##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR4062094_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	720078
Sequences flagged as poor quality	0
Sequence length	50
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.248823044170216	32.0	32.0	32.0	32.0	32.0
2	31.433882162765702	32.0	32.0	32.0	32.0	32.0
3	31.56709273162074	32.0	32.0	32.0	32.0	32.0
4	31.64658273131522	32.0	32.0	32.0	32.0	32.0
5	31.62336302456123	32.0	32.0	32.0	32.0	32.0
6	35.269531911820664	36.0	36.0	36.0	36.0	36.0
7	35.26924860917845	36.0	36.0	36.0	36.0	36.0
8	35.22113715458603	36.0	36.0	36.0	36.0	36.0
9	35.30526137446221	36.0	36.0	36.0	36.0	36.0
10	35.177614091806724	36.0	36.0	36.0	36.0	36.0
11	35.30086601729257	36.0	36.0	36.0	36.0	36.0
12	35.215964103888744	36.0	36.0	36.0	36.0	36.0
13	35.25023539116596	36.0	36.0	36.0	36.0	36.0
14	35.22590052744286	36.0	36.0	36.0	36.0	36.0
15	35.205656887170555	36.0	36.0	36.0	36.0	36.0
16	35.21199092320554	36.0	36.0	36.0	36.0	36.0
17	35.19757998439058	36.0	36.0	36.0	36.0	36.0
18	35.18404256205578	36.0	36.0	36.0	36.0	36.0
19	35.16642780365461	36.0	36.0	36.0	36.0	36.0
20	35.16437941445232	36.0	36.0	36.0	36.0	36.0
21	35.15147525684718	36.0	36.0	36.0	36.0	36.0
22	35.11907737772852	36.0	36.0	36.0	36.0	36.0
23	35.091430372820724	36.0	36.0	36.0	36.0	36.0
24	35.07580012165349	36.0	36.0	36.0	36.0	36.0
25	35.04363416185469	36.0	36.0	36.0	36.0	36.0
26	35.01309858098706	36.0	36.0	36.0	36.0	36.0
27	34.98946780765417	36.0	36.0	36.0	36.0	36.0
28	34.955690911262394	36.0	36.0	36.0	36.0	36.0
29	34.926514905329704	36.0	36.0	36.0	36.0	36.0
30	34.88939114929216	36.0	36.0	36.0	32.0	36.0
31	34.899981668652565	36.0	36.0	36.0	32.0	36.0
32	34.859576323676045	36.0	36.0	36.0	32.0	36.0
33	34.85499487555515	36.0	36.0	36.0	32.0	36.0
34	34.838017548098954	36.0	36.0	36.0	32.0	36.0
35	34.82542585664331	36.0	36.0	36.0	32.0	36.0
36	34.778622593663464	36.0	36.0	36.0	32.0	36.0
37	34.7427806432081	36.0	36.0	36.0	32.0	36.0
38	34.72052333219457	36.0	36.0	36.0	32.0	36.0
39	34.657587094731404	36.0	36.0	36.0	32.0	36.0
40	34.67333955488156	36.0	36.0	36.0	32.0	36.0
41	34.660404845030676	36.0	36.0	36.0	32.0	36.0
42	34.62014392885215	36.0	36.0	36.0	32.0	36.0
43	34.62985815425551	36.0	36.0	36.0	32.0	36.0
44	34.55424967850705	36.0	36.0	36.0	32.0	36.0
45	34.54013315224184	36.0	36.0	36.0	32.0	36.0
46	34.474384719433175	36.0	36.0	36.0	32.0	36.0
47	34.46535097586651	36.0	36.0	36.0	32.0	36.0
48	34.42754951546916	36.0	36.0	36.0	32.0	36.0
49	34.401617602537506	36.0	36.0	36.0	32.0	36.0
50	34.015774679965226	36.0	36.0	36.0	32.0	36.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	3.0
20	10.0
21	18.0
22	52.0
23	126.0
24	360.0
25	869.0
26	1809.0
27	3588.0
28	6308.0
29	9700.0
30	14768.0
31	21838.0
32	32544.0
33	55187.0
34	121922.0
35	450976.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.51700071681401	18.17790323566511	12.203202880592121	26.101893166928757
2	15.182746538044361	19.89997791792412	39.242209818607556	25.675065725423966
3	18.823039266743045	23.631288407457557	28.799777800923515	28.74589452487588
4	12.21812081468952	16.026874866333927	36.104977516324624	35.65002680265194
5	14.10861045608948	37.175694855279566	33.69315546371365	15.0225392249173
6	34.483625385027736	35.54948213943489	16.58334236013321	13.383550115404164
7	30.132846719383178	30.298384341696316	20.358905563008452	19.209863375912054
8	27.032765894805838	35.36436330508639	19.058074264176934	18.54479653593083
9	26.85847922030669	14.34747346815206	18.791714230958313	40.002333080582936
10	16.333647188221274	27.410780498779296	31.356325286982802	24.899247026016628
11	37.09681451176123	20.98689308658229	22.658656423331916	19.25763597832457
12	24.51637183749538	23.384966628615235	29.784412244229095	22.31424928966029
13	29.957310180286022	19.954643802476955	25.231155513708238	24.856890503528785
14	22.825721657931503	20.382514116526266	25.324617610869932	31.4671466146723
15	24.43624162937904	29.09170950924761	21.999838906340702	24.47220995503265
16	24.64052305735244	25.97760785697026	25.398084085802292	23.983784999875013
17	23.074861334466544	26.831537694527537	26.131613519646486	23.961987451359438
18	23.735166475714337	25.675242162274763	27.783355900427036	22.806235461583864
19	25.526956801902017	24.788425698327128	25.445298981499224	24.23931851827163
20	25.5546991632816	25.153907579071628	25.56080963788494	23.73058361976183
21	25.817480884015342	24.742180708201055	24.740375348226166	24.699963059557437
22	25.42668988637342	25.58375620418899	25.437244298534324	23.55230961090326
23	23.956015876057872	25.459464113609915	25.990795441604934	24.593724568727275
24	24.798004627353603	25.22407668185972	26.110523202039815	23.867395488746865
25	24.574628233686443	25.101655936317833	26.100717146523422	24.222998683472298
26	23.318566250503423	26.560889913480633	26.6733789770439	23.447164858972044
27	24.196608116966082	25.619104756191046	26.18085413680854	24.00343299003433
28	23.699943755077665	26.03487185184671	25.83100136793206	24.434183025143565
29	23.788527290250723	25.24407213703036	25.755408471907053	25.21199210081186
30	23.609444611094446	25.76311813263118	26.157106511571065	24.470330744703308
31	24.98156442037288	25.06683331597403	25.413741624136378	24.53786063951672
32	24.209174108072826	25.889427416778926	25.365172830419265	24.536225644728983
33	23.628233170155887	25.627191611984863	26.474186716661457	24.270388501197793
34	24.323922713391557	25.80127292649495	26.45842852044168	23.41637583967181
35	24.53129947394442	25.782972908415225	26.28389225581744	23.40183536182292
36	23.449885845382987	26.052805537193297	26.1851526783284	24.312155939095316
37	24.312426048650963	25.906997672442046	25.413849803074157	24.366726475832838
38	24.009482306083505	26.093423212485312	25.627223717430613	24.26987076400057
39	25.108495642814983	24.852550081588724	25.595389369162934	24.44356490643336
40	25.70263221484339	25.15158080096878	25.73401770363766	23.411769280550164
41	24.390264225779305	25.24726900034164	26.734632658672957	23.627834115206106
42	24.999618080486414	26.309115932709947	25.88942110723328	22.801844879570364
43	23.933715438020016	25.637631987911902	26.42938585953417	23.999266714533913
44	23.5189140693789	26.10847084769584	25.7473828060985	24.625232276826758
45	23.846445489732062	26.337498281386058	25.669912243058533	24.146143985823347
46	23.920406964390615	25.38902846000975	25.663310015929163	25.027254559670475
47	24.79185849015989	25.489297464249656	25.86815320326138	23.850690842329076
48	24.57616879085716	25.878876005327488	25.052809639477864	24.492145564337488
49	24.465837931374494	25.902815487193305	25.280777942734773	24.35056863869743
50	24.1866717773093	26.716439881899717	24.74078765002097	24.356100690770013
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	232.0
1	127.0
2	22.0
3	69.0
4	116.0
5	117.5
6	119.0
7	97.0
8	75.0
9	101.0
10	127.0
11	232.0
12	337.0
13	636.5
14	936.0
15	1173.0
16	1410.0
17	1455.5
18	1501.0
19	1500.0
20	1499.0
21	1763.5
22	2028.0
23	2520.5
24	3013.0
25	3979.5
26	4946.0
27	6959.5
28	8973.0
29	10840.5
30	12708.0
31	14674.5
32	16641.0
33	19741.0
34	22841.0
35	26704.5
36	30568.0
37	33125.5
38	35683.0
39	35865.5
40	36048.0
41	36700.5
42	37353.0
43	37932.5
44	38512.0
45	41106.5
46	43701.0
47	46428.5
48	49156.0
49	50841.0
50	52526.0
51	51011.5
52	49497.0
53	48535.0
54	47573.0
55	48000.5
56	48428.0
57	46689.5
58	44951.0
59	40905.0
60	36859.0
61	32254.0
62	27649.0
63	23910.5
64	20172.0
65	17091.5
66	14011.0
67	11883.5
68	9756.0
69	8717.5
70	7679.0
71	6031.0
72	4383.0
73	3792.5
74	3202.0
75	2388.0
76	1574.0
77	1423.0
78	1272.0
79	1073.0
80	874.0
81	664.0
82	454.0
83	408.0
84	362.0
85	267.0
86	172.0
87	123.5
88	75.0
89	51.0
90	27.0
91	18.5
92	10.0
93	9.0
94	8.0
95	10.5
96	13.0
97	8.0
98	3.0
99	3.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03138548879426951
2	0.005138332236229964
3	4.166215326672944E-4
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	5.554953768897258E-4
17	0.0
18	4.166215326672944E-4
19	0.0
20	4.166215326672944E-4
21	0.0
22	0.0
23	0.0
24	0.0016664861306691775
25	2.777476884448629E-4
26	0.0011109907537794517
27	8.332430653345888E-4
28	0.001805359974891609
29	5.554953768897258E-4
30	8.332430653345888E-4
31	4.166215326672944E-4
32	0.0011109907537794517
33	4.166215326672944E-4
34	6.943692211121572E-4
35	2.777476884448629E-4
36	2.777476884448629E-4
37	0.0013887384422243145
38	0.0
39	4.166215326672944E-4
40	0.0
41	0.0016664861306691775
42	0.004305089170895375
43	0.004305089170895375
44	0.004443963015117807
45	0.0029163507286710604
46	0.002083107663336472
47	0.002083107663336472
48	0.005416079924674827
49	0.0034718461055607865
50	0.0016664861306691775
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
50	720078.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.88171799657849
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.45566567602273	49.963087622700826
2	13.303913069181366	16.73145819734515
3	3.680875927249444	6.943794061130816
4	1.3147258745018717	3.3068888673292696
5	0.6638650463195238	2.087248731522491
6	0.3619566452125449	1.3656273414745714
7	0.22181697986737614	0.9763762942411153
8	0.14845509535613277	0.7468089153071609
9	0.10956983506666186	0.6200945522634103
>10	0.6196364344277818	7.363077314188356
>50	0.06909048704561732	3.105702798659249
>100	0.047985237118646086	5.899005559443681
>500	0.00244369263027594	0.8908297443940142
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	2.777476884448629E-4	0.0
22	0.0	0.0	0.0	5.554953768897258E-4	0.0
23	0.0	0.0	0.0	9.721169095570202E-4	0.0
24	0.0	0.0	0.0	0.0013887384422243145	0.0
25	0.0	0.0	0.0	0.0019442338191140403	0.0
26	0.0	0.0	0.0	0.002777476884448629	0.0
27	0.0	0.0	0.0	0.0034718461055607865	0.0
28	0.0	0.0	0.0	0.004166215326672944	0.0
29	0.0	0.0	0.0	0.005832701457342122	0.0
30	0.0	0.0	0.0	0.010137790628237496	0.0
31	0.0	0.0	0.0	0.015414996708689892	0.0
32	0.0	0.0	0.0	0.023469679673590916	0.0
33	0.0	0.0	0.0	0.0295801288193779	0.0
34	0.0	0.0	0.0	0.03846805484961351	0.0
35	0.0	0.0	0.0	0.04568949474917995	0.0
36	0.0	0.0	0.0	0.05638278075430717	0.0
37	0.0	0.0	0.0	0.07596399278967	0.0
38	0.0	0.0	0.0	0.10026691552859551	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TATACCG	40	1.8192912E-5	32.99938	5
CACGCGA	30	0.005744022	29.332777	10
GTTATAT	70	1.1279735E-6	25.151112	1
CCGTGCG	45	0.0013978195	24.443981	9
GTATAGA	65	1.8074708E-5	23.700089	1
GTATCAA	940	0.0	23.411938	1
GTCTAGT	60	2.8648466E-4	22.007223	1
ATTGTCG	50	0.002578677	22.001112	38
TCGATCG	50	0.002578677	22.001112	41
TGTAGGA	865	0.0	20.600765	2
GTCCTAC	635	0.0	20.447659	1
GTAGTAC	65	4.9185957E-4	20.314362	1
ATAGTAG	65	4.9301283E-4	20.307308	3
CTGTAGG	835	0.0	20.030527	1
CGATCGT	55	0.0044805724	20.001009	42
AACCGTC	55	0.0044823736	19.99962	7
CCACGCG	55	0.0044823736	19.99962	9
GTCCTAA	430	0.0	19.960041	1
GGTATCA	455	0.0	19.830685	1
GTAGGAC	840	0.0	19.642485	3
>>END_MODULE