351335	35.198326	12
ATAGGGG	35	3.2146744E-4	31.427076	3
GGACGTC	35	3.2146744E-4	31.427076	6
CGAGCCG	80	1.2914825E-10	30.248562	15
TCCTATA	45	4.063669E-5	29.331938	2
GGACCGT	30	0.005741497	29.331938	6
CGTCTTA	80	1.3055433E-7	24.748823	15
ATAGGGT	110	2.0008883E-10	23.99886	3
AACCGCG	75	2.056633E-6	23.468622	41
TAGTGAC	75	2.058976E-6	23.465551	18
GTCCTAT	105	3.0413503E-9	23.055574	1
TTAGGAC	375	0.0	22.878912	3
CGACCCG	60	2.868726E-4	21.998953	5
GCCGCCT	110	5.2277755E-9	21.998953	18
GATTAAT	90	4.0685882E-7	21.998953	38
CCGAGCC	125	9.913492E-10	21.118996	14
ACCGCAG	115	8.714778E-9	21.04523	29
TGGATAC	115	8.714778E-9	21.04523	24
TAGGGTC	115	8.72933E-9	21.042477	4
>>END_MODULE