FastQCFastQC Report
Thu 2 Feb 2017
SRR4062094_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062094_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences720078
Sequences flagged as poor quality0
Sequence length50
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATACCG401.8192912E-532.999385
CACGCGA300.00574402229.33277710
GTTATAT701.1279735E-625.1511121
CCGTGCG450.001397819524.4439819
GTATAGA651.8074708E-523.7000891
GTATCAA9400.023.4119381
GTCTAGT602.8648466E-422.0072231
ATTGTCG500.00257867722.00111238
TCGATCG500.00257867722.00111241
TGTAGGA8650.020.6007652
GTCCTAC6350.020.4476591
GTAGTAC654.9185957E-420.3143621
ATAGTAG654.9301283E-420.3073083
CTGTAGG8350.020.0305271
CGATCGT550.004480572420.00100942
AACCGTC550.004482373619.999627
CCACGCG550.004482373619.999629
GTCCTAA4300.019.9600411
GGTATCA4550.019.8306851
GTAGGAC8400.019.6424853