FastQCFastQC Report
Thu 2 Feb 2017
SRR4062093_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR4062093_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences145968
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGAAGCAGTGGTATCAACG41992.8766578976213966No Hit
GTACATGGGAAGCAGTGGTATCAAC40642.784171873287296No Hit
TCCATGTACTCTGCGTTGATACCAC38502.637564397676203No Hit
CCCATGTACTCTGCGTTGATACCAC37372.5601501699002522No Hit
CATGTACTCTGCGTTGATACCACTG19611.3434451386605282No Hit
GTATCAACGCAGAGTACATGGGAAG17041.1673791515948702No Hit
GAGTACATGGAAGCAGTGGTATCAA16291.1159980269648142No Hit
GAGTACATGGGAAGCAGTGGTATCA12890.8830702619752275No Hit
TATCAACGCAGAGTACATGGGAAGC12220.8371697906390441No Hit
GCTTCCATGTACTCTGCGTTGATAC12140.8316891373451716No Hit
ACTCTGCGTTGATACCACTGCTTCC10900.7467390112901459No Hit
GTACTTTTTTTTTTTTTTTTTTTTT9420.6453469253535021No Hit
GCGTTGATACCACTGCTTCCCATGT9400.643976762030034No Hit
GCTTCCCATGTACTCTGCGTTGATA9320.6384961087361614No Hit
GCGTTGATACCACTGCTTCCATGTA9300.6371259454126932No Hit
CAGTGGTATCAACGCAGAGTACATG8850.6062972706346597No Hit
ACGCAGAGTACATGGGAAGCAGTGG8790.6021867806642551No Hit
CATGGAAGCAGTGGTATCAACGCAG8730.5980762906938507No Hit
GGTATCAACGCAGAGTACATGGGAA7970.5460100844020608No Hit
GTATCAACGCAGAGTACATGGAAGC7530.5158664912857612No Hit
GTATCAACGCAGAGTACTTTTTTTT7390.5062753480214841No Hit
TATCAACGCAGAGTACATGGAAGCA6720.46037487668530086No Hit
TATCAACGCAGAGTACTTTTTTTTT5830.3994026087909679No Hit
ACGCAGAGTACATGGAAGCAGTGGT5790.39666228214403154No Hit
AAAAAGTACTCTGCGTTGATACCAC5740.3932368738353612No Hit
GTACTCTGCGTTGATACCACTGCTT5710.39118162885015895No Hit
GAGTACTTTTTTTTTTTTTTTTTTT4400.3014359311629946No Hit
GGTATCAACGCAGAGTACATGGAAG4260.29184478789871754No Hit
GTGGTATCAACGCAGAGTACATGGG4160.2849939712813767No Hit
AAGCAGTGGTATCAACGCAGAGTAC4060.278143154664036No Hit
ATACCACTGCTTCCATGTACTCTGC4040.2767729913405678No Hit
CTGCTTCCCATGTACTCTGCGTTGA3860.26444152142935434No Hit
ATACCACTGCTTCCCATGTACTCTG3650.2500548065329387No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3550.24320398991559794No Hit
GAAGCAGTGGTATCAACGCAGAGTA3510.24046366326866161No Hit
GCAGTGGTATCAACGCAGAGTACAT3360.23018743834265043No Hit
AAAGTACTCTGCGTTGATACCACTG2960.2027841718732873No Hit
GTGGTATCAACGCAGAGTACATGGA2940.2014140085498191No Hit
GGTATCAACGCAGAGTACTTTTTTT2850.19524827359421243No Hit
CCATGTACTCTGCGTTGATACCACT2830.19387811027074428No Hit
GGGAAGCAGTGGTATCAACGCAGAG2800.19182286528554204No Hit
CCACTGCTTCCATGTACTCTGCGTT2740.18771237531513754No Hit
CCACTGCTTCCCATGTACTCTGCGT2730.18702729365340348No Hit
TACCACTGCTTCCCATGTACTCTGC2590.17743615038912638No Hit
CATGGGAAGCAGTGGTATCAACGCA2580.1767510687273923No Hit
GTTGATACCACTGCTTCCATGTACT2490.17058533377178559No Hit
GATACCACTGCTTCCCATGTACTCT2440.1671599254631152No Hit
GATACCACTGCTTCCATGTACTCTG2420.16578976213964705No Hit
ATCAACGCAGAGTACATGGGAAGCA2410.16510468047791296No Hit
CTGCTTCCATGTACTCTGCGTTGAT2410.16510468047791296No Hit
ACATGGAAGCAGTGGTATCAACGCA2390.16373451715444481No Hit
GCAGAGTACATGGGAAGCAGTGGTA2360.16167927216924258No Hit
CCCCATGTACTCTGCGTTGATACCA2350.1609941905075085No Hit
GTCCTAAAGTGTGTATTTCTCATTT2310.15825386386057216No Hit
GTTGATACCACTGCTTCCCATGTAC2170.1486627205962951No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA2150.14729255727282692No Hit
TGGAAGCAGTGGTATCAACGCAGAG1980.13564616902334758No Hit
ACATGGGAAGCAGTGGTATCAACGC1920.1315356790529431No Hit
ATGGGAAGCAGTGGTATCAACGCAG1710.11714896415652747No Hit
CTTCCCATGTACTCTGCGTTGATAC1670.11440863750959114No Hit
CTGTAGGACGTGGAATATGGCAAGA1550.1061876575687822No Hit
CTTCCATGTACTCTGCGTTGATACC1490.10207716759837772No Hit
ATCAACGCAGAGTACATGGAAGCAG1470.10070700427490956No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1460.1000219226131755No Hit
GCAGAGTACATGGAAGCAGTGGTAT1460.1000219226131755No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGAGTT359.8503486E-519.00274511
ACTGTTC307.6872285E-418.996238
TGTATAT307.6872285E-418.996232
TGAGTTA402.7366335E-416.62740112
CAATGAG402.743809E-416.62179
ATACCAA350.002150523716.29365319
TTACAAT350.002155461316.28806716
GTTACAA350.002155461316.28806715
TATATCA350.002160408816.2824824
TCAATGA350.002160408816.2824828
GTATATC350.002160408816.2824823
CTGTTCT350.002160408816.2824829
CACTGTT456.720612E-414.7748457
AATGAGT456.720612E-414.77484510
ATATCAA400.005255732714.2471725
TGTTCTA551.9443309E-413.81543810
GAGTTAC500.00148659413.3019213
TATACAC500.001490444513.2973613
GATATAC500.001490444513.2973611
TCCTACA701.07989144E-412.2118622
Produced by FastQC (version 0.11.5)