FastQCFastQC Report
Thu 2 Feb 2017
SRR4062095_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062095_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences377946
Sequences flagged as poor quality0
Sequence length50
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG15290.40455514808993875No Hit
GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT10550.2791404063014293No Hit
ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA8310.219872680224159No Hit
CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT7840.20743704127044604No Hit
GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC7660.20267445613923685No Hit
ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC7630.20188069195070196No Hit
ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT7530.1992348113222524No Hit
CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA7360.1947368142538881No Hit
CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA7270.19235552168828352No Hit
GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT7270.19235552168828352No Hit
GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC7260.19209093362543855No Hit
CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC7200.1905034052483688No Hit
GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC7150.18918046493414403No Hit
GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC7020.1857408201171596No Hit
GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC6960.18415329174008988No Hit
CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG6900.1825657633630201No Hit
CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG6850.18124282304879533No Hit
ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC6820.18044905886026044No Hit
CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA6730.17806776629465587No Hit
GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA6210.1643091870267181No Hit
GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC6140.1624570705868034No Hit
GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT5980.1582236615812841No Hit
ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT5850.15478401676429968No Hit
CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT5810.15372566451291983No Hit
CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA5740.15187354807300515No Hit
TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT5660.1497568435702455No Hit
CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA5520.14605261069041608No Hit
CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA5300.14023167330782704No Hit
GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC5270.13943790911929219No Hit
GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA5200.13758579267937748No Hit
GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA5170.13679202849084263No Hit
GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG5150.1362628523651527No Hit
CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT4890.12938356273118382No Hit
GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC4860.12858979854264896No Hit
ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA4810.12726685822842418No Hit
GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA4800.12700227016557922No Hit
GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT4780.1264730940398893No Hit
GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT4690.12409180147428468No Hit
GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG4650.12303344922290485No Hit
GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC4580.12118133278299016No Hit
AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT4520.11959380440592043No Hit
TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT4440.11747709990316077No Hit
GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA4390.11615415958893599No Hit
ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA4240.11218533864626164No Hit
AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG4110.10874569382927719No Hit
GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT4110.10874569382927719No Hit
CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA4030.10662898932651754No Hit
GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG3980.10530604901229276No Hit
GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT3950.10451228482375788No Hit
TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT3950.10451228482375788No Hit
CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA3930.10398310869806798No Hit
TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA3920.10371852063522302No Hit
GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA3910.10345393257237807No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3840.10160181613246336No Hit
GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT3830.10133722806961842No Hit
ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC3830.10133722806961842No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGAG454.062648E-529.3328933
GATAACT300.005740504729.3328924
AGATAAC300.005740504729.3328923
AGACATC300.005740504729.3328938
TAGTCGG554.949792E-627.9995840
ATAGGAC756.986738E-826.3996053
ACCTACT1350.026.073688
GACGTGA1900.025.47337
TACATTG551.5909558E-423.999645
AGGACCT3400.023.9408155
CCTACAG5700.023.9294643
TAGGACC2500.023.7596444
CAGTGAT2800.023.57107437
ACTGTTG752.058292E-623.46631626
GTTATAT1700.023.3091871
AGGGGTC852.3441135E-723.2937665
GTATAGG952.6546331E-823.1728761
TCCTACA6300.023.0472722
GGACCTG3550.022.9292346
GTATCAA6750.022.5034391
Produced by FastQC (version 0.11.5)