FastQCFastQC Report
Thu 2 Feb 2017
SRR4062095_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062095_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377946
Sequences flagged as poor quality0
Sequence length25
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTAT16300.4312785424372794No Hit
GTATTAGAGGCACTGCCTGCCCAGT10420.27570076148444483No Hit
GTATCAACGCAGAGTACTTTTTTTT9200.24342101781736014No Hit
GTCAGGATACCGCGGCCGTTAAACT8730.23098537886364717No Hit
GATATACACTGTTCTACAAATCCCG8280.21907891603562415No Hit
CTCTAATACTTGTAATGCTAGAGGT8020.21219962640165527No Hit
CCATTGGGATGTCCTGATCCAACAT7840.20743704127044604No Hit
TTGTAGAACAGTGTATATCAATGAG7820.20690786514475615No Hit
CTTCTACACCATTGGGATGTCCTGA7760.20532033676768638No Hit
ATAAATAATCCACCTATAACTTCTC7640.20214528001354692No Hit
ACCTATAACTTCTCTGTTAACCCAA7620.201616103887857No Hit
GTCCTACAGTGGACATTTCTAAATT7470.19764728294518266No Hit
GTTATATAATTTAAGCTCCATAGGG7440.1968535187566478No Hit
CCTATAACTTCTCTGTTAACCCAAC7420.19632434263095788No Hit
GTCCTACAGTGTGCATTTCTCATTT7350.19447222619104315No Hit
ATCCTGACCGTGCAAAGGTAGCATA7320.1936784620025083No Hit
GTCCTAAAGTGTGTATTTCTCATTT7310.19341387393966333No Hit
CTGTAGGACGTGGAATATGGCAAGA7310.19341387393966333No Hit
GACTATAGGCAATAATCACACTATA7190.19023881718552388No Hit
GCCTAAAGGAAAGATCCAAAAAGAT7140.18891587687129907No Hit
GTTCATGCTAGTCCCTAATTAAGGA7050.18653458430569447No Hit
GTGTATATCAATGAGTTACAATGAA6990.18494705592862473No Hit
CTGTTAGTATGAGTAACAAGAATTC6990.18494705592862473No Hit
CTATAGAACTAGTACCGCAAGGGAA6920.18309493948871003No Hit
GATTAAACCTTGTACCTTTTGCATA6780.17939070660888062No Hit
CACTATAAATAATCCACCTATAACT6690.17700941404327603No Hit
ATCTATAACTTTATAGATGCAACAC6670.1764802379175861No Hit
GGTCAGGATACCGCGGCCGTTAAAC6520.17251141697491176No Hit
CCTCTAGCATTACAAGTATTAGAGG6320.16721965571801262No Hit
CTTTAGGACGTGAAATATGGCGAGG6280.1661613034666328No Hit
TATCAACGCAGAGTACTTTTTTTTT6250.1653675392780979No Hit
GATTAAAGATAAGAGACAGTTGGAC6240.16510295121525295No Hit
GTACATGGGAGAAATCGTAAATAGA6120.1619278944611135No Hit
CTGTTAACCCAACACCGGAATGCCT6010.15901742576981895No Hit
CTGTAGGACCTGGAATATGGCGAGA5960.1576944854555942No Hit
ATCGTAAATAGATAGAAACCGACCT5950.15742989739274924No Hit
GTATCAACGCAGAGTACATGGGAGA5930.1569007212670593No Hit
GTTATAGATTAACCCAATTTTAAGT5920.15663613320421435No Hit
GTATCCTGACCGTGCAAAGGTAGCA5540.146581786816106No Hit
CTATTAAAGGTTTTTTCCGTTCCAG5340.14129002555920686No Hit
CATTTACACCTACTACCCAACTATC5050.13361697173670314No Hit
TACTAACAGTGTTGCATCTATAAAG5030.13308779561101322No Hit
GGATACCGCGGCCGTTAAACTTTAG5020.13282320754816826No Hit
GTGTATATCAATGAGTTACAATGAG5000.13229403142247834No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA4970.13150026723394348No Hit
GATATATTTTGATCAACGGACCAAG4960.13123567917109852No Hit
AAATATATCTGGGTCAATAAGATAT4920.1301773269197187No Hit
GTTAGTATGAGTAACAAGAATTCCA4900.12964815079402878No Hit
TGCTAGAGGTGATGTTTTTGGTAAA4880.12911897466833885No Hit
GATAGAAACCGACCTGGATTGCTCC4860.12858979854264896No Hit
GTCCTGATCCAACATCGAGGTCGTA4650.12303344922290485No Hit
GTTCTACAGTGTGGTTTTTATCATT4650.12303344922290485No Hit
GATATACACTGTTCTACAATGCCGG4590.12144592084583512No Hit
GTTTAAAATTGAACTTAAATTCATT4590.12144592084583512No Hit
AGCATGAACGGCTAAACGAGGGTCC4530.11985839246876537No Hit
GTATAGGGGTCCTAGGAAGATAATA4520.11959380440592043No Hit
CTTTATTGGTGGCTGCTTTTAGGCC4490.11880004021738555No Hit
GTATTGGAATTAGTGAAATTGGAGT4450.11774168796600572No Hit
ACCTTTGCACGGTCAGGATACCGCG4440.11747709990316077No Hit
GACCTGGATTGCTCCGGTCTGAACT4410.11668333571462589No Hit
AATCTAAACTTACTTTTTGATTTTG4210.11139157445772677No Hit
GCTATCACCAAGCTCGTTAGGCTTT4140.10953945801781208No Hit
TATCAACGCAGAGTACATGGGAGAA4130.10927486995496712No Hit
GTGTAAATGTATGTGGTAAAAGGCC3990.10557063707513771No Hit
CTTAAATAGGATTGCGCTGTTATCC3970.1050414609494478No Hit
ATTATAACCTAGACTTACAAGTCAA3870.10239558032099824No Hit
GATATAGGCTTACTAGGAGGGTGAA3820.10107264000677346No Hit
CGTCTATGTGGCAAAATAGTGAGAA3800.10054346388108354No Hit
GGGCAGGCAGTGCCTCTAATACTTG3790.10027887581823859No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGGAAT250.00602380319.0000027
TTGCAAT250.00602380319.00000216
ATTCTGC250.00602380319.0000026
ATTTACC359.914286E-519.017
CCCTTTA402.756959E-416.6227971
TTACCAG402.756959E-416.62279719
CCAACGA651.9049185E-716.07479319
GAACATA901.3278623E-1015.8333336
CAATTTA508.6861095E-515.20000115
TAGGACC2800.014.5892864
ACCTACT851.7029379E-814.5294118
TCCAACG1058.731149E-1114.47427318
AGGACCT2850.014.3333335
CAACCTC400.005264677614.2518889
GAACAGT1203.6379788E-1214.2500016
GTTCGTT400.005273943814.24811219
TTAGGAC2900.014.0862073
CACCTAC954.8567017E-914.07
ATATATC954.8567017E-914.03
TTTAAAA954.8657967E-913.9981462
Produced by FastQC (version 0.11.5)