##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062095_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	377946
Sequences flagged as poor quality	0
Sequence length	25
%GC	40
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.293211199483522	32.0	32.0	32.0	32.0	32.0
2	30.960449376365936	32.0	32.0	32.0	32.0	32.0
3	31.004238700766777	32.0	32.0	32.0	32.0	32.0
4	30.99643335291285	32.0	32.0	32.0	32.0	32.0
5	30.99670587861758	32.0	32.0	32.0	32.0	32.0
6	34.614593090018154	36.0	36.0	36.0	32.0	36.0
7	34.52075164176893	36.0	36.0	36.0	32.0	36.0
8	34.46517491916835	36.0	36.0	36.0	32.0	36.0
9	34.56428431574881	36.0	36.0	36.0	32.0	36.0
10	34.36184005122425	36.0	36.0	36.0	32.0	36.0
11	34.58062792039075	36.0	36.0	36.0	32.0	36.0
12	34.44493657824134	36.0	36.0	36.0	32.0	36.0
13	34.528239483947445	36.0	36.0	36.0	32.0	36.0
14	34.46953533044403	36.0	36.0	36.0	32.0	36.0
15	34.417305646838436	36.0	36.0	36.0	32.0	36.0
16	34.44926523894948	36.0	36.0	36.0	32.0	36.0
17	34.4032771877464	36.0	36.0	36.0	32.0	36.0
18	34.41787186529292	36.0	36.0	36.0	32.0	36.0
19	34.37165097659454	36.0	36.0	36.0	32.0	36.0
20	34.350415138670606	36.0	36.0	36.0	32.0	36.0
21	34.3105073211517	36.0	36.0	36.0	32.0	36.0
22	34.28494017663899	36.0	36.0	36.0	32.0	36.0
23	34.29123208077344	36.0	36.0	36.0	32.0	36.0
24	34.26447958173919	36.0	36.0	36.0	32.0	36.0
25	33.92152053467956	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
3	1.0
4	4.0
5	44.0
6	76.0
7	21.0
8	71.0
9	50.0
10	30.0
11	11.0
12	18.0
13	9.0
14	65.0
15	84.0
16	152.0
17	193.0
18	231.0
19	311.0
20	483.0
21	638.0
22	951.0
23	1310.0
24	1759.0
25	2521.0
26	3412.0
27	4461.0
28	6209.0
29	8208.0
30	10985.0
31	15144.0
32	22377.0
33	33076.0
34	79541.0
35	185500.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.14145556725635	23.352524990607023	13.202819480237709	24.303199961898915
2	13.2915813370164	22.79743667084716	43.02969207241246	20.88128991972398
3	16.509411497002187	27.505013996856658	32.484349450444775	23.501225055696377
4	9.510238212427693	19.801904217540184	43.55090527073443	27.136952299297697
5	9.75997755414269	41.66538732338446	38.22836542279207	10.346269699680782
6	25.861790490715514	44.344710900699816	19.222184270519733	10.571314338064939
7	24.58061046904345	35.38209280210218	22.980400674943642	17.056896053910727
8	22.269549761463907	39.91274426440554	21.11779524305252	16.699910731078035
9	24.407948942799084	16.792817233129956	23.157217075232012	35.64201674883895
10	13.600991995675916	28.896908227152174	36.51042204858766	20.99167772858426
11	30.453330224399654	25.34346209794731	27.75915656042416	16.44405111722888
12	20.633520130360477	29.290833697653778	32.79307923638435	17.28256693560139
13	25.037687410417885	23.464486404713774	29.54454532689358	21.95328085797476
14	18.569657408682936	26.497578654007143	29.22967287986521	25.70309105744471
15	21.665796745878033	33.49027234261278	24.71617178330229	20.127759128206897
16	20.49110407019254	30.245896427852365	29.326102851572024	19.93689665038307
17	17.411289083404423	31.469392945515047	29.884332727532836	21.234985243547694
18	17.413198905433365	31.26826154102903	32.40786117122869	18.91067838230892
19	21.36441771089092	30.018037202723857	28.766292414574906	19.851252671810315
20	19.634888223251576	32.09095748484439	29.80430792699883	18.469846364905198
21	21.43042534032523	28.763440860215056	28.208856401292444	21.597277398167275
22	19.952058060073107	31.16755840440748	29.087249033215024	19.79313450230439
23	20.706046225875475	31.04172404296337	29.224042963370973	19.028186767790185
24	20.805205604976788	31.344595900877398	29.157320183372043	18.692878310773768
25	20.186017934417023	30.83146626835947	30.765787954385353	18.21672784283815
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	553.0
1	553.0
2	402.5
3	252.0
4	252.0
5	252.0
6	836.0
7	1420.0
8	1420.0
9	1420.0
10	1464.5
11	1509.0
12	1509.0
13	1509.0
14	2231.5
15	2954.0
16	2954.0
17	2954.0
18	5653.0
19	8352.0
20	8352.0
21	8352.0
22	12274.5
23	16197.0
24	16197.0
25	16197.0
26	24386.5
27	32576.0
28	32576.0
29	32576.0
30	40139.0
31	47702.0
32	47702.0
33	47702.0
34	49517.0
35	51332.0
36	51332.0
37	51332.0
38	50221.0
39	49110.0
40	49110.0
41	49110.0
42	45215.5
43	41321.0
44	41321.0
45	41321.0
46	43673.5
47	46026.0
48	46026.0
49	46026.0
50	39497.0
51	32968.0
52	32968.0
53	32968.0
54	26954.0
55	20940.0
56	20940.0
57	20940.0
58	16220.0
59	11500.0
60	11500.0
61	11500.0
62	8886.5
63	6273.0
64	6273.0
65	6273.0
66	4903.5
67	3534.0
68	3534.0
69	3534.0
70	2645.5
71	1757.0
72	1757.0
73	1757.0
74	1317.0
75	877.0
76	877.0
77	877.0
78	649.0
79	421.0
80	421.0
81	421.0
82	276.5
83	132.0
84	132.0
85	132.0
86	97.0
87	62.0
88	62.0
89	62.0
90	49.0
91	36.0
92	36.0
93	36.0
94	24.5
95	13.0
96	13.0
97	13.0
98	71.0
99	129.0
100	129.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0010583522513798267
2	0.0
3	0.0010583522513798267
4	0.01270022701655792
5	0.03915903330105359
6	0.07223254115667317
7	0.11456663121186625
8	0.11509580733755616
9	0.1288543866054939
10	0.13890873299360226
11	0.14261296587343167
12	0.140496261370672
13	0.13202944335963337
14	0.12435638953712964
15	0.13679202849084263
16	0.12435638953712964
17	0.12806062241695904
18	0.11774168796600572
19	0.10398310869806798
20	0.09604546681271928
21	0.09683923100125415
22	0.10742275351505243
23	0.08519735623607605
24	0.09339958618426972
25	0.09207664587004494
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	377946.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	27.12767504850492
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.19865285468431	18.50070893387668
2	15.333160374898751	8.319059842337305
3	5.301816308955475	4.314778499886236
4	2.5944781814786713	2.8152864411035745
5	1.4932123785102986	2.0253690091316257
6	0.9492151704330664	1.5450000416771665
7	0.7176495398287083	1.36277144606276
8	0.5509631200136741	1.1957078786753093
9	0.4222054271058572	1.0308106467218632
>10	3.472464959496032	19.692626049269975
>50	0.5379875289223124	10.30891874719345
>100	0.38527492794807533	20.572348608574682
>500	0.04092298457479557	7.593123957333174
>1k	0.0019962431499900278	0.7234898981561974
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCATAGGGTCTTCTCGTCTTATTAT	1630	0.4312785424372794	No Hit
GTATTAGAGGCACTGCCTGCCCAGT	1042	0.27570076148444483	No Hit
GTATCAACGCAGAGTACTTTTTTTT	920	0.24342101781736014	No Hit
GTCAGGATACCGCGGCCGTTAAACT	873	0.23098537886364717	No Hit
GATATACACTGTTCTACAAATCCCG	828	0.21907891603562415	No Hit
CTCTAATACTTGTAATGCTAGAGGT	802	0.21219962640165527	No Hit
CCATTGGGATGTCCTGATCCAACAT	784	0.20743704127044604	No Hit
TTGTAGAACAGTGTATATCAATGAG	782	0.20690786514475615	No Hit
CTTCTACACCATTGGGATGTCCTGA	776	0.20532033676768638	No Hit
ATAAATAATCCACCTATAACTTCTC	764	0.20214528001354692	No Hit
ACCTATAACTTCTCTGTTAACCCAA	762	0.201616103887857	No Hit
GTCCTACAGTGGACATTTCTAAATT	747	0.19764728294518266	No Hit
GTTATATAATTTAAGCTCCATAGGG	744	0.1968535187566478	No Hit
CCTATAACTTCTCTGTTAACCCAAC	742	0.19632434263095788	No Hit
GTCCTACAGTGTGCATTTCTCATTT	735	0.19447222619104315	No Hit
ATCCTGACCGTGCAAAGGTAGCATA	732	0.1936784620025083	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	731	0.19341387393966333	No Hit
CTGTAGGACGTGGAATATGGCAAGA	731	0.19341387393966333	No Hit
GACTATAGGCAATAATCACACTATA	719	0.19023881718552388	No Hit
GCCTAAAGGAAAGATCCAAAAAGAT	714	0.18891587687129907	No Hit
GTTCATGCTAGTCCCTAATTAAGGA	705	0.18653458430569447	No Hit
GTGTATATCAATGAGTTACAATGAA	699	0.18494705592862473	No Hit
CTGTTAGTATGAGTAACAAGAATTC	699	0.18494705592862473	No Hit
CTATAGAACTAGTACCGCAAGGGAA	692	0.18309493948871003	No Hit
GATTAAACCTTGTACCTTTTGCATA	678	0.17939070660888062	No Hit
CACTATAAATAATCCACCTATAACT	669	0.17700941404327603	No Hit
ATCTATAACTTTATAGATGCAACAC	667	0.1764802379175861	No Hit
GGTCAGGATACCGCGGCCGTTAAAC	652	0.17251141697491176	No Hit
CCTCTAGCATTACAAGTATTAGAGG	632	0.16721965571801262	No Hit
CTTTAGGACGTGAAATATGGCGAGG	628	0.1661613034666328	No Hit
TATCAACGCAGAGTACTTTTTTTTT	625	0.1653675392780979	No Hit
GATTAAAGATAAGAGACAGTTGGAC	624	0.16510295121525295	No Hit
GTACATGGGAGAAATCGTAAATAGA	612	0.1619278944611135	No Hit
CTGTTAACCCAACACCGGAATGCCT	601	0.15901742576981895	No Hit
CTGTAGGACCTGGAATATGGCGAGA	596	0.1576944854555942	No Hit
ATCGTAAATAGATAGAAACCGACCT	595	0.15742989739274924	No Hit
GTATCAACGCAGAGTACATGGGAGA	593	0.1569007212670593	No Hit
GTTATAGATTAACCCAATTTTAAGT	592	0.15663613320421435	No Hit
GTATCCTGACCGTGCAAAGGTAGCA	554	0.146581786816106	No Hit
CTATTAAAGGTTTTTTCCGTTCCAG	534	0.14129002555920686	No Hit
CATTTACACCTACTACCCAACTATC	505	0.13361697173670314	No Hit
TACTAACAGTGTTGCATCTATAAAG	503	0.13308779561101322	No Hit
GGATACCGCGGCCGTTAAACTTTAG	502	0.13282320754816826	No Hit
GTGTATATCAATGAGTTACAATGAG	500	0.13229403142247834	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA	497	0.13150026723394348	No Hit
GATATATTTTGATCAACGGACCAAG	496	0.13123567917109852	No Hit
AAATATATCTGGGTCAATAAGATAT	492	0.1301773269197187	No Hit
GTTAGTATGAGTAACAAGAATTCCA	490	0.12964815079402878	No Hit
TGCTAGAGGTGATGTTTTTGGTAAA	488	0.12911897466833885	No Hit
GATAGAAACCGACCTGGATTGCTCC	486	0.12858979854264896	No Hit
GTCCTGATCCAACATCGAGGTCGTA	465	0.12303344922290485	No Hit
GTTCTACAGTGTGGTTTTTATCATT	465	0.12303344922290485	No Hit
GATATACACTGTTCTACAATGCCGG	459	0.12144592084583512	No Hit
GTTTAAAATTGAACTTAAATTCATT	459	0.12144592084583512	No Hit
AGCATGAACGGCTAAACGAGGGTCC	453	0.11985839246876537	No Hit
GTATAGGGGTCCTAGGAAGATAATA	452	0.11959380440592043	No Hit
CTTTATTGGTGGCTGCTTTTAGGCC	449	0.11880004021738555	No Hit
GTATTGGAATTAGTGAAATTGGAGT	445	0.11774168796600572	No Hit
ACCTTTGCACGGTCAGGATACCGCG	444	0.11747709990316077	No Hit
GACCTGGATTGCTCCGGTCTGAACT	441	0.11668333571462589	No Hit
AATCTAAACTTACTTTTTGATTTTG	421	0.11139157445772677	No Hit
GCTATCACCAAGCTCGTTAGGCTTT	414	0.10953945801781208	No Hit
TATCAACGCAGAGTACATGGGAGAA	413	0.10927486995496712	No Hit
GTGTAAATGTATGTGGTAAAAGGCC	399	0.10557063707513771	No Hit
CTTAAATAGGATTGCGCTGTTATCC	397	0.1050414609494478	No Hit
ATTATAACCTAGACTTACAAGTCAA	387	0.10239558032099824	No Hit
GATATAGGCTTACTAGGAGGGTGAA	382	0.10107264000677346	No Hit
CGTCTATGTGGCAAAATAGTGAGAA	380	0.10054346388108354	No Hit
GGGCAGGCAGTGCCTCTAATACTTG	379	0.10027887581823859	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	2.645880628449567E-4	0.0
9	0.0	0.0	0.0	2.645880628449567E-4	0.0
10	0.0	0.0	0.0	5.291761256899134E-4	0.0
11	0.0	0.0	2.645880628449567E-4	5.291761256899134E-4	0.0
12	0.0	0.0	7.937641885348701E-4	5.291761256899134E-4	0.0
13	0.0	0.0	7.937641885348701E-4	5.291761256899134E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGGAAT	25	0.006023803	19.000002	7
TTGCAAT	25	0.006023803	19.000002	16
ATTCTGC	25	0.006023803	19.000002	6
ATTTACC	35	9.914286E-5	19.0	17
CCCTTTA	40	2.756959E-4	16.622797	1
TTACCAG	40	2.756959E-4	16.622797	19
CCAACGA	65	1.9049185E-7	16.074793	19
GAACATA	90	1.3278623E-10	15.833333	6
CAATTTA	50	8.6861095E-5	15.200001	15
TAGGACC	280	0.0	14.589286	4
ACCTACT	85	1.7029379E-8	14.529411	8
TCCAACG	105	8.731149E-11	14.474273	18
AGGACCT	285	0.0	14.333333	5
CAACCTC	40	0.0052646776	14.251888	9
GAACAGT	120	3.6379788E-12	14.250001	6
GTTCGTT	40	0.0052739438	14.248112	19
TTAGGAC	290	0.0	14.086207	3
CACCTAC	95	4.8567017E-9	14.0	7
ATATATC	95	4.8567017E-9	14.0	3
TTTAAAA	95	4.8657967E-9	13.998146	2
>>END_MODULE