##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062094_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720078
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.205006957579595	32.0	32.0	32.0	32.0	32.0
2	30.729601515391387	32.0	32.0	32.0	32.0	32.0
3	30.75858587541905	32.0	32.0	32.0	32.0	32.0
4	30.771381711425708	32.0	32.0	32.0	32.0	32.0
5	30.692887437194305	32.0	32.0	32.0	32.0	32.0
6	34.34039784578893	36.0	36.0	36.0	32.0	36.0
7	34.264596335397	36.0	36.0	36.0	32.0	36.0
8	34.21504753651688	36.0	36.0	36.0	32.0	36.0
9	34.33640522276753	36.0	36.0	36.0	32.0	36.0
10	34.02502506672888	36.0	36.0	36.0	32.0	36.0
11	34.3335888612067	36.0	36.0	36.0	32.0	36.0
12	34.147306264043614	36.0	36.0	36.0	32.0	36.0
13	34.22780587658559	36.0	36.0	36.0	32.0	36.0
14	34.141337466218936	36.0	36.0	36.0	32.0	36.0
15	34.08720027552571	36.0	36.0	36.0	32.0	36.0
16	34.10083213207458	36.0	36.0	36.0	32.0	36.0
17	34.03399631706565	36.0	36.0	36.0	32.0	36.0
18	34.045456186690885	36.0	36.0	36.0	32.0	36.0
19	34.00993364607723	36.0	36.0	36.0	32.0	36.0
20	33.99765858698641	36.0	36.0	36.0	32.0	36.0
21	33.963784478903676	36.0	36.0	36.0	32.0	36.0
22	33.934808451306665	36.0	36.0	36.0	32.0	36.0
23	33.89219640094545	36.0	36.0	36.0	32.0	36.0
24	33.85306452912046	36.0	36.0	36.0	32.0	36.0
25	33.50379264468572	36.0	36.0	36.0	27.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
4	9.0
5	53.0
6	166.0
7	33.0
8	118.0
9	92.0
10	48.0
11	16.0
12	16.0
13	28.0
14	112.0
15	146.0
16	260.0
17	334.0
18	445.0
19	622.0
20	997.0
21	1464.0
22	2215.0
23	3271.0
24	4556.0
25	6393.0
26	9049.0
27	11461.0
28	15246.0
29	20270.0
30	26239.0
31	36008.0
32	50017.0
33	69867.0
34	149826.0
35	310701.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.761912697619124	17.66976080169761	11.766184492661845	25.802142008021423
2	15.883579117233296	19.524717495490066	39.400369682686716	25.191333704589923
3	19.468605004812055	23.099794880198647	28.42471603336912	29.006884081620182
4	12.585363279172125	15.634761135593761	35.77695340765627	36.002922177577844
5	14.414436942568976	36.89769664638382	33.88374871495652	14.80411769609069
6	34.44002084418968	35.50265763418447	16.64825429911412	13.409067222511725
7	30.10443109243931	30.338678151491816	20.267583821521814	19.289306934547064
8	27.096688089885767	35.28130313229428	19.05623838301268	18.565770394807267
9	26.69568699201686	14.518967947880201	18.84629608517572	39.93904897492722
10	16.434523735033075	27.34350340462395	31.352714035226768	24.869258825116212
11	36.98008146531883	21.08502681902823	22.65300474634915	19.28188696930379
12	24.54473652008499	23.493903863482142	29.717274982479168	22.2440846339537
13	29.942670102103676	20.075336741887533	24.98612986341218	24.995863292596614
14	22.64240132356417	20.681663353121916	25.219041528216106	31.456893795097805
15	24.52419059908808	29.160264370229566	21.86491421038653	24.450630820295824
16	24.640141482479446	26.034389029546425	25.445712911249498	23.87975657672463
17	23.04721226960087	26.924215944307957	26.01700959761749	24.011562188473683
18	23.778918700238147	25.736652666964176	27.718159713388413	22.76626891940926
19	25.452184888434186	25.054490155630553	25.242425590356355	24.250899365578903
20	25.468248088435818	25.39416237974972	25.387212500886108	23.75037703092835
21	25.84095840277324	24.935401773862427	24.44724906768671	24.776390755677625
22	25.295444845893243	25.861103040430173	25.24178447157738	23.601667642099205
23	23.84763884970278	25.725607052665666	25.875155142244417	24.551598955387135
24	24.637010349687674	25.335329324671065	26.10717750701238	23.920482818628884
25	24.414211809445444	25.444987754670876	26.0415479430349	24.099252492848784
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	408.0
1	408.0
2	322.5
3	237.0
4	237.0
5	237.0
6	537.0
7	837.0
8	837.0
9	837.0
10	1014.0
11	1191.0
12	1191.0
13	1191.0
14	1569.5
15	1948.0
16	1948.0
17	1948.0
18	3227.0
19	4506.0
20	4506.0
21	4506.0
22	7800.5
23	11095.0
24	11095.0
25	11095.0
26	17412.5
27	23730.0
28	23730.0
29	23730.0
30	31754.5
31	39779.0
32	39779.0
33	39779.0
34	45689.0
35	51599.0
36	51599.0
37	51599.0
38	57786.5
39	63974.0
40	63974.0
41	63974.0
42	71327.5
43	78681.0
44	78681.0
45	78681.0
46	85128.0
47	91575.0
48	91575.0
49	91575.0
50	92127.0
51	92679.0
52	92679.0
53	92679.0
54	85610.5
55	78542.0
56	78542.0
57	78542.0
58	72211.5
59	65881.0
60	65881.0
61	65881.0
62	57391.5
63	48902.0
64	48902.0
65	48902.0
66	40261.0
67	31620.0
68	31620.0
69	31620.0
70	23934.5
71	16249.0
72	16249.0
73	16249.0
74	12247.5
75	8246.0
76	8246.0
77	8246.0
78	6574.5
79	4903.0
80	4903.0
81	4903.0
82	3401.5
83	1900.0
84	1900.0
85	1900.0
86	1395.5
87	891.0
88	891.0
89	891.0
90	622.0
91	353.0
92	353.0
93	353.0
94	217.5
95	82.0
96	82.0
97	82.0
98	176.0
99	270.0
100	270.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	8.332430653345888E-4
2	1.3887384422243146E-4
3	0.0015276122864467461
4	0.009304547562902908
5	0.03582945180938731
6	0.06290985143276145
7	0.10443313085526847
8	0.10623849083016006
9	0.11276556150861435
10	0.12623632439819021
11	0.13873497037820903
12	0.12970817050375097
13	0.12595857670974533
14	0.11221006613172461
15	0.12984704434797342
16	0.11623740761417514
17	0.11623740761417514
18	0.10790497696082924
19	0.09471196175969825
20	0.08901813414657857
21	0.08762939570435425
22	0.10262777088037685
23	0.08013020811634296
24	0.08832376492546641
25	0.0861017834179075
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	720078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	59.47181570354356
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.26102892787709	47.13797304871941
2	13.201930949087334	15.702856086700596
3	3.6339432916461614	6.483516171537268
4	1.413113474269123	3.361616964397097
5	0.704839063687875	2.0959029448151747
6	0.3900124941406923	1.3916850704168768
7	0.24206083923486074	1.0077058334014506
8	0.16805479587781688	0.7995619078833724
9	0.1186732937491036	0.6351944629301259
>10	0.709344905223273	8.094834543152333
>50	0.08659551591956723	3.5541883734515305
>100	0.06477025334444679	7.335835659479885
>500	0.0046934966188893505	1.7613151329791292
>1k	9.386993237778702E-4	0.6378138001358654
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1278	0.1774807729162674	No Hit
GTCCTAAAGTGTGTATTTCTCATTT	1120	0.15553870552912324	No Hit
CTGTAGGACGTGGAATATGGCAAGA	1107	0.15373334555423163	No Hit
GTCCTACAGTGGACATTTCTAAATT	1065	0.1479006440968895	No Hit
CTTTAGGACGTGAAATATGGCGAGG	876	0.12165348753884996	No Hit
TATCAACGCAGAGTACTTTTTTTTT	858	0.1191537583428462	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	724	0.10054466321704038	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.3887384422243146E-4
2	0.0	0.0	0.0	0.0	1.3887384422243146E-4
3	0.0	0.0	0.0	0.0	1.3887384422243146E-4
4	0.0	0.0	0.0	0.0	1.3887384422243146E-4
5	0.0	0.0	0.0	0.0	1.3887384422243146E-4
6	0.0	0.0	0.0	0.0	1.3887384422243146E-4
7	1.3887384422243146E-4	0.0	0.0	0.0	1.3887384422243146E-4
8	1.3887384422243146E-4	0.0	0.0	0.0	1.3887384422243146E-4
9	1.3887384422243146E-4	0.0	0.0	0.0	1.3887384422243146E-4
10	1.3887384422243146E-4	0.0	0.0	0.0	1.3887384422243146E-4
11	1.3887384422243146E-4	0.0	0.0	0.0	1.3887384422243146E-4
12	1.3887384422243146E-4	0.0	0.0	0.0	1.3887384422243146E-4
13	1.3887384422243146E-4	0.0	0.0	0.0	1.3887384422243146E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACCGCG	50	8.689713E-5	15.202839	7
CCAACGT	45	6.778613E-4	14.771291	19
CGCGTAA	40	0.0052664913	14.253652	10
GGTGTGC	40	0.0052689225	14.25266	8
TCGCGTA	40	0.0052689225	14.25266	9
ATCGCCA	120	3.6379788E-12	14.248696	16
GATTTAT	40	0.005295729	14.241764	1
GTCTTAG	95	4.9240043E-9	13.991909	1
GGTATCA	320	0.0	13.945061	1
AGAACCG	55	1.9547483E-4	13.820762	5
GATATAC	345	0.0	13.209753	1
TAGGACA	195	0.0	13.1563015	4
CGCATCG	125	1.0186341E-10	12.922412	13
CATCGCC	140	3.6379788E-12	12.893471	15
TAATATA	105	1.9814252E-8	12.669032	4
GGGTCGC	60	4.080841E-4	12.669032	6
TAGAACC	105	1.9814252E-8	12.669032	4
GTCTTCG	135	2.7284841E-11	12.669031	11
TCCAACG	120	7.421477E-10	12.661986	18
GCATCGC	145	7.2759576E-12	12.448868	14
>>END_MODULE