FastQCFastQC Report
Thu 2 Feb 2017
SRR4062094_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062094_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720078
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT12780.1774807729162674No Hit
GTCCTAAAGTGTGTATTTCTCATTT11200.15553870552912324No Hit
CTGTAGGACGTGGAATATGGCAAGA11070.15373334555423163No Hit
GTCCTACAGTGGACATTTCTAAATT10650.1479006440968895No Hit
CTTTAGGACGTGAAATATGGCGAGG8760.12165348753884996No Hit
TATCAACGCAGAGTACTTTTTTTTT8580.1191537583428462No Hit
GCGCAAGACGGACCAGAGCGAAAGC7240.10054466321704038No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGCG508.689713E-515.2028397
CCAACGT456.778613E-414.77129119
CGCGTAA400.005266491314.25365210
GGTGTGC400.005268922514.252668
TCGCGTA400.005268922514.252669
ATCGCCA1203.6379788E-1214.24869616
GATTTAT400.00529572914.2417641
GTCTTAG954.9240043E-913.9919091
GGTATCA3200.013.9450611
AGAACCG551.9547483E-413.8207625
GATATAC3450.013.2097531
TAGGACA1950.013.15630154
CGCATCG1251.0186341E-1012.92241213
CATCGCC1403.6379788E-1212.89347115
TAATATA1051.9814252E-812.6690324
GGGTCGC604.080841E-412.6690326
TAGAACC1051.9814252E-812.6690324
GTCTTCG1352.7284841E-1112.66903111
TCCAACG1207.421477E-1012.66198618
GCATCGC1457.2759576E-1212.44886814
Produced by FastQC (version 0.11.5)