##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR4062095_1.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 377946 Sequences flagged as poor quality 0 Sequence length 50 %GC 39 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.23918760881184 32.0 32.0 32.0 32.0 32.0 2 31.462650749048805 32.0 32.0 32.0 32.0 32.0 3 31.600681049673764 32.0 32.0 32.0 32.0 32.0 4 31.671773216279576 32.0 32.0 32.0 32.0 32.0 5 31.66971472115064 32.0 32.0 32.0 32.0 32.0 6 35.260243526853046 36.0 36.0 36.0 36.0 36.0 7 35.27488583025088 36.0 36.0 36.0 36.0 36.0 8 35.23258349076323 36.0 36.0 36.0 36.0 36.0 9 35.32709434681145 36.0 36.0 36.0 36.0 36.0 10 35.22137289454049 36.0 36.0 36.0 36.0 36.0 11 35.334344059733404 36.0 36.0 36.0 36.0 36.0 12 35.2515438713467 36.0 36.0 36.0 36.0 36.0 13 35.314126885851415 36.0 36.0 36.0 36.0 36.0 14 35.277171871113865 36.0 36.0 36.0 36.0 36.0 15 35.25980431066872 36.0 36.0 36.0 36.0 36.0 16 35.274936101982824 36.0 36.0 36.0 36.0 36.0 17 35.2656146645288 36.0 36.0 36.0 36.0 36.0 18 35.265318325898406 36.0 36.0 36.0 36.0 36.0 19 35.23822186238246 36.0 36.0 36.0 36.0 36.0 20 35.23346986077376 36.0 36.0 36.0 36.0 36.0 21 35.19806004032322 36.0 36.0 36.0 36.0 36.0 22 35.17937747720574 36.0 36.0 36.0 36.0 36.0 23 35.18784164933615 36.0 36.0 36.0 36.0 36.0 24 35.16089864689665 36.0 36.0 36.0 36.0 36.0 25 35.158985675202274 36.0 36.0 36.0 36.0 36.0 26 35.133736036364986 36.0 36.0 36.0 36.0 36.0 27 35.090766934958964 36.0 36.0 36.0 36.0 36.0 28 35.06697253046732 36.0 36.0 36.0 36.0 36.0 29 35.03704232879829 36.0 36.0 36.0 36.0 36.0 30 35.01653410804718 36.0 36.0 36.0 36.0 36.0 31 35.02698269064893 36.0 36.0 36.0 36.0 36.0 32 34.98503489916549 36.0 36.0 36.0 36.0 36.0 33 34.999103046466956 36.0 36.0 36.0 36.0 36.0 34 34.968095971382155 36.0 36.0 36.0 36.0 36.0 35 34.948749292226935 36.0 36.0 36.0 36.0 36.0 36 34.909561154238965 36.0 36.0 36.0 36.0 36.0 37 34.86559455583602 36.0 36.0 36.0 36.0 36.0 38 34.844906415202175 36.0 36.0 36.0 36.0 36.0 39 34.740108375270545 36.0 36.0 36.0 32.0 36.0 40 34.787017192932325 36.0 36.0 36.0 32.0 36.0 41 34.76832404629233 36.0 36.0 36.0 32.0 36.0 42 34.7290935742143 36.0 36.0 36.0 32.0 36.0 43 34.74894826245019 36.0 36.0 36.0 32.0 36.0 44 34.65383414561869 36.0 36.0 36.0 32.0 36.0 45 34.61986103834939 36.0 36.0 36.0 32.0 36.0 46 34.531303413715186 36.0 36.0 36.0 32.0 36.0 47 34.51827245162007 36.0 36.0 36.0 32.0 36.0 48 34.46595545395374 36.0 36.0 36.0 32.0 36.0 49 34.42991062215237 36.0 36.0 36.0 32.0 36.0 50 34.041868415064584 36.0 36.0 36.0 32.0 36.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 21 9.0 22 16.0 23 44.0 24 151.0 25 301.0 26 724.0 27 1532.0 28 2808.0 29 4718.0 30 7233.0 31 10635.0 32 16215.0 33 27127.0 34 61756.0 35 244677.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 37.958404793664904 23.868809417942366 13.73599906836901 24.436786720023715 2 12.874844875808034 23.323004950743933 42.803957419897706 20.998192753550327 3 16.05656907751128 27.740279670322405 32.905846088716615 23.297305163449707 4 9.299501250181905 20.09816243104155 43.90718226196933 26.69515405680721 5 9.600048684203562 41.88614246479656 38.10756033930773 10.406248511692146 6 26.01701305745545 44.244003757160435 19.366574501580917 10.372408683803199 7 24.560386933583104 35.29816428801998 23.126319632963437 17.015129145433473 8 22.331497092177187 39.811507464029255 21.090049901308653 16.766945542484905 9 24.37967328666 16.512411826028057 23.31153127695491 35.79638361035703 10 13.540029528027814 29.044890010742275 36.42557402380234 20.98950643742757 11 30.496949299635396 25.251226365671286 27.91853862721129 16.33328570748202 12 20.524360622946137 29.375625089298474 32.841199536441714 17.258814751313682 13 24.883184370253954 23.19828758605727 30.008784323686456 21.90974372000233 14 18.381726490027674 26.460658400935582 29.353399692019494 25.804215417017247 15 21.67161446344187 33.46218771993883 24.661459573589877 20.20473824302943 16 20.49948934503871 30.204314902128875 29.42589682123901 19.870298931593403 17 17.483185428606202 31.403163414879376 29.88971969540622 21.2239314611082 18 17.356758258476763 30.956885261082963 32.76534945560862 18.921007024831653 19 21.423430860493298 29.647357029840244 29.20575955295201 19.723452556714452 20 19.739857016610838 31.986050917326814 29.880723701269496 18.393368364792853 21 21.403586755779926 28.363311160853666 28.621549110190347 21.611552973176064 22 19.908399612643077 30.9454260661576 29.358162277150708 19.78801204404862 23 20.779478495548297 30.845493391895644 29.39581155988305 18.97921655267301 24 21.0457243100077 30.890699292743008 29.352884989376594 18.710691407872698 25 20.35613647488391 30.55206445382265 30.758708277659448 18.333090793633993 26 17.60243635266787 30.755512750633695 33.014589540194 18.627461356504437 27 18.32431088039032 32.10802601429834 30.081281452905973 19.48638165240537 28 18.291960142032377 30.63591768049066 30.839652645114857 20.232469532362106 29 19.261532762703567 29.692944740636868 29.462487928137694 21.583034568521875 30 19.747157640173256 29.72088383698071 30.610435965211686 19.921522557634354 31 20.802023580212943 28.436487945304066 30.600829752555935 20.160658721927057 32 17.923120478803625 31.415137772462444 30.621365183017502 20.040376565716432 33 19.45918056860125 31.405892391750122 30.32637023905595 18.80855680059268 34 19.128382472396485 30.06024749894825 32.55984399681432 18.251526031840946 35 19.872151048033317 31.589697999185066 29.71985415905976 18.818296793721853 36 18.62498147873759 30.037254196388886 30.569608195923202 20.76815612895032 37 20.6901113671783 29.080089644096002 28.42813258224211 21.801666406483587 38 19.686674815979003 31.17455932858133 29.907182507553987 19.231583347885678 39 19.412877535091084 30.907671751180725 28.191932688618714 21.487518025109473 40 20.71856826107433 30.646706143205645 30.65728966571944 17.97743593000058 41 20.587130232312006 29.06254961104937 31.680425464359423 18.6698946922792 42 19.817266780974155 31.996306174721113 29.002349653902332 19.184077390402404 43 17.70108433926218 31.961283425855857 30.471044596032026 19.866587638849932 44 18.33740378432872 31.045942734972627 30.292093446617997 20.324560034080655 45 18.70495221362573 31.38845083242488 30.776700570472997 19.129896383476392 46 20.651489413495476 29.415453491879536 28.832812078299387 21.1002450163256 47 21.037884119785573 31.161395134703206 28.255922866471572 19.544797879039642 48 19.55858499527679 31.3282405582119 28.157207458701688 20.95596698780962 49 19.58447551411365 30.137696728512008 30.194320671443542 20.0835070859308 50 17.987945240274964 31.27225870636235 29.28147705203444 21.458319001328245 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 259.0 1 137.0 2 15.0 3 65.0 4 115.0 5 103.5 6 92.0 7 68.0 8 44.0 9 66.5 10 89.0 11 156.0 12 223.0 13 447.5 14 672.0 15 906.0 16 1140.0 17 1696.5 18 2253.0 19 2300.0 20 2347.0 21 3109.5 22 3872.0 23 4147.0 24 4422.0 25 6805.0 26 9188.0 27 13439.0 28 17690.0 29 19110.5 30 20531.0 31 20802.5 32 21074.0 33 23830.5 34 26587.0 35 30110.5 36 33634.0 37 37074.0 38 40514.0 39 39185.0 40 37856.0 41 36881.0 42 35906.0 43 29525.0 44 23144.0 45 21430.0 46 19716.0 47 18079.5 48 16443.0 49 13852.0 50 11261.0 51 11128.0 52 10995.0 53 9981.0 54 8967.0 55 8026.0 56 7085.0 57 7064.0 58 7043.0 59 5723.5 60 4404.0 61 3877.0 62 3350.0 63 2785.0 64 2220.0 65 1922.5 66 1625.0 67 1365.0 68 1105.0 69 974.5 70 844.0 71 659.5 72 475.0 73 403.0 74 331.0 75 247.0 76 163.0 77 138.5 78 114.0 79 86.5 80 59.0 81 47.0 82 35.0 83 27.5 84 20.0 85 16.5 86 13.0 87 8.0 88 3.0 89 3.5 90 4.0 91 3.0 92 2.0 93 1.0 94 0.0 95 0.0 96 0.0 97 0.0 98 0.0 99 1.0 100 2.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.03016303916432506 2 0.0060855254454340035 3 2.645880628449567E-4 4 2.645880628449567E-4 5 0.0 6 2.645880628449567E-4 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 2.645880628449567E-4 19 0.0 20 0.0 21 0.0 22 0.0 23 2.645880628449567E-4 24 0.001852116439914697 25 2.645880628449567E-4 26 0.0010583522513798267 27 0.0 28 0.0010583522513798267 29 2.645880628449567E-4 30 7.9376418853487E-4 31 5.291761256899134E-4 32 0.0010583522513798267 33 2.645880628449567E-4 34 0.0013229403142247834 35 0.0 36 5.291761256899134E-4 37 0.001852116439914697 38 0.0 39 2.645880628449567E-4 40 0.0 41 0.00158752837706974 42 0.00476258513120922 43 0.005291761256899134 44 0.0050271731940541775 45 0.003704232879829394 46 0.00317505675413948 47 0.0010583522513798267 48 0.006614701571123917 49 0.003704232879829394 50 0.0010583522513798267 >>END_MODULE >>Sequence Length Distribution pass #Length Count 50 377946.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 33.20928798828508 #Duplication Level Percentage of deduplicated Percentage of total 1 72.05385248636848 23.928571378852215 2 13.899117187344014 9.231595709148605 3 4.979905246885432 4.961373224945705 4 2.2225721330434776 2.952401521639115 5 1.256132677674602 2.0857635922195765 6 0.8161277828783289 1.6261813546108208 7 0.6133327025308937 1.4257839645687138 8 0.43943172212151393 1.1674571688897133 9 0.352495928492948 1.0535524923618227 >10 2.6460950262305225 17.95582851228645 >50 0.41150834836035916 9.511306972391525 >100 0.28390886049668185 18.204778733835898 >500 0.023924903974439485 5.211051039700823 >1k 0.0015949935982959656 0.6843543345490408 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source CCATAGGGTCTTCTCGTCTTATTATTATATTTCAGCCTCTTCACTGAAAG 1529 0.40455514808993875 No Hit GTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTTTAACGGCCGCGGTAT 1055 0.2791404063014293 No Hit ATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTA 831 0.219872680224159 No Hit CTCTAATACTTGTAATGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGT 784 0.20743704127044604 No Hit GTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTC 766 0.20267445613923685 No Hit ACCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATC 763 0.20188069195070196 No Hit ATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGACT 753 0.1992348113222524 No Hit CCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGA 736 0.1947368142538881 No Hit CTTCTACACCATTGGGATGTCCTGATCCAACATCGAGGTCGTAAACCCTA 727 0.19235552168828352 No Hit GACTATAGGCAATAATCACACTATAAATAATCCACCTATAACTTCTCTGT 727 0.19235552168828352 No Hit GTTATATAATTTAAGCTCCATAGGGTCTTCTCGTCTTATTATTATATTTC 726 0.19209093362543855 No Hit CCTATAACTTCTCTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCC 720 0.1905034052483688 No Hit GCCTAAAGGAAAGATCCAAAAAGATAAAAGGAACTCGGCAAACAAGAACC 715 0.18918046493414403 No Hit GATTAAACCTTGTACCTTTTGCATAATGAACTAACTAGAAAACTTCTAAC 702 0.1857408201171596 No Hit GTTCATGCTAGTCCCTAATTAAGGAACAAGTGATTATGCTACCTTTGCAC 696 0.18415329174008988 No Hit CACTATAAATAATCCACCTATAACTTCTCTGTTAACCCAACACCGGAATG 690 0.1825657633630201 No Hit CTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTAAAAGTAAG 685 0.18124282304879533 No Hit ATCTATAACTTTATAGATGCAACACTGTTAGTATGAGTAACAAGAATTCC 682 0.18044905886026044 No Hit CTGTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAA 673 0.17806776629465587 No Hit GTTATAGATTAACCCAATTTTAAGTTTAGGAAGTTGGTGTAAATTATGGA 621 0.1643091870267181 No Hit GATTAAAGATAAGAGACAGTTGGACCCTCGTTTAGCCGTTCATGCTAGTC 614 0.1624570705868034 No Hit GGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCT 598 0.1582236615812841 No Hit ATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGAT 585 0.15478401676429968 No Hit CCTCTAGCATTACAAGTATTAGAGGCACTGCCTGCCCAGTGACTAAAGTT 581 0.15372566451291983 No Hit CTGTTAACCCAACACCGGAATGCCTAAAGGAAAGATCCAAAAAGATAAAA 574 0.15187354807300515 No Hit TACTAACAGTGTTGCATCTATAAAGTTATAGATTAACCCAATTTTAAGTT 566 0.1497568435702455 No Hit CATTTACACCTACTACCCAACTATCCATAAATCTAAGTATAGCCATTCCA 552 0.14605261069041608 No Hit CTATTAAAGGTTTTTTCCGTTCCAGAAGAGCTGTCCCTCTTTTGGCTATA 530 0.14023167330782704 No Hit GTATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACC 527 0.13943790911929219 No Hit GTTAGTATGAGTAACAAGAATTCCAATTCTCCAGGCATACGCGTATAACA 520 0.13758579267937748 No Hit GTATCCTGACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAATTAGGGA 517 0.13679202849084263 No Hit GTACATGGGAGAAATCGTAAATAGATAGAAACCGACCTGGATTGCTCCGG 515 0.1362628523651527 No Hit CTTTATTGGTGGCTGCTTTTAGGCCTACAATGGTTAAAAGCTGTTTTGTT 489 0.12938356273118382 No Hit GATATATTTTGATCAACGGACCAAGTTACCCTAGGGATAACAGCGCAATC 486 0.12858979854264896 No Hit ACCTTTGCACGGTCAGGATACCGCGGCCGTTAAACTTTAGTCACTGGGCA 481 0.12726685822842418 No Hit GTATAGGGGTCCTAGGAAGATAATAGTTGTTAGGGCGTTTATTAGAATAA 480 0.12700227016557922 No Hit GACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTT 478 0.1264730940398893 No Hit GGATACCGCGGCCGTTAAACTTTAGTCACTGGGCAGGCAGTGCCTCTAAT 469 0.12409180147428468 No Hit GATATACACTGTTCTACAAATCCCGTTTCCAACGAATGTGTTTTTCAGTG 465 0.12303344922290485 No Hit GATAGAAACCGACCTGGATTGCTCCGGTCTGAACTCAGATCACGTAGGAC 458 0.12118133278299016 No Hit AAATATATCTGGGTCAATAAGATATGTTGATTTTACTTTGACTTGTAAGT 452 0.11959380440592043 No Hit TGCTAGAGGTGATGTTTTTGGTAAACAGGCGGGGTTCTTGTTTGCCGAGT 444 0.11747709990316077 No Hit GCTATCACCAAGCTCGTTAGGCTTTTCACCTCTACCTAAAAATCTTCTCA 439 0.11615415958893599 No Hit ATTTCAGCCTCTTCACTGAAAGGTCAATTTCACTGATTAAAGATAAGAGA 424 0.11218533864626164 No Hit AGCATGAACGGCTAAACGAGGGTCCAACTGTCTCTTATCTTTAATCAGTG 411 0.10874569382927719 No Hit GTATTGGAATTAGTGAAATTGGAGTTCCTTGTGGAAGGAAGTGGGCAAGT 411 0.10874569382927719 No Hit CTGTAGGACGTGGAATATGGCAAGAAAACTGAAAATCATGGAAAATGAGA 403 0.10662898932651754 No Hit GTTTAAAATTGAACTTAAATTCATTTTTTGGGTAACCAGCTATCACCAAG 398 0.10530604901229276 No Hit GTTATCCCTAGGGTAACTTGGTCCGTTGATCAAAATATATCTGGGTCAAT 395 0.10451228482375788 No Hit TATCAACGCAGAGTACATGGGAGAAATCGTAAATAGATAGAAACCGACCT 395 0.10451228482375788 No Hit CTTAAATAGGATTGCGCTGTTATCCCTAGGGTAACTTGGTCCGTTGATCA 393 0.10398310869806798 No Hit TCTAGGAGCTATAGAACTAGTACCGCAAGGGAAAGATGAAAGACTAATTA 392 0.10371852063522302 No Hit GGATTGCTCCGGTCTGAACTCAGATCACGTAGGACTTTAATCGTTGAACA 391 0.10345393257237807 No Hit GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 384 0.10160181613246336 No Hit GTCCTGATCCAACATCGAGGTCGTAAACCCTAATTGTCGATATGAACTCT 383 0.10133722806961842 No Hit ATTATAACCTAGACTTACAAGTCAAAGTAAAATCAACATATCTTATTGAC 383 0.10133722806961842 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 2.645880628449567E-4 0.0 0.0 12 0.0 0.0 2.645880628449567E-4 0.0 0.0 13 0.0 0.0 2.645880628449567E-4 0.0 0.0 14 0.0 0.0 2.645880628449567E-4 0.0 0.0 15 0.0 0.0 2.645880628449567E-4 0.0 0.0 16 0.0 0.0 2.645880628449567E-4 0.0 0.0 17 0.0 0.0 2.645880628449567E-4 0.0 0.0 18 0.0 0.0 2.645880628449567E-4 2.645880628449567E-4 0.0 19 0.0 0.0 2.645880628449567E-4 2.645880628449567E-4 0.0 20 0.0 0.0 2.645880628449567E-4 2.645880628449567E-4 0.0 21 0.0 0.0 2.645880628449567E-4 2.645880628449567E-4 0.0 22 0.0 0.0 2.645880628449567E-4 2.645880628449567E-4 0.0 23 0.0 0.0 2.645880628449567E-4 2.645880628449567E-4 0.0 24 0.0 0.0 2.645880628449567E-4 2.645880628449567E-4 0.0 25 0.0 0.0 2.645880628449567E-4 5.291761256899134E-4 0.0 26 0.0 0.0 2.645880628449567E-4 7.937641885348701E-4 0.0 27 0.0 0.0 2.645880628449567E-4 0.0015875283770697402 0.0 28 0.0 0.0 5.291761256899134E-4 0.001852116439914697 0.0 29 0.0 0.0 5.291761256899134E-4 0.002645880628449567 0.0 30 0.0 0.0 5.291761256899134E-4 0.004497997068364264 0.0 31 0.0 0.0 5.291761256899134E-4 0.008995994136728528 0.0 32 0.0 0.0 5.291761256899134E-4 0.012700227016557921 0.0 33 0.0 0.0 5.291761256899134E-4 0.016933636022077227 0.0 34 0.0 0.0 5.291761256899134E-4 0.02222539727897636 0.0 35 0.0 0.0 5.291761256899134E-4 0.02804633466156541 0.0 36 0.0 0.0 5.291761256899134E-4 0.034661036232689325 0.0 37 0.0 0.0 5.291761256899134E-4 0.050536320003386724 0.0 38 0.0 0.0 5.291761256899134E-4 0.06297195895709969 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCTAGAG 45 4.062648E-5 29.332893 3 GATAACT 30 0.0057405047 29.33289 24 AGATAAC 30 0.0057405047 29.33289 23 AGACATC 30 0.0057405047 29.33289 38 TAGTCGG 55 4.949792E-6 27.99958 40 ATAGGAC 75 6.986738E-8 26.399605 3 ACCTACT 135 0.0 26.07368 8 GACGTGA 190 0.0 25.4733 7 TACATTG 55 1.5909558E-4 23.99964 5 AGGACCT 340 0.0 23.940815 5 CCTACAG 570 0.0 23.929464 3 TAGGACC 250 0.0 23.759644 4 CAGTGAT 280 0.0 23.571074 37 ACTGTTG 75 2.058292E-6 23.466316 26 GTTATAT 170 0.0 23.309187 1 AGGGGTC 85 2.3441135E-7 23.293766 5 GTATAGG 95 2.6546331E-8 23.172876 1 TCCTACA 630 0.0 23.047272 2 GGACCTG 355 0.0 22.929234 6 GTATCAA 675 0.0 22.503439 1 >>END_MODULE